acid-phosphatase and Kidney-Tubular-Necrosis--Acute

Repeated intraperitoneal injections of ferric nitrilotriacetate (Fe-NTA) induce nephrotoxic features such as proximal tubular necrosis and renal failure, an unexpected phenomenon for a ferric compound. The mechanism of Fe-NTA toxicity was investigated by electron microscopy and respiration studies of renal cortical mitochondria in rats. Four hours after a single intraperitoneal injection of Fe-NTA, 5 mg iron/kg body wt, loss of microvilli, increased number of cytoplasmic vacuoles, electron-dense cytoplasmic deposits, mitochondrial swelling, karyorrhexis, and rupture of cytoplasmic membrane were observed in proximal tubular epithelia. At 24 hours, an increased number of cells had become necrotic. Polarographic studies of mitochondria from renal cortex 4 hours after Fe-NTA treatment showed a significant decrease in State 3 respiration and DNP-uncoupled respiration, whereas little change was observed in State 4 respiration and ADP/O.

Topics: Acid Phosphatase; Acute Kidney Injury; Animals; Epithelium; Ferric Compounds; Iron; Kidney; Kidney Cortex; Kidney Tubular Necrosis, Acute; Kidney Tubules, Proximal; Male; Microscopy, Electron; Mitochondria; Nitrilotriacetic Acid; Oxygen Consumption; Rats; Rats, Inbred Strains; Succinate Dehydrogenase

The activity of several enzymes with different intracellular sites was determined in urine at various times following nonfatal acute tubular necrosis induced by mercuric chloride administration. The excretion rate of all tested enzymes rose on the 1st and 2nd day; in the next observations (days 7-15) enzymatic values approached the basal values. The lactate dehydrogenase isoenzyme pattern of the renal cortical zone showed an early shift towards cathodic fractions and later (7 days) an increase of middle ones; the normal anodic zymogram recovered after a suitable time interval (30 days). The isoenzymatic changes are related both to the renal hypoxia and to the appearance of less differentiated cells. The behaviour of functional parameters (urine flow, osmolality, urea clearance, creatinine clearance) were well in agreement with the observed enzyme and renal isoenzyme changes.

Topics: Acid Phosphatase; Acute Kidney Injury; Alkaline Phosphatase; Animals; gamma-Glutamyltransferase; Glutamate Dehydrogenase; Isoenzymes; Kidney; Kidney Tubular Necrosis, Acute; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Male; Mercuric Chloride; Mercury; Rats

Topics: Acid Phosphatase; Acute Kidney Injury; Alkaline Phosphatase; Animals; beta-Aminoethyl Isothiourea; Calcinosis; Depression, Chemical; Electron Transport Complex IV; Histocytochemistry; Injections, Intraperitoneal; Kidney; Kidney Tubular Necrosis, Acute; Kidney Tubules, Proximal; Loop of Henle; Male; Nephrosclerosis; Nephrosis; Rats; Rats, Inbred Strains; Succinate Dehydrogenase; Time Factors

Topics: Acid Phosphatase; Acute Kidney Injury; Alkaline Phosphatase; Animals; Dextrans; Glucosephosphate Dehydrogenase; Glycerol; Histocytochemistry; Kanamycin; Kidney Tubular Necrosis, Acute; Kidney Tubules, Proximal; Male; Mercury; Rats; Succinate Dehydrogenase