acid-phosphatase and Laryngeal-Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Biomarkers, Tumor; Carcinoma, Squamous Cell; Female; Humans; Isoenzymes; Laryngeal Diseases; Laryngeal Neoplasms; Male; Middle Aged; Osteoclasts; Osteolysis; Tartrates

The relationship between cartilage and invading neoplastic cells was studied in 32 cases of laryngeal cancer by histological and cytochemical methods. Cartilage invasion was present in 12 cases, 10 of which were in proximity or in contact with areas of calcification and ossification. It was significantly correlated only to tobacco consumption (P less than .05) and, in regard to glottic tumors, to tumor diameter greater than 3 cm (P less than .01). Histologically, neoplastic invasion in cartilage was massive in 2 cases, occurred in areas of ossification in 4, between cartilage and bone in 4, and in epiglottic cartilage in 2. In 3 of the cases with bone invasion, there was also new bone formation. Hyaline cartilage and bone resorption was due to tartrate-resistant acid phosphatase (TRAP)-positive giant cells; in epiglottic cartilage only mononuclear cells were present, some of which were TRAP-positive. These results show that neoplastic cells can promote not only resorption and formation of bone, but also resorption of cartilage, which is considered resistant to neoplastic invasion. The different types of resorbing cells in contact with hyaline cartilage and bone in laryngeal cancer, and elastic cartilage in epiglottic cancer, suggest that the structure of the tissue being resorbed can influence the type of resorbing cells.

Topics: Acid Phosphatase; Biomarkers, Tumor; Carcinoma, Squamous Cell; Cell Nucleolus; Cell Nucleus; Chromatin; Cytoplasm; Cytoplasmic Granules; Epiglottis; Female; Histocytochemistry; Humans; Laryngeal Cartilages; Laryngeal Neoplasms; Leukocytes, Mononuclear; Male; Neoplasm Invasiveness; Tartrates; Thyroid Cartilage

Patients with various malignancies are characterized by decreased numbers of intact acid phosphatase-positive lysosomes within the peripheral blood lymphocytes as compared to the control groups of healthy subjects.

Topics: Acid Phosphatase; Adult; Aged; Female; Humans; Laryngeal Neoplasms; Lymphocytes; Lysosomes; Male; Middle Aged; Neoplasms; Precancerous Conditions

After in vitro incubation of human squamous cell carcinomas of the head and neck region with aromatic retinoid an increased number of lysosomes can be observed in the tumor cells. It is discussed whether this accumulaion of lysosomes is due to a direct stimulation of lysosomal enzyme synthesis or whether it is consequence of cell damage by the retinoid.

Topics: Acid Phosphatase; Acitretin; Carcinoma, Squamous Cell; Culture Techniques; Dose-Response Relationship, Drug; Humans; Laryngeal Neoplasms; Lysosomes; Mouth Neoplasms; Tretinoin

The occurrence, distribution, morphology and enzymatic activity of lysosomes in malignant keratinocytes of laryngeal carcinoma was studied by electron microscopy and ultrastructural cytochemistry. Lysosomes can be observed in various forms: dense bodies, multivesicular bodies, myelin bodies and residual bodies. The acid phosphatase activity varied both with regard to intensity and staining pattern. Specific reaction product in lysosomes is present in the cell cortex and in cytoplasmic processes of basal carcinoma cells. Extra-cellular localization of acid phosphatase was also observed at the tumor-stroma junction. The enzyme cytochemical and morphological results are discussed in relations to current concepts of lysosomal enzymatic activity in tumor cells. The findings suggest, that extra-cellular secretion of lysosomal enzymes may play an important role in facilitating tumor invasion and metastasis.

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Humans; Laryngeal Neoplasms; Laryngectomy; Lysosomes

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Carcinoma; Glucuronidase; Humans; Immunoglobulins; Laryngeal Neoplasms; Lymphocytes; Male; Middle Aged

The subcellular distribution of acid phosphatase in malignant keratinocytes of invasive laryngeal carcinoma was studied by ultrastructureal cytochemistry. The reaction product was localized in Golgi, ER, and some cytoplasmic vesicles. Acid phosphatase activity was also observed in lysosomal structures in the cortical cytoplasm of basal carcinoma cells. Extracellular acid phohphatase activity also occurred at the tumor-stroma junction in membrane-bound vesicular structures. The localization of acid phosphatase in this report is discussed in relation to acid phosphatase activity in other tumors. The findings lend further support to the important role of hydrolytic enzyme release in respect to tumor invasion into surrounding tissues.

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Humans; Laryngeal Neoplasms; Laryngectomy; Larynx; Lysosomes

In 30 patients with cancer of the larynx, aged 40 to 70 years, treated by radiotherapy 6 to 9 years ago the decreased activity of neutrophil beta-glucuronidase and N-acetyl-beta-glucosaminidase accompanied by a decrease of absolute count of enzyme-positive cells was noted. Numbers of acid phosphatase-positive neutrophils were also decreased. Moderate increase of the neutrophil alkaline phosphatase activity and of numbers of enzyme-positive cells was another observed feature. The main finding in lymphocytes of the patients consisted in the appearance of cells exhibiting diffusion of lysosomal enzymes, especially of beta-glucuronidase, N-acetyl-beta-glucosaminidase and to a lesser degree of acid phosphatase into the cell cytoplasm. Moderate increase of immunoglobulin level, especially that of IgA, reflected probably the immunologic mobilization of patients.

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Glucuronidase; Hexosaminidases; Humans; Immunoglobulins; Laryngeal Neoplasms; Lymphocytes; Male; Middle Aged; Neutrophils; Peroxidase

In patients with precancerous states and cancer of the larynx prior to and after radiotherapy exhibit the decreased activity of neutrophil beta-glucuronidase. Moreover patients treated by radiotherapy before the age of 6 to 9 years demonstrate deficiency of N-acetyl-beta-glucosaminidase in the above cells. The main finding in lymphocytes of the patients studied was in the appearance by diffusion of the above enzymes and of acid phosphatase in the cytoplasm, reflecting their release from lysosomes and immunological mobilization of these cells. The authors discuss the possible role of neutrophil enzymatic deficiency in lowering the antitumour cytotoxic effect of these cells.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Glucuronidase; Humans; Laryngeal Neoplasms; Leukocyte Count; Lymphocytes; Lysosomes; Male; Middle Aged; Neutrophils; Precancerous Conditions

Activity of beta-glucuronidase (GR) and acid phosphatase (AP) has been determined in peripheral blood neutrophils from 24 men with precancerous states of the larynx that is leukoplakia papillomas and pachydermia by means cytochemical methods described by Hayashi et al., and Barka and Anderson, respectively. The results obtained were expressed in terms of absolute counts of enzyme-positive and enzyme-negative cells with regard to enzyme activity variation within the enzyme-positive neutrophil population; the enzyme activity index score has been calculated. The control group consisted of 20 healthy subjects of the same sex. No significant alterations were found so far as AP activity is concerned between the group studied. In contrast, activity of GR in patients with precancerous states exhibited significant lowering. The most striking feature was in almost complete absence from the blood of GR-positive neutrophils with high activity of the enzyme. Majority of these cells showed only traces of the GR activity. According to authors opinion the deficiency of GR in neutrophils of patients with precancerous lesions pertains to problem of neutrophil-mediated cytotoxic effect against mammalian tumour cells.

Topics: Acid Phosphatase; Adult; Glucuronidase; Humans; Laryngeal Neoplasms; Male; Middle Aged; Neutrophils; Precancerous Conditions

In 24 men with precancerous states of the larynx, i.e. leukoplakia, papillomas and pachydermia, the peripheral blood lymphocytes were cytochemically stained for N-acetyl-beta-glucosaminidase, beta-glucuronidase, acid phosphatase and glycogen (PAS reaction). The results were expressed in terms of the absolute counts of enzyme- (or compound-) positive cells. The serum immunoglobulin IgG, IgA and IgM levels were also determined by Mancini's method. The results obtained were compared with those in 20 healthy men aged 20 to 30 years. It was found that the patients exhibited elevated numbers of N-acetyl-beta-glucosaminidase- and beta-glucuronidase-positive lymphocytes. A characteristic feature was an increase in the absolute counts of lymphocytes with diffuse and granular-diffuse types of cytochemical reaction for all enzymes studied. The number of cells with the granular type of enzymatic reaction (intact enzyme-positive lysosomes) was significantly diminished. These cytochemical alterations were accompanied by a significant increase in the serum IgA level. These results are discussed with reference to the lymphoid system response to tissues with precancerous lesions of the larynx.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Glucuronidase; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Laryngeal Neoplasms; Lymphocytes; Male; Middle Aged; Precancerous Conditions

Topics: Acid Phosphatase; Adult; Humans; Laryngeal Neoplasms; Lymphocytes; Lysosomes; Male; Middle Aged

Topics: Acid Phosphatase; Adult; Glucuronidase; Humans; Laryngeal Neoplasms; Lymphocytes; Lysosomes; Male; Middle Aged; Precancerous Conditions

In 20 men, aged 35 to 55 years, with untreated cancer of the larynx activity of lysosomal acid phosphatase (AP), beta-glucuronidase (GR) and N-acetyl-beta-glucosaminidase was determined cytochemically in peripheral blood lymphocytes and neutrophils by means of Barka and Anderson, Hayashi et al. and Hayashi's method, respectively; the results obtained were compared with those in 20 healthy men aged 20 to 30 years. Total count of GR-positive lymphocytes was higher in the patients than in normal persons. Total counts of AP-, GR-, and GS-positive lymphocytes with not disrupted enzyme-positive lysosomal granules within the cell cytoplasm were significantly lower and total counts of cells exhibiting the disruption of lysosomal granules and the diffuse type of cytochemical reaction were significantly higher in the patients when compared with the control group. The response of neutrophils consisted of a significant elevation in numbers of AP-, and GS-positive cells; overall score of enzyme activity studied in neutrophils was not altered in the patients. The authors disucss the significance of their observations in the light of data on participation of lymphocytic and neutrophilic lysosomal apparatus in the immunological response against tumour specific antigen in patients with cancer.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Carcinoma, Squamous Cell; Glucuronidase; Humans; Laryngeal Neoplasms; Lymphocytes; Lysosomes; Male; Middle Aged; Neutrophils

Topics: Acid Phosphatase; Adult; Glucuronidase; Humans; Hydrolases; Laryngeal Neoplasms; Lymphocytes; Male; Middle Aged; Neutrophils

The authors studied the modifications of the activities of some enzymes in cell cultures submitted to the action of biliverdin. This biliary pigment rapidly induces a remarkable increase in alkaline phosphatase and ATP-ase activities and subsequently, an activation of acid phosphatase and beta-glucuronidase. On the contrary, 5'-nucleotidase and glucose-6-phosphatase activities remain unchanged. These results are discussed and compared with those obtained in our and other laboratories by using unconjugated bilirubin on different biological substrates.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bilirubin; Carcinoma, Squamous Cell; Cell Line; Cell Membrane; Cell Nucleus; Cells, Cultured; Glucose-6-Phosphatase; Glucuronidase; Histocytochemistry; Humans; Laryngeal Neoplasms; Lysosomes; Mitochondria; Nucleotidases; Stimulation, Chemical

Topics: Acid Phosphatase; Glycosaminoglycans; Histocytochemistry; Humans; Laryngeal Neoplasms; Pharyngeal Neoplasms; Polysaccharides; Trachea

Topics: Acid Phosphatase; Adult; Carcinoma, Squamous Cell; Chronic Disease; Histocytochemistry; Humans; Hyperplasia; Laryngeal Diseases; Laryngeal Neoplasms; Laryngoscopy; Leukoplakia; Papilloma; Phosphoric Monoester Hydrolases; Precancerous Conditions

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Cell Line; Cell Membrane; Cytoplasm; Histocytochemistry; Laryngeal Neoplasms; Lysosomes; Microscopy, Electron; Poliovirus; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Glucuronidase; Glycosaminoglycans; Hexosaminidases; Histocytochemistry; Humans; Laryngeal Neoplasms; Polyps

Topics: Acid Phosphatase; Adenoviridae; Carcinoma; Cell Line; Cells, Cultured; Cytopathogenic Effect, Viral; Dimethyl Sulfoxide; Enzyme Activation; Humans; Hydrocortisone; Laryngeal Neoplasms; Lysosomes; Newcastle disease virus; Poliovirus; Spectrophotometry; Vaccinia virus; Vitamin A

Topics: Acid Phosphatase; Animals; Autolysis; Carcinoma; Cell Line; Cell Membrane; Chloroquine; Complement System Proteins; Culture Techniques; Dogs; Humans; Hydrocortisone; Immune Sera; Kidney; Laryngeal Neoplasms; Lysosomes; Microscopy, Phase-Contrast; Time Factors; Vitamin A

A correlation has been made between the host lysosomal responses to and release of infectious virus from HEp-2 cells infected with two strains of herpes simplex virus (HSV). Supravital staining with acridine orange was used for morphological studies of macroplaque and microplaque HSV-infected cells. With the progression of infection, cells infected with either microplaque HSV or macroplaque HSV were observed to undergo different lysosomal and cytopathic changes, which could be correlated with increased accumulation of acid phosphatase and infectious virus in the extracellular fluid.

Topics: Acid Phosphatase; Acridines; Carcinoma; Cell Line; Culture Techniques; Cytopathogenic Effect, Viral; Humans; Laryngeal Neoplasms; Lysosomes; Microscopy, Fluorescence; Microscopy, Phase-Contrast; Simplexvirus; Staining and Labeling

Topics: Acid Phosphatase; Adenocarcinoma; Biopsy; Carcinoma, Bronchogenic; Carcinoma, Squamous Cell; Colorimetry; Esophageal Neoplasms; Female; Gastrointestinal Neoplasms; Hodgkin Disease; Humans; Intestinal Neoplasms; Laryngeal Neoplasms; Leucyl Aminopeptidase; Leukemia; Liver Neoplasms; Lymphoma, Non-Hodgkin; Male; Melanoma; Nasopharyngeal Neoplasms; Neoplasms; Pancreatic Neoplasms; Prostatic Neoplasms; Tongue Neoplasms; Urogenital Neoplasms

Topics: Acid Phosphatase; Carcinoma; Cell Line; Chromosome Aberrations; Culture Techniques; Deoxyribonucleases; Guanidines; Humans; Karyotyping; Laryngeal Neoplasms; Lysosomes; Mitosis; Poliovirus; Virus Replication

Topics: Acid Phosphatase; Animals; Carcinoma; Cell Fractionation; Cell Line; Cells, Cultured; Centrifugation, Density Gradient; Connective Tissue; Culture Techniques; Fibroblasts; Galactosidases; Glucuronidase; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Laryngeal Neoplasms; Liver; Lysosomes; Male; Mice; Microscopy, Electron; Mitochondria; Proteins; Rats; Succinate Dehydrogenase; Sucrose; Sulfatases

Topics: Acid Phosphatase; Adolescent; Adult; Child; Child, Preschool; Esterases; Fibroma; Histocytochemistry; Humans; Infant; Karyometry; Laryngeal Neoplasms; Microscopy, Electron; Middle Aged; Pharyngeal Neoplasms