acid-phosphatase and Leukemia-P388

BDF1 mice treated with Corynebacterium parvum (C. parvum) 2 days before an implant of 106 P388 leukemic cells had up to an 110% increase in survival time above control; Bacillus Calmette-Guérin (BCG) treatment was ineffective. Acid phosphatase and beta-glucuronidase were measured in adherent peritoneal lavage cells and beta-glucuronidase in peritoneal lavage fluid form mice treated with C. parvum or BCG 2 days before the implant of P388 cells. In the presence of the tumor, adherent peritoneal cells from C. parvum-treated animals had a 250-300% increased specific lysosomal enzyme activity above control values (cells form animals receiving tumor implant alone). Peak enzyme activity which occurred on day 3 was not present in adherent cells from BCG-treated tumor-bearing animals or the control animals. The beta-glucuronidase activity in peritoneal lavage fluid was elevated by the tumor cells, BCG, or C. parvum. Peak levels occurred on day 5 regardless of the treatment with an additive effect present on day 5 in animals receiving the combination of tumor with C. parvum. The evidence indicated the development of a different pattern of enhanced lysosomal enzyme activity if the immunopotentiator protected against the P388 tumor vs one that did not. Protection was associated with an increase in lysosomal enzyme activity in adherent cells with no increase in lavage fluid in the presence of tumor cells. Changes in cellular enzyme activity may prove to be diagnostic for antitumor activity by an immunostimulant.

Topics: Acid Phosphatase; Animals; Ascitic Fluid; Bacterial Vaccines; Glucuronidase; Leukemia P388; Leukemia, Experimental; Lysosomes; Male; Mice; Propionibacterium acnes; Therapeutic Irrigation; Time Factors; Transplantation, Homologous

The number of tumor cells recovered form the peritoneal cavity of mice administered Corynebacterium parvum (C. parvum), Bacillus Calmette-Guérin (BCG) or saline 2 days before tumor implant was assessed on days 1, 2, 3, 5 and 7 following the intraperitoneal (ip) administration of 10(6) P388 leukemic cells. C. parvum-treated mice manifested a significant decrease in the number of tumor cells recovered from the peritoneal cavity on days 3-7, while BCG-treated mice had tumor cell yields comparable to saline control values. Lysosomal enzyme activity (acid phosphatase and beta-glucuronidase) in the nonadherent lavage cell population, which comprised tumor cells and host cells obtained form tumor-bearing animals, closely reflected the changes observed in tumor cell numbers and was initially assumed to be associated with tumor cells. When lysosomal enzyme activity was expressed as a function of the number of tumor cells or cellular protein, an enhanced activity following C. parvum treatment but not following BCG treatment was demonstrated. Enzyme activity associated with tumor cells was maximal 2 days prior to the profound depression in tumor cell maximal 2 days prior to the profound depression in tumor cell yield and may be associated with tumor cell killing. It is concluded that a correlation may exist between lysosomal enzyme activity, tumor cell numbers and the protective effect of the immunostimulant C. parvum. Whether the correlation is direct or indirect remains to be resolved.

Topics: Acid Phosphatase; Animals; Bacterial Vaccines; Cells, Cultured; Glucuronidase; Leukemia P388; Leukemia, Experimental; Lysosomes; Male; Mice; Propionibacterium acnes; Time Factors; Transplantation, Homologous