acid-phosphatase and Leukemia--Monocytic--Acute

Numerous isoenzymes are used as markers in the course of malignant haemopathies. These are notably the isoenzymes of lactic dehydrogenase, hexosaminidase, esterases, acid phosphatases, and thymidine kinase. Their study already permits a finer and more rigorous classification of leukaemias and makes it possible to entertain serious hopes in at least three spheres: a better choice of treatment, surveillance of therapeutic efficacy and of remissions, and the development of new modes of therapeutic action by means of selective inhibitors.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adenosine Deaminase; Alkaline Phosphatase; alpha-Galactosidase; alpha-Mannosidase; Aminopeptidases; B-Lymphocytes; Burkitt Lymphoma; Cell Differentiation; Cyclic AMP; Esterases; Hexokinase; Hexosaminidases; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Lysosomes; Mannosidases; Phosphofructokinase-1; Protein Kinases; Purine-Nucleoside Phosphorylase; Pyruvate Kinase; Ribose-Phosphate Pyrophosphokinase; T-Lymphocytes; Thymidine Kinase

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Bone Marrow Cells; Cerebral Hemorrhage; Daunorubicin; Female; Gastrointestinal Hemorrhage; Glucuronidase; Humans; Leukemia; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Male; Middle Aged; Monocytes; Muramidase; Naphthol AS D Esterase; Periodic Acid-Schiff Reaction; Peroxidases; Pregnancy; Prognosis; Remission, Spontaneous

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Bone Marrow; Bone Marrow Cells; Cytoplasmic Granules; Diagnosis, Differential; Eosinophils; Erythrocytes; Esterases; Hematopoietic Stem Cells; Histocytochemistry; Humans; Iron; Leukemia; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Monocytes; Multiple Myeloma; Naphthaleneacetic Acids; Neutrophils; Peroxidases; Plasma Cells; Skin Window Technique; Staining and Labeling

The discovery of osteoprotegerin (OPG), osteoprotegerin ligand (OPGL), and RANK has elucidated the mechanism by which osteoblasts and stromal cells regulate osteoclastic differentiation and function and mediate the effects exerted by other hormones and cytokines. We have investigated the effects of these novel cytokines on the preosteoclastic cell line FLG 29.1. We show that OPGL alone and in combination with macrophage colony-stimulating factor (CSF-1) dramatically reduced replication and increased tartrate-resistant acid phosphatase activity. However, although FLG29.1 cells appear to adhere to the bone surface, they are not able to form resorption lacunae. OPG and calcitonin completely abolished the differentiation induced by OPGL. RANK was detectable in FLG 29.1 and the number of positive cells was increased by OPGL/CSF-1 treatment while reduced by calcitonin. We propose that calcitonin could interact with the OPG/OPGL, and its effects on RANK may explain in part the action of this hormone in suppressing bone resorption.

Topics: Acid Phosphatase; Calcitonin; Carrier Proteins; Cell Adhesion; Cell Differentiation; Cell Division; Cell Survival; Cells, Cultured; Filaggrin Proteins; Glycoproteins; Humans; Isoenzymes; Leukemia, Monocytic, Acute; Lymphocytes; Macrophage Colony-Stimulating Factor; Membrane Glycoproteins; Osteoclasts; Osteoprotegerin; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Receptors, Cytoplasmic and Nuclear; Receptors, Tumor Necrosis Factor; Recombinant Proteins; Tartrate-Resistant Acid Phosphatase; Tumor Cells, Cultured

Cells of U937, a human monocytic leukemia cell line, differentiate into macrophages by treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA), whereas cells treated with 1 alpha, 25-dihydroxyvitamin D3 [1,25-(OH)2D3] continue to grow without undergoing differentiation. When U937 cells were successively treated with TPA and 1,25-(OH)2D3, tartrate-resistant acid phosphatase-positive multinucleated cells appeared at 5 days after the treatment. These osteoclast-like cells released a soluble form of 45Ca from 45Ca-labeled bone particles. These cells were not formed when the order of treatment with TPA and 1,25-(OH)2D3 was reversed. Use of either dexamethasone or interferon-gamma (IFN-gamma) was effective in inhibiting the formation of these osteoclast-like cells. The expression of c-src, c-fms, and macrophage colony stimulating factor (M-CSF) was induced by TPA treatment; however, TPA-induced M-CSF gene transcription was attenuated by the subsequent addition of 1,25-(OH)2D3. Furthermore, both dexamethasone and IFN-gamma impaired the attenuation of M-CSF expression, suggesting that the transient expression of M-CSF may be important for the formation of osteoclast-like cells.

Topics: Acid Phosphatase; Animals; Calcitonin; Calcitriol; Carcinogens; Cell Differentiation; Cell Nucleus; Dexamethasone; Diterpenes; Drug Synergism; Gene Expression Regulation, Leukemic; Genes, fms; Genes, src; Humans; Interferon-gamma; Isoenzymes; Leukemia, Monocytic, Acute; Macrophage Colony-Stimulating Factor; Mice; Osteoclasts; Phenotype; Tartrate-Resistant Acid Phosphatase; Terpenes; Tetradecanoylphorbol Acetate

Human permanent leukemia cell lines represent powerful research tools in a multitude of investigations. The two new continuous leukemia cell lines MUTZ-2 and MUTZ-3 were derived from the peripheral blood of patients with acute myeloid leukemia (AML) FAB M2 and AML FAB M4. MUTZ-2 and MUTZ-3 cells have morphological and immunophenotypical features of myeloid and monocytic cells, respectively. While MUTZ-2 is negative, MUTZ-3 cells express the monocytic surface marker CD14, albeit weakly. The monocytic nature of MUTZ-3 cells is underlined by the expression of the monocyte-specific esterase (MSE), myeloperoxidase (MPO) and tartrateresistant acid phosphatase (TRAP) enzymes; MUTZ-2 is negative for MSE and TRAP, but expresses MPO. For sustained cell growth, both cell lines require constitutively the addition of cytokines to the culture medium and retain an absolute dependence on conditioned medium or recombinant growth factors for proliferation and survival. Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ-2 cells was elicited by FLT-3 ligand, granulocyte colony-stimulating factor (G-CSF), macrophage CSF (M-CSF), interferon-gamma (IFN-gamma) and stem cell factor (SCF), whereas granulocyte-macrophage CSF (GM-CSF), M-CSF, interleukin-3 (IL-3) and SCF were the most effective growth factors in inducing proliferation of MUTZ-3. Both cell lines were proliferatively responsive to several further cytokines, however, to a lesser extent. Exposure to phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or the physiological all-trans retinoic acid (ATRA) had growth-inhibitory and differentiation-inducing effects on both cell lines. Using a clonogenic cell recovery assay, both cell lines were found to be sensitive to the chemotherapeutic drugs cytosine arabinoside (Ara-C) and daunorubicin (DNR), MUTZ-2 cells being more sensitive to both Ara-C and DNR treatment than MUTZ-3 cells. Chromosomal trisomies 8 and 10 were found in MUTZ-2 cells without any additional structural abnormalities. MUTZ-3 carries the rare, but recurrent AML-associated translocation (12;22)(p13;q11-q12) reflecting the karyotype of the original tumor. The main characteristics of these cell lines remained the same during about 1 year of continuous culture as well as after freezing and thawing. In summary, we established and characterized two new leukemia cell lines with myeloid or monocytic features which ar

Topics: Acid Phosphatase; Adult; Antineoplastic Agents; Base Sequence; Cell Differentiation; Cell Division; Chromosome Aberrations; Cytokines; Esterases; Hematopoietic Cell Growth Factors; Humans; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Male; Middle Aged; Molecular Sequence Data; Peroxidase; Tetradecanoylphorbol Acetate; Tretinoin; Tumor Cells, Cultured

Human monoblastoid cell line U-937 was adapted to grow in protein-free (protein-free hybridoma--PFH) medium and cloned by limiting dilution. Resulting cell subline (U-937/PF) cultured in protein-free medium was characterized by immunological, cytochemical and biochemical techniques. There were no major differences in immunophenotype (determined by FACS analysis with monoclonal antibodies directed to HLA and CD antigens) and cytochemical markers between the U-937/PF cells cultured in protein-free cell culture medium and parental U-937 cell cultured in serum-supplemented medium. Maximal cell density was slightly decreased in protein-free culture as compared to the parental cell line in FCS-supplemented medium. Cell viability and cell DNA histograms (determined by propidium iodide cytofluorimetry) showed no major differences between parental U-937 and U-937/PF cells. Phorbol ester (TPA)-induction of differentiation-associated cell markers resulted in a proliferation arrest and accumulation of G0/G1 cells in both sublines. All-trans retinoic acid and, to a lesser extent, TPA-stimulated NBT reduction was higher in parental U-937 cells cultured in serum-supplemented medium as compared to U-937/PF cells. Quantitative differences in the expression and inducibility of some cytochemical markers (beta-glucuronidase, chloroacetate esterase) were found between both examined sublines. Described U-937/PF subline cultured in a protein-free cell culture medium (PFH) appeared as a potential tool for studies of in vitro inducing agents and serum components with differentiation promoting (or inhibiting) activities.

Topics: Acid Phosphatase; Antigens, CD; Biomarkers, Tumor; Carboxylic Ester Hydrolases; Cell Cycle; Cell Line; DNA; Flow Cytometry; Fluorescent Antibody Technique; Glucuronidase; Histocompatibility Antigens Class I; HLA-DR Antigens; Humans; Immunophenotyping; Leukemia, Monocytic, Acute; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured

The human leukemia cell line CTV-1 was established from a case of acute monoblastic leukemia (AMoL). We analyzed the phenotypic marker profile of the CTV-1 cells in their original, untreated state and during induction of differentiation with the phorbolester 12-0-tetradecanoylphorbol 13-acetate (TPA). TPA led to morphological changes with signs of differentiation. Cell proliferation decreased in a dose-dependent fashion during exposure to TPA. In the surface marker analysis using a panel of 45 monoclonal antibodies (MoAbs) and several polyclonal antisera, CTV-1 cells were negative for markers of the T- and B-cell lineages, and were positive for several, but not all, myelomonocytic markers. Although the cells were reactive with the MoAb Leu-7 which identifies natural killer (NK) T-cells, no NK activity was detected. In the isoenzyme analysis of the four enzymes carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) performed by isoelectric focusing on polyacrylamide gels, CTV-1 cells displayed isoenzyme profiles of immature myeloid cells. The overall marker profile of CTV-1 cells demonstrated cells of monocytoid origin arrested at a very early stage of differentiation, possibly close to the stage of precursor cells. As compared to other myelomonocytic cell lines, CTV-1 cells showed unusual morphological, immunological, functional and biochemical features and appeared to be relatively insensitive to treatment with TPA, although some alterations of the phenotype could be induced.

Topics: Acid Phosphatase; Antigens, Surface; Carboxylic Ester Hydrolases; Cell Differentiation; Cell Division; Cell Line; Hexosaminidases; Humans; Isoenzymes; Killer Cells, Natural; L-Lactate Dehydrogenase; Leukemia, Monocytic, Acute; Tetradecanoylphorbol Acetate

This report describes the qualitative acid phosphatase (acP) isoenzyme profiles detected in permanent human hematopoietic cell lines. The acP activity was separated into its isoenzymes by isoelectric focusing on horizontal thin-layer polyacrylamide gels. The pattern of acP isoenzyme was investigated in a total of 86 cell lines. These cell lines were classified into five groups on the basis of their phenotypes characterized in the multiple marker analysis: 74 leukemia-lymphoma cell lines (26 T-, 34 B-, 6 myelomonocytic, 8 Non-T, Non-B cell lines) and 12 so-called 'normal' Epstein-Barr virus transformed B-lymphoblastoid cell lines. Their immunological features had been analysed in detail by use of a large panel of poly- and monoclonal antibodies which led to a further subclassification into stages of differentiation. A progressive increase in number and staining intensity of the isoenzymes which paralleled the expression of surface markers at different stages of differentiation along their developmental pathway was seen in the T- and B-leukemia-lymphoma cell lines. Some cell lines whose isoenzyme profiles did not correspond to the stage of differentiation as evidenced by surface antigen analysis might represent good examples of deranged gene expression in otherwise normally programmed malignant cells, i.e. in our study a mismatch between the isoenzymatic and immunological phenotypes. The tartrate-resistant isoenzyme was detected in 9 out of 74 leukemia-lymphoma cell lines (4 T-, 2 B-, 1 myelomonocytic, 2 Non-T, Non-B cell lines) and in 10 out of 12 normal B-lymphoblastoid cell lines; the only one studied hairy cell leukemia cell line did not express this isoenzyme. The relative specificity of the tartrate-resistant acP is discussed in detail. No leukemia-lymphoma specific isoenzyme or an additional isoenzyme which was not seen in normal hematopoietic cells could be observed. Nor did we find an isoenzyme or isoenzyme pattern characteristic for a certain cell lineage. This underlines the necessity of a combined analysis using markers from different disciplines in the 'multiple marker analysis' in order to accurately characterize normal and malignant blood cells. Furthermore, our results support the concept of maturation arrest at particular stages of differentiation together with the theory of normal gene expression in leukemic cells equivalent to that in their normal counterparts.

Topics: Acid Phosphatase; Antigens, Surface; B-Lymphocytes; Cell Differentiation; Cell Line; Humans; Isoenzymes; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Lymphoma; Phenotype; T-Lymphocytes; Tartrates

Differential diagnostic importance of acid phosphatase and beta-glucuronidase reactions was studied in bone marrow smears of 52 patients with acute leukaemias. Both reactions showed either diffuse or simultaneously diffuse and granular positivity in the medullary blast cells of 34 patients suffering from ANLL. A strong diffuse positivity of acid phosphatase suggested the possibility of AMOL. Beta-glucuronidase and acid phosphatase reactions were exclusively granular in every positive case of ALL. Increased acid phosphatase activity was found in T-ALL while beta-glucuronidase showed increased activity also in (non-T, non-B)-ALL on several occasions.

Topics: Acid Phosphatase; Acute Disease; Adolescent; Adult; Aged; Diagnosis, Differential; Glucuronidase; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Middle Aged; Receptors, Antigen, B-Cell; T-Lymphocytes

An ultrastructural study of blast cells showing either monocytic or granulocytic differentiation was carried out with the acid phosphatase (AP) and myeloperoxidase (MPO) reactions. Eight cases of acute myeloid leukaemia (AML) and three of chronic granulocytic leukaemia in blast crisis were studied. A hitherto unrecognized small lysosomal granule characterized by AP activity and lack of MPO was present in the majority of cells of all six monoblastic leukaemias. These granules ranged from 0.05 to 0.2 micron in size and were distributed throughout the cytoplasm, frequently at the periphery of the cells. A small proportion of monoblasts showed AP reactivity in the Golgi cisternae. Both AP and MPO were positive in the granules of promonocytes; however, MPO positive granules were predominant in late promonocytes. Larger granules (0.2--0.6 micron) with MPO reactivity were characteristic of myeloblasts. In only two out of four cases did these granules show AP positivity, suggesting that, in contrast to monoblasts, AP activity is a late feature of myeloblastic differentiation. This study shows that ultrastructural cytochemistry may be helpful in the recognition and classification of acute leukaemias by demonstrating the early differentiation features of monocytic and granulocytic precursors.

Topics: Acid Phosphatase; Cytoplasmic Granules; Histocytochemistry; Humans; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Microscopy, Electron; Peroxidase

Topics: Acid Phosphatase; Adolescent; Chediak-Higashi Syndrome; Cytoplasmic Granules; Female; Humans; Leukemia, Monocytic, Acute

Topics: Acid Phosphatase; Acute Disease; Antigens, Neoplasm; Cytotoxicity Tests, Immunologic; Esterases; Female; Histocytochemistry; Humans; Infant; Infant, Newborn; Leukemia, Monocytic, Acute; Phagocytosis; Scalp; Skin Neoplasms

The influence of cytostatic drugs (L-asparaginase, vincristine, 6-mercaptopurine, amethopterine, prednisone) on the activity of alkaline and acid phosphatase, alpha-naphtol-acetate esterase, the content of glycogen and lipids in leukocytes of peripheral blood in patients with acute leukemia was investigated. Under the influence of anti-leukemic drugs some cytochemical reactions typically changed in different forms of acute leukemia showed tendency to normalization being sometimes more distinctive than leukocytosis or even than white blood picture. In patients who did not show any improvement during the treatment the disturbances of cytochemical reactions intensified or, sometimes, remaining unchanged. The repetition of examination of cytochemical reactions changing distinctively in the chemotherapy may simplify the treatment control by better estimation of its efficiency and give some prognostic hints.

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Antineoplastic Agents; Female; Glycogen; Granulocytes; Humans; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Lipids; Lymphocytes; Male; Middle Aged; Naphthol AS D Esterase

The normal isoenzymatic pattern of leucocytic acid-phosphatase based on the study of 150 haematologically normal individuals is reported. The different pathologic patterns of the leucocytic acid-phosphatase isoenzymes occurring in non-lymphoblastic acute leukaemias are presented and correlated with the subdivisions of acute leukaemias established by the French-American-British (FAB) Co-operative Group. This study is considered to be especially useful in identifying pure acute monocytic leukaemias corresponding to subtype M5 of the FAB as well as acute erythraemias with unusual cytological and cytochemical features.

Topics: Acid Phosphatase; Electrophoresis, Cellulose Acetate; Humans; Isoenzymes; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocyte Count; Leukocytes

Topics: Acid Phosphatase; Evaluation Studies as Topic; Glycogen; Histocytochemistry; Humans; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Lipids; Lymphocytes; Prognosis

Using the high resolution technique of isoelectric focusing in polyacrylamide gel, isoenzymatic components of acid phosphatase were detected in cell-free extracts prepared from different cytologic types of leukemic blasts in adults. Results indicate that for different cytologic types, different characteristic patterns of acid phosphatase isoenzyme could be detected. These studies extend conventional cytochemistry and indicate that characteristic patterns of acid phosphatase isoenzyme can be detected for various cytologic types of acute leukemia.

Topics: Acid Phosphatase; Cell-Free System; Granulocytes; Humans; Isoelectric Focusing; Isoenzymes; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocytes; Lymphocytes; Monocytes; Veins

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Ceruloplasmin; Clinical Enzyme Tests; Diagnosis, Differential; Enzyme Activation; Female; Humans; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Male; Middle Aged

Topics: Acetates; Acid Phosphatase; Esterases; Glycogen; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Naphthol AS D Esterase; Peroxidases

During a large clinicopathologic study of acute nonlymphocytic leukemia (ANLL), ten patients were identified in whom the leukemic blasts demonstrated striking morphologic and cytochemical similarities and who seemed to form a specific subgroup of ANLL. The patients' leukemic blasts were studied in routine blood and bone marrow preparations and by cytochemical and ultrastructural techniques. In routine smears, the blasts showed no clear evidence of differentiation. Cytochemically, the blasts exhibited strongly positive nonspecific esterase activity, which was completely inhibited by incubation with sodium fluoride, and were myeloperoxidase and sudan black B negative. Ultrastructural features of the blasts were similar to those described for monocytic leukemias. Striking clinical features included the occurrence primarily in young patients, the high frequency of lymphadenopathy at presentation, and the high incidence of post-treatment disseminated intravascular coagulation. Complete remissions were frequently initially obtained with duanorubicin in combination with various other agents and later in the disease with VP16-213. Based on the cytochemical and ultrastructural features, we concluded that this form of ANLL was a variety of acute monocytic leukemia. Recognition of the entity is important for optimal therapy.

Topics: Acid Phosphatase; Adolescent; Adult; Bone Marrow; Child; Child, Preschool; Cytarabine; Daunorubicin; Esterases; Female; Histocytochemistry; Humans; Leukemia, Monocytic, Acute; Male; Methotrexate; Methyl Green; Periodic Acid; Peroxidases; Prednisone; Vincristine

Over 20 cytoenzymochemical tests were carried out in 152 patients with different types of acute leukemia to estimate the effects of some antiblastic drugs such as L-asparaginase, Purinethol, Methotrexate, Endoxan, Vinchristine, Cytosine Arabinoside a.o. The patients selected for the study were carefully examined before treatment at different moments during and/or at the end of the treatment. The effects of these drugs on the blast cells were mild when the cellular populations had a low rate of nucleic acid synthesis, high glycogenic score and high amounts of lipids or an important oxidative enzymatic activity. The enzymatic prediction tests: the acid phosphate deviation test and the succinic dehydrogenase inhibition test including the variant suggested by some of the authors - the latic dehydrogenase inhibition test - gave satisfactory results only in certain cases of acute leukemia.

Topics: Acid Phosphatase; Antineoplastic Agents; DNA; L-Lactate Dehydrogenase; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocytes; Prognosis; RNA; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adult; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Child; Cytoplasmic Granules; Histocytochemistry; Humans; Leukemia; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Microscopy, Electron; Monocytes; Neutrophils; Peroxidases; RNA, Neoplasm

Topics: Acid Phosphatase; Adult; Esterases; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocytes; Lymphocytes; Microscopy, Electron; Mitochondria; Monocytes; Peroxidases

Topics: Acid Phosphatase; Adult; Aged; Bone Marrow Examination; Esterases; Female; Histocytochemistry; Humans; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocyte Count; Leukocytes; Male; Microscopy, Electron; Muramidase; Skin Window Technique

Topics: Acid Phosphatase; Adult; Aged; Autopsy; Biopsy; Bone Marrow Cells; Cytoplasm; Cytoplasmic Granules; Female; Glycosaminoglycans; Golgi Apparatus; Histocytochemistry; Humans; Leukemia, Monocytic, Acute; Male; Microscopy, Electron; Middle Aged; Mitochondria; Monocytes; Neutrophils; Peroxidases

Topics: Acid Phosphatase; Alkaline Phosphatase; Amidohydrolases; Binding Sites; Culture Techniques; Diagnosis, Differential; Esterases; Female; Histocytochemistry; Humans; Immunoglobulin G; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Male; Microscopy, Electron; Monocytes; Muramidase; Oxidoreductases; Peroxidases; Skin Window Technique

Topics: Acid Phosphatase; Diagnosis, Differential; Electrophoresis, Disc; Histocytochemistry; Humans; Isoenzymes; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukocytes; Lymphatic Diseases; Lymphocytes; Lymphoma, Non-Hodgkin; Tartrates

Topics: Acid Phosphatase; Esterases; Histocytochemistry; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Periodic Acid; Peroxidases; Staining and Labeling

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Marrow; Bone Marrow Cells; Cytoplasmic Granules; Esterases; Glucosyltransferases; Histocytochemistry; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Periodic Acid; Staining and Labeling

Topics: Acetates; Acid Phosphatase; Aged; Alkaline Phosphatase; Anhydrides; Anilides; Bone Marrow Cells; Cytoplasmic Granules; Eosinophils; Esterases; Female; Humans; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Periodic Acid; Peroxidases

Topics: Acetates; Acid Phosphatase; Amidohydrolases; Culture Techniques; Diagnosis, Differential; Esterases; Humans; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Macrophages; Monocytes; Naphthalenes; Staining and Labeling

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Marrow; Esterases; Histocytochemistry; Humans; Leukemia; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Monocytes; Research; Tissue Culture Techniques