acid-phosphatase and Leukemia--Erythroblastic--Acute

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Bone Marrow; Bone Marrow Cells; Cytoplasmic Granules; Diagnosis, Differential; Eosinophils; Erythrocytes; Esterases; Hematopoietic Stem Cells; Histocytochemistry; Humans; Iron; Leukemia; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Monocytes; Multiple Myeloma; Naphthaleneacetic Acids; Neutrophils; Peroxidases; Plasma Cells; Skin Window Technique; Staining and Labeling

The activities of acid and alkaline phosphatases and phosphotyrosine, phosphoserine and phosphothreonine phosphatases were measured in Friend murine erythroleukaemic (MEL) cells. The effects of treating the cells with dimethyl sulphoxide (DMSO), an inducer of differentiation, were examined. In untreated cells alkaline phosphatase activity was undetectable, though there were significant amounts of acid phosphatase (76 +/- 15 mU/mg protein) and phosphotyrosine phosphatase (16 +/- 0.9mU/mg protein); phosphoserine and phosphothreonine phosphatase activities (9 +/- 0.4 and 7 +/- 0.6mU/mg protein, respectively) were lower than for phosphotyrosine phosphatase. Addition of 1 or 2% DMSO to the culture medium resulted in the expected cell death within 2 weeks. With 0.5% DMSO, cells remained viable for at least 8 weeks, but while some appeared to have smaller nuclei and retained their rounded appearance, others became fibroblastic within several days and adhered to the culture vessel. The treated cells which had kept their morphology showed no difference in acid phosphatase activities as compared with untreated controls; phosphotyrosine phosphatase was lower (9 +/- 0.8mU/mg protein) and phosphoserine and phosphothreonine phophatases higher (11 +/- 0.5 and 10 +/- 0.4mU/mg protein, respectively) than in the controls. The Km values for p-nitrophenyl phosphate were similar in untreated and treated cells (0.069 and 0.068mM, respectively); for phosphotyrosine the Km value was lower in the treated cells (0.97mM) than in the controls (1.9mM).

Topics: Acid Phosphatase; Animals; Cell Line; Cell Survival; Dimethyl Sulfoxide; Friend murine leukemia virus; Kinetics; Leukemia, Erythroblastic, Acute; Mice; Phosphoprotein Phosphatases; Phosphoric Monoester Hydrolases; Protein Tyrosine Phosphatases

We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-beta-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all controls (mean 9.4% of erythroblasts). The percentage of positive erythroblasts in pathological conditions varied greatly from absence to over 70%. Acid phosphatase and N-acetyl-beta-glucosaminidase were positive in pernicious anaemia and in acute erythremic myelosis. Nonspecific esterase was only detected in a case of erythremic myelosis. This case also showed a faint metachromasia with the dye azure A.

Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaline Phosphatase; Anemia, Aplastic; Erythroblasts; Esterases; Humans; Leukemia, Erythroblastic, Acute

We investigated the effect of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the human erythroleukemia cell line, HEL, and found that TPA addition (10(-6)-10(-8) M) to HEL cell cultures induces morphological, functional, and biochemical changes in HEL cells that are characteristic for macrophage-like cells. Apart from the drastic changes in morphology, the cells greatly enhance their phagocytic ability and acquire receptors for binding and degradation of chemically modified lipoproteins. At the biochemical level, a newly synthesized 85K glycoprotein is observed, and the cells are unresponsive to inducers of globin synthesis. Comparative observations with K562 cells indicate that TPA inhibits, as in HEL cells, spontaneous and induced globin synthesis, but induces minimal macrophage-like properties in these cells. The results with HEL cells are interpreted to indicate that TPA uncovers a latent monocyte-like phenotype in these cells.

Topics: Acid Phosphatase; Antigens, Surface; Cell Adhesion; Cell Line; Erythrocytes; Esterases; Globins; Glycoproteins; Humans; Leukemia, Erythroblastic, Acute; Macrophages; Phagocytosis; Phenotype; Phorbols; Receptors, Cell Surface; Receptors, LDL; Tetradecanoylphorbol Acetate

Topics: Acid Phosphatase; Acute Disease; Child, Preschool; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukocytes; Male

Topics: Acetates; Acid Phosphatase; Esterases; Glycogen; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Naphthol AS D Esterase; Peroxidases

Cytochemical, biochemical and disc-elektrophoretical studies are done on a patient with Di Guglielmo-syndrom. Cytochemical tests showed the wellknown results as: activity of acid phosphatase and alpha-naphthylacetatesterase at their typical sites, deposition of PAS- and Turnbull-positive material. Biochemical findings are done on LDH, cholinesterase, alkaline and acid phosphatase, G-6-PDH and pyruvatkinase. Abnormal high level of fetal hemoglobin was found only once during the whole observation. There was also an alteration in the isoenzym pattern of the acid phosphatase. The de- or increase at the biochemical examinations, the fact of fetal hemoglobin and the variation at the isoenzymes may be due to the changing in distribution of erythropoetic and granulopoetic cells.

Topics: Acid Phosphatase; Cholinesterases; Cytarabine; Esterases; Fetal Hemoglobin; Humans; Leukemia, Erythroblastic, Acute; Male; Middle Aged; Naphthaleneacetic Acids

Topics: Acid Phosphatase; Adult; Antibiotics, Antineoplastic; Asparaginase; Blood Transfusion; Bone Marrow Examination; Daunorubicin; Drug Combinations; Esterases; Glucuronidase; Hematologic Diseases; Humans; Leukemia; Leukemia, Erythroblastic, Acute; Male; Megakaryocytes; Methotrexate; Peroxidases; Prednisolone; Spleen; Staining and Labeling; Thrombocytopenia; Vincristine

Topics: Acid Phosphatase; Adult; Aged; Chromatin; Cytoplasm; Esterases; Female; Ferritins; Hematopoietic Stem Cells; Hemosiderin; Humans; Leukemia, Erythroblastic, Acute; Male; Microscopy, Electron; Middle Aged; Mitochondria; Naphthaleneacetic Acids

Topics: Acid Phosphatase; Aminosalicylic Acids; Anemia, Pernicious; Bone Marrow; Bone Marrow Cells; Diagnosis, Differential; Esterases; Histocytochemistry; Humans; Iron; Leukemia, Erythroblastic, Acute; Leukemia, Myeloid, Acute; Staining and Labeling

Topics: Acid Phosphatase; Adult; Aged; Bone Marrow Examination; Chromosome Aberrations; Chromosomes, Human, 6-12 and X; Diagnosis, Differential; Erythrocytes; Esterases; Female; Histocytochemistry; Humans; Iron; Leukemia, Erythroblastic, Acute; Periodic Acid

Topics: Acid Phosphatase; Adult; Esterases; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Leukocytes; Lymphocytes; Microscopy, Electron; Mitochondria; Monocytes; Peroxidases

Topics: Acid Phosphatase; Acute Disease; Antineoplastic Agents; Blood Cell Count; Bone Marrow; Bone Marrow Examination; Child, Preschool; Cytodiagnosis; Diagnosis, Differential; Erythrocytes; Erythropoiesis; Esterases; Histocytochemistry; Humans; Immunosuppressive Agents; Indicators and Reagents; Infant; Leukemia, Erythroblastic, Acute

Topics: Acid Phosphatase; Diagnosis, Differential; Erythrocytes; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Polycythemia Vera; Staining and Labeling

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia, Macrocytic; Bone Marrow Examination; DNA; Erythrocytes; Erythropoiesis; Esterases; Histocytochemistry; Humans; Indicators and Reagents; Leukemia; Leukemia, Erythroblastic, Acute; Periodic Acid; Spectrophotometry

Topics: Acid Phosphatase; Adolescent; Age Factors; Bone Marrow Examination; Child; Cyclophosphamide; Cytarabine; Diagnosis, Differential; Erythema; Erythrocytes; Humans; Leukemia, Erythroblastic, Acute; Male; Mercaptopurine; Myelography

Topics: Acid Phosphatase; Bone Marrow Cells; Child; Clinical Enzyme Tests; Diagnosis, Differential; Erythropoiesis; Esterases; Histocytochemistry; Humans; Leukemia; Leukemia, Erythroblastic, Acute; Leukocyte Count; Pathology; Polycythemia Vera

Topics: Acid Phosphatase; Bone Marrow; Bone Marrow Cells; Esterases; Histocytochemistry; Humans; In Vitro Techniques; Leukemia, Erythroblastic, Acute; Polycythemia Vera