acid-phosphatase and Leukoplakia--Oral

Smear impressions of human buccal mucosa keratotic and nonkeratotic zones in health were studied by histochemical methods. Differences in the cytochemical spectrum of biopolymers contained in nucleus-free scales and nucleus-containing cells were revealed. Cationic proteins, acid phosphatase, and nonspecific esterase detected in exfoliative epithelial cells are particularly interesting. Exfoliative cells of the buccal epithelium play an important role in its protective reactions.

Topics: Acid Phosphatase; Adult; Carboxylesterase; Carboxylic Ester Hydrolases; Epithelial Cells; Epithelium; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Leukoplakia, Oral; Mouth Mucosa; Proteins; Reference Values; Surface Properties

The activity and isoenzyme profile of lactate dehydrogenase (LDH), alkaline and acid phosphatase were studied in tumors of the tongue, cheek, oral floor, soft palate and palatine tonsils (n = 100), leukoplakia (n = 7) and in the oral mucosa at corresponding sites in healthy subjects (n = 66), to develop tests for early detection, monitoring and prognosis of oral cancer. Levels of alpha-amylase and acid phosphatase were measured in saliva and blood serum of patients with oral cancer and healthy subjects. The activity of LDH and alkaline and acid phosphatase in oral malignancies were 1.5-6 times that in normal mucosa, depending on tumor site. Changes in LDH isoenzyme profile consisted in an increased level of M-subunits (LDH-4 and 5) and a decreased concentration of N-subunits (LDH-1 and 2). With regards to acid phosphatase, an increase in the activity of its tartrate-inhibited fraction was observed. An increase in LDH and alkaline phosphatase activity was registered for oral precancer (leukoplakia), too, although it was less pronounced than in cancer. Changes in LDH isoenzyme profile matched those in cancer patients. A significant increase in the activity of alpha-amylase, acid phosphatase and the latter's tartrate-inhibited fraction was registered in saliva of oral cancer patients (86-96%). The credibility of enzyme activity measurement in saliva for evaluation of response and prognosis is discussed.

Topics: Acid Phosphatase; Alkaline Phosphatase; alpha-Amylases; Clinical Enzyme Tests; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leukoplakia, Oral; Mouth Mucosa; Mouth Neoplasms; Saliva

Topics: Acid Phosphatase; Adenosine Triphosphatases; Humans; Leukoplakia, Oral; Microscopy, Electron; Mouth Mucosa

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Histocytochemistry; Humans; Leukoplakia, Oral; Microscopy, Electron; Mouth Mucosa

Glycogen in the epithelium is interpreted as a sign of cell activity; its accumulation seems to take place gradually during cell migration. The oxido-reductase and hydrolytic enzyme-groups represent a sensitive indicator of changes in cell metabolism. The occurrence and intensity of glycogen, lactic and succinic dehydrogenases, NAD-cytochrome-c-reductase, alkaline and acid phosphatases were studied in 20 biopsy materials obtained from patients with keratotic buccal lesions. The results were compared with 20 biopsies from healthy oral mucosa. Variations were found in the localization and amount of glycogen, but no significant difference could be observed in the intensity of the oxido-reductase enzymes and alkaline phosphatase reactions. Acid phosphatase showed increased reactivity in most superficial layers of the keratinized epithelium.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Epithelium; Female; Glycogen; Humans; L-Lactate Dehydrogenase; Leukoplakia, Oral; Male; Middle Aged; Mouth; NADH Dehydrogenase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Cell Nucleus; Cytoplasm; Epithelial Cells; Epithelium; Female; Golgi Apparatus; Humans; Keratosis; Leukoplakia, Oral; Male; Microscopy, Electron; Middle Aged; Mitochondria; Mitosis; Polyribosomes; Ribosomes; Skin Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Esterases; Humans; Leukoplakia, Oral; Lip Neoplasms; Mouth Neoplasms; Oxidoreductases; Precancerous Conditions

Topics: Acid Phosphatase; Aged; Cheek; Endoplasmic Reticulum; Epithelium; Female; Golgi Apparatus; Humans; Leukoplakia; Leukoplakia, Oral; Lysosomes; Male; Microscopy, Electron; Middle Aged; Mouth Mucosa; Mouth Neoplasms