acid-phosphatase and Low-Back-Pain

Epidemiological studies have shown a relatively strong association between occupational lower back pain (LBP) and long-term exposure to vibration. However, there is limited knowledge of the impact of vibration and sedentariness on bone metabolism of the lumbar vertebra and the mechanism of bone-derived LBP. The aim of this study was to investigate the effects of vibration in forced posture (a seated posture) on biochemical bone metabolism indices, and morphometric and mechanical properties of the lumbar vertebra, and provide a scientific theoretical basis for the mechanism of bone-derived LBP, serum levels of Ca(2+), (HPO4)(2-), tartrate-resistant acid phosphatase (TRAP), bone-specific alkaline phosphatase (BALP), and bone gla protein (BGP),the pathological changes and biomechanics of lumbar vertebra of New Zealand white rabbits were studied. The results demonstrate that both forced posture and vibration can cause pathological changes to the lumbar vertebra, which can result in bone-derived LBP, and vibration combined with a seated posture could cause further damage to bone metabolism. Serological changes can be used as early markers for clinical diagnosis of bone-derived LBP.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Biomechanical Phenomena; Calcium; Female; Isoenzymes; Low Back Pain; Lumbar Vertebrae; Male; Organ Specificity; Osteocalcin; Phosphoric Acids; Posture; Rabbits; Restraint, Physical; Tartrate-Resistant Acid Phosphatase; Vibration

Topics: Absorptiometry, Photon; Acid Phosphatase; Adult; Anti-Inflammatory Agents, Non-Steroidal; Bone Density; Chronic Kidney Disease-Mineral and Bone Disorder; Diagnosis, Differential; Female; Humans; Isoenzymes; Low Back Pain; Lumbar Vertebrae; Magnetic Resonance Imaging; Osteopetrosis; Physical Therapy Modalities; Tartrate-Resistant Acid Phosphatase

Osteolysis and hypercalcemia are observed in 5-15%, and 10%, respectively, of malignant lymphoma patients during their clinical course. However, both osteolysis and hypercalcemia are uncommon at onset of the disease. We encountered a 24-year-old male non-Hodgkin's lymphoma patient who had multiple osteolytic lesion from the onset of the disease and repeated episodes of hypercalcemia during the clinical course. The patient died with refractory disease. We studied the expression of chemokines which might affect bone resorption using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Increased expressions of MIP-1alpha, MIP-1beta and RANKL, which are osteoclast-activating factors, were observed in the RNA derived from the patient's lymphoma cells. The secretion of osteoclast-activating factors such as MIP-1alpha by the tumor cells (and/or bone marrow stromal cells) might be involved in the etiology of osteolysis and hypercalcemia in some malignant lymphoma cases.

Topics: Acid Phosphatase; Adult; Biopsy; Bone Marrow Cells; Bone Resorption; Carrier Proteins; Chemokine CCL3; Chemokine CCL4; Chemokines; Fatal Outcome; Humans; Hypercalcemia; Immunophenotyping; Isoenzymes; Low Back Pain; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Macrophage Inflammatory Proteins; Magnetic Resonance Imaging; Male; Membrane Glycoproteins; Osteoclasts; Osteolysis; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Reverse Transcriptase Polymerase Chain Reaction; Tartrate-Resistant Acid Phosphatase