acid-phosphatase and Lymphatic-Diseases

Topics: Acid Phosphatase; Adolescent; Female; Histocytochemistry; Humans; Immunochemistry; Lymphatic Diseases; Male; Microscopy, Electron; Middle Aged; Muramidase; Naphthol AS D Esterase; Protease Inhibitors; Skin Neoplasms

We investigated the effects of stromal cells, obtained from lymph nodes with various lymphoid disorders and characterized immunocytochemically as fibroblasts, on the maturation of a cell line "HN" established from an atypical PLL (prolymphocytic leukemia) having phenotypic characteristics of both CLL (chronic lymphocytic leukemia) and HCL (hairy cell leukemia). Coculture with stromal cells, irrespective of the kind of lymphoid disease affecting the lymph node, induced a plasmacytoid cytology in HN cells, an increase of cIg gamma, and decreases of sIg gamma, CD5, CD21, HC2, and B-ly7. In contrast, coculture with the stromal cells produced no marked effects on hairy cells from two HCL patients. Similarly, coculture with stromal cell supernatant or with various cytokines shown to be produced by bone marrow stromal cells produced no effects on HN cells. We propose that lymph node stromal cells play an important role in the differentiation of B cells through direct contact and that HN cells will be useful for investigating the differentiation pathway of chronic B cell leukemia cells.

Topics: Acid Phosphatase; Antigens, CD; B-Lymphocytes; Blood Coagulation Factors; Cell Differentiation; Cells, Cultured; Cytokines; Humans; Leukemia, Hairy Cell; Leukemia, Lymphocytic, Chronic, B-Cell; Leukemia, Prolymphocytic; Lymph Nodes; Lymphatic Diseases; Phenotype; Receptors, Antigen, B-Cell; Stromal Cells; Tumor Cells, Cultured

The morphological, enzyme- and immunohistochemical features of a sarcoma arising from interdigitating reticulum cells (IDRC) are presented. These cells are normal constituents of the T-dependent region of lymphoid organs, and their function is largely unresolved. The immunohistochemical findings in the present case indicate that neoplastic IDRC are morphologically and phenotypically similar to normal IDRC in lymphoid organs. Functionally however, neoplastic IDRC more closely resemble IDRC in the fetal lymph node and in the thymus, where they are thought to play a role in the final differentiation of immature thymocytes into mature peripheral T-lymphocytes.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Aged; Antibodies, Monoclonal; Female; Histocytochemistry; Humans; Immunoenzyme Techniques; Lymphatic Diseases; Lymphoid Tissue; Lymphoma, Large B-Cell, Diffuse; Microscopy, Electron

Two Chinese patients with sinus histiocytosis and massive lymphadenopathy are reported. The results of enzyme and immunohistochemical studies are presented.

Topics: Acid Phosphatase; Adult; alpha 1-Antitrypsin; Asian People; Biopsy; Child; China; Esterases; Female; Histiocytes; Humans; Immunoenzyme Techniques; Immunoglobulins; Lymph Nodes; Lymphatic Diseases; Male; Muramidase; Otitis Media with Effusion; S100 Proteins

Malignant histiocytosis is a rare neoplastic disease from the group of acute leukemias. Morphological characteristics of the disease are presented on the basis of histological, histochemical, and electron microscopic examinations of section materials from 16 cases of malignant histiocytosis in children of 6 to 14 years. Systemic focal-diffuse proliferation of tumor cells of histiocytic type of various degrees of differentiation was observed in organs and tissues. Most frequently, typical macro- and/or microscopic lesions were detected in lymph nodes, bone marrow of vertebrae, flat and tubular bones, liver, and spleen. The histiocytic nature of tumor cells was judged primarily from such signs as their capacity for phagocytosis detectable by light and electron microscopy, high diffuse cytoplasmic activity on nonspecific esterase and acid phosphatase, and features of the ultrastructure. In the establishment of the diagnosis, malignant histiocytosis had to be differentiated from Letters-Siwe disease, sinus histiocytosis with massive lymphadenopathy, lymphogranulomatosis, immunoblastic lymphosarcoma, melanoma, undifferentiated cancer.

Topics: Acid Phosphatase; Adipose Tissue; Adolescent; Child; Esterases; Female; Humans; Lymph Nodes; Lymphatic Diseases; Male; Microscopy, Electron

Eight cases of autopsy-proved malignant histiocytosis (MH) are reported, in which cutaneous involvement was a prominent finding at initial clinical presentation. In two patients, the disease remained confined to the skin for significant periods before systemic dissemination appeared. Immunohistochemical analysis of cutaneous infiltrates of MH showed uniform negativity of the atypical cells for lysozyme, immunoglobulin light chain, glycogen, and chloroacetate esterase content. In five cases, cytochemical stains for acid phosphatase, butyrate (nonspecific) esterase, chloroacetate esterase, and peroxidase activity were performed on bone marrow aspirates containing malignant cells; all demonstrated diffuse positivity for acid phosphatase and nonspecific esterase, and negativity for peroxidase and chloroacetate esterase. Touch-imprint preparations of cutaneous lesions in one of these cases yielded identical results. These findings indicate that cytochemical methods are preferable to immunohistochemical technics in identification of histiocytic malignancies and that foci of skin involvement may provide a useful source for such diagnostic evaluation.

Topics: Acid Phosphatase; Adult; Aged; Bone Marrow; Esterases; Female; Histocytochemistry; Humans; Liver; Lymphatic Diseases; Lymphoid Tissue; Male; Middle Aged; Skin; Skin Neoplasms

The synovium and synovial fluid have been studied in a patient with multicentric reticulohistiocytosis. Previously unreported histochemical, immunocytochemical and ultrahistochemical findings are presented and their relevance to the aetiology and pathogenesis are discussed. The synovial fluid analysis in this disease is characteristic and may be helpful in its early diagnosis.

Topics: Acid Phosphatase; Aged; Arylsulfatases; Humans; Joint Diseases; Leukocyte Count; Lymphatic Diseases; Male; Microscopy, Electron; Synovial Fluid; Synovial Membrane

Topics: Acid Phosphatase; Animals; Butyrates; Cell Differentiation; Clone Cells; Glucuronidase; Histiocytoma, Benign Fibrous; Humans; Lymphatic Diseases; Methylcholanthrene; Mice; Mice, Inbred BALB C; Neoplasms, Experimental; Rosette Formation

Regressing atypical histiocytosis (RAH) of skin is a cutaneous noduloulcerative proliferation of atypical neoplastic histiocytes with concomitant polymorphous inflammation, frequently pronounced epidermal hyperplasia, and an unexpectedly indolent biologic course. Spontaneous regression and recurrence without systemic spread were the course in follow-up periods of over six years. Histopathologically, characteristic-appearing atypical mononuclear and multinucleated "RAH" cells showed erythrophagocytosis as well as ultrastructural, surface marker, and enzyme cytochemical features indicating histiocytic differentiation. Cytogenetic analysis showed aneuploidy and several marker chromosomes including 14q+. Its benign biologic course clearly distinguished this entity from malignant histiocytosis, large cell lymphoma, and Hodgkin's disease. The histiocytic atypical cells further distinguished it from the T-cell lesions of the skin, such as mycosis fungoides and lymphomatoid papulosis. This entity is readily confused with malignant lymphoreticular disease, melanoma, or squamous carcinoma.

Topics: Acid Phosphatase; Cell Differentiation; Child; Chromosome Banding; Cytoplasm; Diagnosis, Differential; Follow-Up Studies; Histiocytes; Humans; Hyperplasia; Lymphatic Diseases; Male; Middle Aged; Neoplasm Recurrence, Local; Skin Neoplasms

So far, enzyme histochemical examination has been applied, with few exceptions, either to tissue imprints or to cryostat sections of freshly collected samples. This procedure is not easily applicable in routine histopathologic examination. In this study, a simplified tissue embedding procedure is presented which can be performed using an automatic tissue changer. The paraffin embedded samples can be used for both conventional histopathologic examination and for demonstrating enzymes in sections. The enzymes studied (alkaline phosphatase, alpha-naphthyl acetate esterase, acid phosphatase, tartrate resistant acid phosphatase, ATPase, peroxidase, and chloroacetate esterase) gave comparable results in formalin-fixed cryostat sections and paraffin sections in both normal and pathologic lymphoid samples. The only exception was ATPase, which could not be demonstrated on paraffin-embedded material. The technic described has broad application in the analysis of lymphoid diseases.

Topics: Acid Phosphatase; Alkaline Phosphatase; Histocytochemistry; Histological Techniques; Humans; Lymphatic Diseases; Lymphoid Tissue; Naphthol AS D Esterase; Paraffin; Specimen Handling

Topics: Acid Phosphatase; Animals; Cattle; Cattle Diseases; Convalescence; Diarrhea; Epithelium; Intestine, Small; Lymphatic Diseases; Lymphoid Tissue; Microscopy, Electron, Scanning; Microvilli; Peyer's Patches

Malignant histiocytosis with cutaneous involvement occurred in a 27-year-old man. There were generalized weakness, abdominal pain, lymphadenopathy, fever and firm, papulonodular cutaneous infiltrations. The tumour cells were identified as malignant histiocytes by specially adapted enzyme-cytochemical and immunocytological methods on cryostat sections and single-cell suspension from the cutaneous infiltrates. The malignant histiocytes are characterized by diffuse activity of esterases and acid phosphatase. In addition, tests in single-cell suspension make it possible to differentiate the cells further according to cytomorphological and enzyme-cytochemical criteria.

Topics: Acid Phosphatase; Adult; Esterases; Histiocytes; Humans; Lymphatic Diseases; Male; Skin Neoplasms

A case of the syndrome of sea-blue histiocyte is presented in a 53-year-old Japanese woman, which is the first recorded case in Japan. The patient had hepatosplenomegaly, bleeding manifestations, mild thrombocytopenia, fatty metamorphosis and cirrhosis of the liver, as well as abnormal serum lipid profiles. Her parents were consanguineous and her maternal grandmother with hepatomegaly died of hepatic failure. Histologically, peculiar histiocytes containing numerous, intracytoplasmic sea-blue stained granules on May-Giemsa stain were demonstrated in biopsy materials of the bone marrow, lymph node and liver. The sea-blue granules in these histiocytes proved to have histochemical staining characteristics of lipogenic ceroid-like pigment. Ultrastructurally, these granules showed membrane-bound, pleomorphic inclusions of heterogeneous nature, including electron-dense amorphous or variegatedly osmiophilic, frequently laminated materials. Enzyme cytochemically, localization of acid phosphatase activity was demonstrated in and around the intracytoplasmic inclusions. With regard to the pathogenesis of the sea-blue histiocytes in this case, it may be suggested that the existence of the abnormality in lipid metabolism plays an important role in intralysosomal ceroidogenesis in these histiocytes.

Topics: Acid Phosphatase; Bone Marrow; Female; Histiocytes; Histocytochemistry; Humans; Inclusion Bodies; Kupffer Cells; Liver; Lymphatic Diseases; Middle Aged; Syndrome; Triglycerides

A 25-year-old black female presented with lymphadenopathy, fever and anemia of two months duration. The diagnosis of malignant histiocytosis was made on the basis of histiocytic infiltrations in the sinuses of spleen, liver and lymph nodes and by the demonstration of erythrophagocytosis in bone marrow. Following splenectomy, the patient developed a leukemic phase with as many as 50 X 10(9) abnormal histiocytes/l and bone marrow necrosis. This patient was also atypical because of multiple granulomas in liver, spleen and lymph nodes. Cytochemical and immunofluorescent stains confirmed that the abnormal cells were derived from the monocyte-macrophage series. Electron microscopy was used to further characterize this abnormal cell population. The electron microscopic and cytochemical evidence confirms that the malignant cells in malignant histiocytosis are derived from monocytes.

Topics: Acid Phosphatase; Adult; Esterases; Female; Granuloma; Histocytochemistry; Humans; Lymphatic Diseases; Microscopy, Electron; Monocytes

The clinical, morphologic, and cytochemical findings of 7 patients with malignant histiocytosis are presented in this study. The diagnoses were confirmed by the use of electron microscopy and cytochemistry (acid phosphatase and naphthol-AS-acetate-esterase). Two different types of malignant histiocytes were identified by the differentiation and development of cell organells. The relation of malignant histiocytosis to monocytic leukemia and to normal histiocytes (and their precursors) is discussed. And finally, there are statements on the origin and peculiarities of malignant histiocytes.

Topics: Acid Phosphatase; Cell Differentiation; Cell Nucleus; Histiocytes; Humans; Leukemia, Myeloid; Lymphatic Diseases; Microscopy, Electron; Naphthol AS D Esterase; Organoids

Topics: Acid Phosphatase; Glucuronidase; Humans; Lymphatic Diseases; Lymphocytes; Lymphoma

A tartrate-resistant acid phosphatase was isolated from a human leukemic spleen by freeze-thawing in saline and purified by repeated chromatography on carboxymethyl-cellulose. The purified enzyme has a molecular weight of 64 000. It catalyzes the hydrolysis of inorganic and organic pyrophosphate as well as the phenolic ester of monoorthophosphate, with optimal activity between pH 5 and 6. However, there is no activity toward mono-orthophosphate esters of aliphatic alcohols. The present data have identified its catalytic function as a pyrophosphatase. However, it has properties different from the pyrophosphatase previously observed in normal animal tissues.

Topics: Acid Phosphatase; Binding, Competitive; Humans; Hydrogen-Ion Concentration; Kinetics; Lymphatic Diseases; Molecular Weight; Pyrophosphatases; Spleen; Splenic Neoplasms; Tartrates

Leukemic reticuloendotheliosis is a distinct entity, often misdiagnosed as chronic lymphocytic leukemia or lymphoma. The neoplastic cells have a specific tartrate-resistant acid phosphatase isoenzyme by which the diagnosis may be secured. Most individuals are leukopenic, and when, as in our case, rare or no circulating cells with tartrate-resistant acid phosphatase activity are found in the peripheral blood, an alternate site must be sought. Bone marrow aspirations often result in "dry taps," however, and cryostat sections of the bone marrow biopsy necessary to demonstrate tartrate-resistant acid phosphatase activity are involved and impractical to obtain for most laboratories. This report illustrates and recommends the simple technic of imprinting the core biopsy, which yields a satisfactory sample with which the specific cytochemical activity can be demonstrated.

Topics: Acid Phosphatase; Biopsy, Needle; Bone Marrow; Bone Marrow Cells; Humans; Isoenzymes; Leukemia; Lymphatic Diseases; Male; Middle Aged; Spleen; Splenomegaly; Staining and Labeling

The enzyme activity for alpha-naphthyl-acetate esterase, naphthol-AS-acetate esterase, naphthol-AS-D-chloroacetate esterase, acid phosphatase, L(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and Sézary syndrome. The neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and those of the so-called reticulum cell sarcoma had no characteristic nature of the reticulum cells or histiocytes enzyme histochemically. These findings suggest that these neoplastic cells may be derived from the B-cell system. In Sézary syndrome, acid phosphatase activity was localized in a small paranuclear area in Sézary cells, which were considered to have a T-cell nature. It is thought that these enzyme histochemical methods are easy and useful in differentiating the B- or T-cell nature and the classification of non-Hodgkin's lymphomas.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aged; Esterases; Female; Histocytochemistry; Humans; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Middle Aged; Naphthaleneacetic Acids; Naphthol AS D Esterase; Nucleotidases

Topics: Acid Phosphatase; B-Lymphocytes; Esterases; Humans; Lymphatic Diseases; Lymphoma; Mycosis Fungoides; Skin Neoplasms; Syndrome; T-Lymphocytes

Nonspecific esterase activity using alpha-naphthyl butyrate as substrate was found to be present in hairy cells from patients with hairy cell leukemia. Activity of this enzyme was markedly diminished and in some instances obliterated by sodium fluoride. Since alpha-naphthyl butyrate is believed to be a more specific substrate for monocytic-type nonspecific esterase than alpha-naphthyl acetate, its presence in hairy cells combined with fluoride inhibition which is also characteristic of monocytic nonspecific esterase provides additional evidence of monocytic properties of hairy cells.

Topics: Acetates; Acid Phosphatase; Bone Marrow; Butyrates; Esterases; Humans; Leukemia; Lymphatic Diseases; Monocytes; Sodium Fluoride

In vitro studies were performed with leukaemic cells from two patients with hairy cell leukaemia in order to define the nature and kinetics of immunoglobulin synthesis by the neoplastic cells. Both patients had clinically and morphologically well-defined disease and their cells contained abundant tartrate-resistant acid phosphatase. One patient had associated macroglobulinaemia. The hairy cells had B-lymphocyte characteristics as determined by fluorescent immunoglobulin staining and surface receptor properties. They synthesized monoclonal IgM and IgG respectively in vitro. The kinetics of immunoglobulin synthesis were different in cells from the two patients as measured by equilibration time, intracellular degradation, and secretion. Permanent cell lines were established with cells from these patients. The lines grow as typical B-lymphoblastoid cultures and continue to produce tartrate-resistant acid phosphatase and immunoglobulin. These studies unequivocally demonstrate the B-lymphocyte nature of the hairy cells in these patients and provide evidence for their clonal origin both in terms of immunoglobulin and enzyme synthesis.

Topics: Acid Phosphatase; Cell Line; Clone Cells; Humans; Immunoglobulin G; Immunoglobulin M; Leukemia; Lymphatic Diseases; Male; Microscopy, Electron; Microscopy, Electron, Scanning; Middle Aged

The tartrate-resistant acid phosphatase was isolated from the spleen of a patient with leukemic reticuloendotheliosis. The unique characteristic of the enzyme is its similar reactivity toward p-nitrophenylphosphate, inorganic pyrophosphate ADP, and ATP. When ATP was incubated with the enzyme, the initial products were ADP and inorganic phosphate. The kinetic data confirmed that these substrates were hydrolyzed by the same enzyme. This type of substrate specificity is clearly different from acid phosphatase and pyrophosphatases previously described in the literature.

Topics: Acid Phosphatase; Adenine Nucleotides; Binding Sites; Humans; Kinetics; Leukemia; Lymphatic Diseases; Nitrobenzenes; Spleen; Tartrates

Reports of negative tartrate-resistant acid phosphatase reactions in a few cases of leukemic reticuloendotheliosis prompted the authors to re-evaluate the diagnostic specificity of this test. As a result, they modified the test by (1) incorporating a dual-control system for excluding a false-negative test due to technical errors, and (2) instituting an objective grading system for assuring consistent interpretation of the test on blood smears. When these modifications were applied to materials of patients suspected to have leukemic reticuloendotheliosis, there was an excellent, although not specific, correlation between the positive test and the diagnosis of leukemic reticuloendotheliosis. Tartrate-resistant acid phosphatase reactions were positive, intermediate, and negative for 76, 21, and 3% of 29 patients who had leukemic reticuloendotheliosis, whereas the figures were 3, 32, and 65%, respectively, for 37 patients who had chronic lymphocytic leukemia and other hematologic disorders.

Topics: Acid Phosphatase; Diagnosis, Differential; False Negative Reactions; Histocytochemistry; Humans; Leukemia; Lymphatic Diseases; Tartrates

Topics: Acid Phosphatase; Adult; Basophils; Female; Humans; Isoenzymes; Leukemia; Lymphatic Diseases

A fresh spleen sample obtained from a patient with leukemic reticuloendotheliosis was homogenized and subjected to centrifugation on a sucrose density gradient. A major portion of acid phosphatase band 5 was observed in the lysosome, confirming that the elevated phosphatase activity in the neoplastic spleen is a lysosomal enzyme. However, a significant amount of brand 5 was also observed in the microsome. The microsomal and lysosomal enzymes have different affinity to CM-cellulose. The relationship between lysosomal and microsomal enzymes has not been established.

Topics: Acid Phosphatase; Humans; Leukemia; Leukocyte Count; Lymphatic Diseases; Male; Middle Aged; Spleen; Splenectomy

Topics: Acid Phosphatase; Adult; Aged; Blood Cells; Female; Humans; Leukemia; Lymphatic Diseases; Male; Middle Aged

Two months after presenting with autoimmune hemolytic anemia, a 57-year-old man developed increased serum acid phosphatase activity. Subsequently, abnormal histiocytes were found in the bone marrow and blood, and 8 months after the onset of his illness lymph node biopsy led to the diagnosis of histiocytic medullary reticulosis. At that time the level of serum acid phosphatase activity was more than 60 times the upper limit of normal and then paralleled the activity of his disease during temporary responses to chemotherapy, rising to over 90 times normal at the time of his death 1 year after presentation. No prostatic malignancy was demonstrated by biopsies or at autopsy, and electrophoretic studies of the serum enzyme suggested that it was of extraprostatic origin. It is postulated that the acid phosphate present in the serum at levels heretofore described only in metastatic carcinoma of the prostate may have arisen from the abnormal histiocytes.

Topics: Acid Phosphatase; Bone Marrow; Histiocytes; Humans; Lymph Nodes; Lymphatic Diseases; Male; Middle Aged; Neoplasm Metastasis; Prostatic Neoplasms

Topics: Acid Phosphatase; Ceroid; Child; Female; Histiocytes; Histocytochemistry; Humans; Lipids; Lymphatic Diseases; Splenomegaly; Staining and Labeling

On account of 2 own observations, main clinical and diagnostic features of Hairy cell leukemia (HCL) will be discussed. HCL is a rare, unusual type of chronic leukemia and is predominantly particular of middle-aged men. The occurrence of middle-sized lymphoid cells having a hairy cytoplasmic edge, and a tartrate-resistant acid PHOsphatase isoenzyme are the characteristic criteria of the HCL. The diffuse infiltration by hairy cells affecting primarily the spleen and bone marrow results in anaemia, granulocytopenia, thrombocytopenia and splenomegaly. Differential diagnosis have to be made in relation to other lymphatic leukemias, leukemic malignant lymphomas and monoclonal gammopathies as well as lymphotropic viral infections. Immunologic behaviour of hairy cells is like that of B-lymphocytes. Therefore, the term "leukemic reticuloendotheliosis" should no longer be applied.

Topics: Acid Phosphatase; Adult; Age Factors; Agranulocytosis; Anemia; B-Lymphocytes; Bone Marrow; Diagnosis, Differential; Humans; Leukemia; Leukemia, Myeloid; Lymphatic Diseases; Male; Middle Aged; Sex Factors; Spleen; Splenomegaly; Thrombocytopenia

Topics: Acid Phosphatase; Diagnosis, Differential; Female; Humans; Isoenzymes; Leukemia; Lymphatic Diseases; Middle Aged; Reticulocytes

Topics: Acid Phosphatase; Bone Marrow; Cell Nucleus; Dermatitis, Exfoliative; Glucuronidase; Humans; Immunoglobulins; Keratoderma, Palmoplantar; Lymph Nodes; Lymphatic Diseases; Microscopy, Phase-Contrast; Staining and Labeling; Syndrome; T-Lymphocytes

Morphological and immunological studies of four cases of leukaemic reticuloendotheliosis are reported. The findings indicate that leukaemic reticuloendotheliosis is a monoclonal B cell neoplasm in which the leukaemic cell expresses either Kappa or lambda light chain and delta heavy chain determinants. The similarity between this disease and chronic lymphocytic leukaemia is discussed.

Topics: Acid Phosphatase; B-Lymphocytes; Blood Cells; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Chromatin; Cytoplasm; Endoplasmic Reticulum; Epitopes; Erythrocytes; Humans; Inclusion Bodies; Leukemia; Leukemia, Myeloid; Leukocytes; Lymphatic Diseases; Lymphocyte Activation; Microscopy, Electron; Monocytes

Topics: Acid Phosphatase; Aged; Alkaline Phosphatase; Autopsy; Biopsy; Bone Marrow; Bone Marrow Examination; Esterases; Female; Histiocytes; Humans; Lymphatic Diseases; Microscopy, Phase-Contrast; Mitosis; Peroxidases; Thrombocytopenia

Topics: Acid Phosphatase; Cytoplasm; Dermatitis, Exfoliative; Glucuronidase; Humans; Keratoderma, Palmoplantar; Leukemia, Lymphoid; Leukocyte Count; Lymphatic Diseases; Lymphocytes; Syndrome

Topics: Acid Phosphatase; Child, Preschool; Glycogen; Humans; Infant; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Lipids; Lymphatic Diseases; Peroxidases

Topics: Acid Phosphatase; Blood Cell Count; Chlorambucil; Fluorescent Antibody Technique; Histocytochemistry; Humans; Hypergammaglobulinemia; Immunoglobulin D; Immunoglobulin E; Immunoglobulin M; Immunoglobulins; Lectins; Leukemia; Leukocytes; Lymphatic Diseases; Lymphocytes; Male; Microscopy, Electron; Middle Aged; Prothrombin Time; Splenomegaly; Technetium; Uric Acid

Topics: Acid Phosphatase; Adult; Aged; Autopsy; Bone Marrow Examination; Female; Follow-Up Studies; Histiocytes; Histocytochemistry; Humans; Leukemia; Liver; Lymph Nodes; Lymphatic Diseases; Lymphocytes; Male; Middle Aged; Mitosis; Monocytes; Spleen; Splenectomy; Splenomegaly

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Blood Cell Count; Bone Marrow; Cyclophosphamide; Cytoplasm; Female; Hepatomegaly; Histocytochemistry; Humans; Leukemia; Liver; Liver Function Tests; Lymph Nodes; Lymphatic Diseases; Male; Microscopy, Electron; Middle Aged; Prednisone; Reticulin; Ribosomes; Spleen; Splenectomy; Splenomegaly

Topics: Acid Phosphatase; Bone Marrow Cells; Cell Membrane; Esterases; Fluorides; Histiocytes; Humans; Immunoglobulins; Liver; Lymph Nodes; Lymphatic Diseases; Megakaryocytes; Microscopy, Electron; Microscopy, Phase-Contrast; Spleen; Staining and Labeling; Tartrates

Topics: Acid Phosphatase; Biopsy; Endoplasmic Reticulum; Female; Glycosaminoglycans; Histiocytes; Humans; Lipoproteins; Lymphatic Diseases; Microscopy, Electron; Middle Aged; Mitochondria

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Bile Ducts; Bilirubin; Cholestasis; Cholesterol; Dogs; Hepatic Veins; Liver; Liver Cirrhosis, Biliary; Lymphatic Diseases; Lymphatic System; Nucleotidases; Vena Cava, Superior

Topics: Acid Phosphatase; Adolescent; Bone Marrow; Gaucher Disease; Humans; Lymphatic Diseases; Male

The pathognomonic "hairy cell" of leukemic reticuloendotheliosis was studied for ultrastructural localization of tartrate-resistant acid phosphatase activity. The enzyme activity could not be demonstrated by Gomori's method, but it was readily demonstrated in the cytoplasmic vesicles and Golgi saccules of the hairy cell by substitution of naphthol AS-BI phosphoric acid for Na-beta-glycerophosphate of Gomori's medium. This apparent substrate specificity may be used as a marker for electron microscopic identification of the hairy cell, and may facilitate the study of histogenesis of leukemic reticuloendotheliosis.

Topics: Acid Phosphatase; Cytoplasm; Glycerophosphates; Histocytochemistry; Humans; Isoenzymes; Leukemia; Lymphatic Diseases; Methods; Naphthols; Phosphoric Acids; Staining and Labeling

Topics: Acid Phosphatase; Bone Marrow Cells; Esterases; Histocytochemistry; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Lymphatic Diseases; Macrophages; Monocytes; Mononuclear Phagocyte System; Phospholipids; Pigments, Biological

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Histiocytes; Histocytochemistry; Humans; Lymphatic Diseases; Male; Microscopy, Electron

Topics: Acid Phosphatase; Bone Marrow; Diagnosis, Differential; Hodgkin Disease; Humans; Isoenzymes; Liver; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Spleen

Topics: Acid Phosphatase; BCG Vaccine; Biopsy; Epithelial Cells; Esterases; Female; Follow-Up Studies; Histocytochemistry; Humans; Immunity, Cellular; Immunoglobulin A; Infant; Infant, Newborn; Lectins; Leukocytes; Lymph Nodes; Lymphatic Diseases; Macrophages; Microscopy, Electron; Phagocytosis; Tuberculin; Tuberculin Test

Topics: Acid Phosphatase; Animals; Antidepressive Agents; Diagnosis, Differential; Glucuronidase; Indoles; Iprindole; Lung Neoplasms; Lymphatic Diseases; Macrophages; Male; Microscopy, Electron; Phagocytosis; Pulmonary Alveoli; Pulmonary Fibrosis; Rats; Thorium Dioxide

Topics: Acid Phosphatase; Adult; Blood Cell Count; Blood Platelets; Blood Protein Electrophoresis; Bone Marrow Examination; Female; Hematocrit; Hemoglobinometry; Humans; Isoenzymes; Leukemia; Leukocyte Count; Lymphatic Diseases; Male; Middle Aged; Reticulocytes; Splenectomy; Splenic Neoplasms; Tartrates

Topics: Acid Phosphatase; Blood Cells; Clinical Enzyme Tests; Humans; Isoenzymes; Leukemia; Lymphatic Diseases; Tartrates

Topics: Acid Phosphatase; Chronic Disease; Clinical Enzyme Tests; Esterases; Hepatitis A; Histocytochemistry; Humans; Infectious Mononucleosis; Leukemia; Leukemia, Lymphoid; Lymphatic Diseases; Lymphatic System; Lymphocytes; Methods; Monocytes; Naphthols; Peroxidases; Reticulocytes; Staining and Labeling

Topics: Acid Phosphatase; Acute Disease; Adenosine Triphosphatases; Alkaline Phosphatase; Asparaginase; Bone Marrow; Bone Marrow Cells; Carcinoma; Depression, Chemical; Esterases; Hodgkin Disease; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukocyte Count; Leukocytes; Lupus Erythematosus, Discoid; Lymphatic Diseases; Multiple Myeloma; Peroxidases; Stimulation, Chemical

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Cells; Bone Marrow Examination; Esterases; Histocytochemistry; Humans; Lectins; Leukemia; Lymphatic Diseases; Lymphocyte Activation; Lymphocytes; Male; Microscopy, Electron; Microscopy, Electron, Scanning; Microscopy, Phase-Contrast; Middle Aged; Mononuclear Phagocyte System; Peroxidases

Topics: Acid Phosphatase; Diagnosis, Differential; Electrophoresis, Disc; Histocytochemistry; Humans; Isoenzymes; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukocytes; Lymphatic Diseases; Lymphocytes; Lymphoma, Non-Hodgkin; Tartrates

Topics: Acid Phosphatase; Humans; Isoenzymes; Lymphatic Diseases; Tartrates

Topics: Acid Phosphatase; Blood Platelets; Bone Marrow; Bone Marrow Cells; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Electrophoresis, Disc; Fluorides; Gaucher Disease; Histocytochemistry; Humans; Isoenzymes; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Lymphatic Diseases; Lymphocytes; Lymphoma, Non-Hodgkin; Molecular Weight; Monocytes; Nitrophenols; Polycythemia Vera; Staining and Labeling; Tartrates

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Bone Marrow Cells; Histocytochemistry; Humans; Karyotyping; Lymphatic Diseases; Male; Peroxidases; Reticulocytes

Topics: Acid Phosphatase; Animals; Cell Nucleus; Cytoplasm; Endoplasmic Reticulum; Fishes; Golgi Apparatus; Histiocytes; Jaw Neoplasms; Lymphatic Diseases; Microscopy, Electron; Mitochondria; Mouth Neoplasms; Neoplasms; Phagocytosis; Ribosomes

Topics: Acid Phosphatase; Blood Proteins; Enzymes; Glucuronidase; Humans; Lectins; Lymphatic Diseases; Lymphocytes; Lysosomes; Malate Dehydrogenase; Mitosis; Plant Extracts; Stimulation, Chemical

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia, Aplastic; Bone Marrow; Disease; Hemochromatosis; Histocytochemistry; Humans; Hypersplenism; Leukocyte Count; Lymphatic Diseases; Methods; Mononuclear Phagocyte System; Multiple Myeloma; Neutrophils; Staining and Labeling

Topics: Acid Phosphatase; Copper; Health; Lymphatic Diseases; Neoplasms; Phosphoric Monoester Hydrolases