acid-phosphatase and Leiomyoma

Uterine leiomyomata and histologically normal myometrium were examined by light and electron microscopy. All myometrial samples contained smooth muscle cells with distinctive cytoplasmic inclusions located at the nuclear poles. These were seen in only one case in routine paraffin wax sections, where they appeared as clear vacuoles, but they were more obvious in epoxy resin sections stained with toluidine blue. Staining with Oil Red O and for acid phosphatase was positive at the site of the vacuoles. In ultrathin sections, these structures possessed a single membrane and contained abundant lipid droplets, with a sparse, coarsely granular matrix. They were designated as lipid-rich residual bodies. Such organelles were all but absent from leiomyomata. Lipid-rich residual bodies with the features described here have not been studied in detail before in smooth muscle cells of the human myometrium. The possibility that they arise from the cyclical stimulation of lipid synthesis by ovarian hormones is discussed.

Topics: Acid Phosphatase; Adult; Aged; Azo Compounds; Female; Humans; Leiomyoma; Lipids; Menorrhagia; Middle Aged; Myometrium; Organelles; Uterine Neoplasms

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Female; Histocytochemistry; Humans; Leiomyoma; Placenta; Pregnancy; Pregnancy Complications, Neoplastic; Uterine Neoplasms

In 132 patients suffering from various malignancies, including uterine carcinoma, breast cancer, gastric carcinoma, cancer of the colon, and additionally, patients with uterine myomas and endometriosis an increased activity of AP within the peripheral blood neutrophils could be stated by using a histochemical method. According to the author's opinion an increased activity of that enzyme reflects a response against local inflammatory processes frequently accompanying malignant tumours.

Topics: Acid Phosphatase; Breast Neoplasms; Colonic Neoplasms; Endometriosis; Female; Humans; Leiomyoma; Male; Neoplasms; Neutrophils; Stomach Neoplasms; Uterine Neoplasms

Enzymatic equipment in peripheral blood neutrophils has been determined in 10 women with cervical carcinoma, 5 with carcinoma of the uterus body and 30 women with myomas of the uterus. Using cytochemical techniques the authors observed the intracellular deficiency of N-acetyl-beta-glucosaminidase accompanied by diminished absolute count of the enzyme-positive cells as well as the beta-glucuronidase- and the myeloperoxidase-positive neutrophils. Lowered activity of the myeloperoxidase was another pattern of cells in question. The acid phosphatase activity of neutrophils in the patients was significantly elevated. It has been suggested that observed intracellular deficiencies of selected enzymes within the neutrophils are of importance with regard to lowered antitumor activity of that cells operating mainly through the myeloperoxidase-H2O2-halide system.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Female; Glucuronidase; Glycogen; Humans; Leiomyoma; Middle Aged; Neutrophils; Peroxidase; Uterine Cervical Neoplasms; Uterine Neoplasms

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Alkaline Phosphatase; Aminopeptidases; Bronchial Neoplasms; Carcinoma, Adenoid Cystic; Carcinoma, Bronchogenic; Clinical Enzyme Tests; Diagnosis, Differential; Dihydrolipoamide Dehydrogenase; Esterases; Glucosephosphate Dehydrogenase; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Hamartoma; Histocytochemistry; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Leiomyoma; Lung Neoplasms; Malate Dehydrogenase; Nucleotidases; Phosphogluconate Dehydrogenase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Androgens; Biopsy; Collagen; Esterases; Female; Glycosaminoglycans; Gonadotropins, Pituitary; Humans; Hydroxysteroid Dehydrogenases; Hypothalamus; L-Lactate Dehydrogenase; Leiomyoma; Microscopy, Electron; Ovarian Neoplasms; Ovary; Polycystic Ovary Syndrome; Uterine Neoplasms

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Dihydrolipoamide Dehydrogenase; Esterases; Histocytochemistry; Humans; Hydrolases; Leiomyoma; Leiomyomatosis; Neoplasms; Oxidoreductases; Phosphorylases; Phosphotransferases; Skin Neoplasms; Transaminases