acid-phosphatase and Keratosis

The 5'-nucleotidase activity in psoriatic and normal human epidermis was studied in comparison to acid phosphatase activity. The optimum pH in normal human epidermis was about 5.0 at room temperature. The activity of both enzymes was found to be high in the transitional zone. Acid phosphatase (non-specific) activity was strongly positive in the psoriatic parakeratotic horny layers whereas 5'-nucleotidase activity in that area was completely absent. The results suggest that the enzyme which degrades nucleoside-5'-phosphate to nucleoside and inorganic phosphate is not acid phosphatase but 5'-nucleotidase. Nuclear preservation in psoriatic hyperkeratosis was attributed to absence or inactivation of specific enzymes of nuclear degradation, such as 5'-nucleotidase, rather than acid phosphatase.

Topics: Acid Phosphatase; Adult; Epidermis; Humans; Hydrogen-Ion Concentration; Keratosis; Nucleotidases; Parakeratosis; Psoriasis; Skin

An investigation was made of the enzymatic, histochemical, and histopathologic characteristics of clinically healthy edentulous mucosae of non-denture wearers. The effects of dentures on the oral mucosa were examined and related to the length of time that dentures were worn. Succinodehydrogenase, acid phosphatase, and alkaline phosphatase enzymes were used along with periodic acid Schiff's stain. Hematoxylin and eosin stain was used for the histopathologic study. The results indicated that a denture stimulates the underlying mucosa for the first 3 years. A healthy mucosa results, showing slight hyperkeratinization and increased enzymatic activity. After 3 years there are atrophic changes which can lead to inflammatory changes. There is also a reduction in enzyme activity associated with wearing dentures for more than 3 years. Remaking the dentures after 3 years increases the enzymatic activity of the alveolar mucosa.

Topics: Acid Phosphatase; Acrylic Resins; Adult; Alkaline Phosphatase; Denture, Complete; Epithelium; Female; Humans; Keratosis; Male; Middle Aged; Mouth Mucosa; Mouth, Edentulous; Periodontium; Succinate Dehydrogenase; Time Factors

Aminopeptidase and phosphatase activity of denture stomatitis mucosa was studied histochemically and biochemically. Stomatitis clearly increased the activity of these enzymes. An enzyme resembling the chloride-activated aminopeptidase B, usually involved in inflammatory processes, was recognized in the inflamed tissue in higher concentrations than in the control tissue.

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Aminopeptidases; Arginine; Denture, Complete, Upper; Female; Humans; Isoelectric Focusing; Keratosis; Male; Middle Aged; Mouth Mucosa; Palate; Phosphoric Monoester Hydrolases; Stomatitis; Stomatitis, Denture

Skin and oral lesions of chronic discoid lupus erythematosus from 6 patients have been investigated histologically and histochemically. Intra-individual comparisons between skin and oral lesions and intrasection comparisons between clinically affected and unaffected regions were performed. Enzyme histochemical recordings consisted of oxido-reductase and hydrolase activities. A high degree of correlation was noted in the intra-individual comparisons. The intrasection comparisons provided information concerning metabolic dynamics of the disease. The data obtained support the view that serious vascular changes are involved in the development of the changes in the overlying epithelium. The enzyme histochemical results agreed generally with histological data and proved to be a valuable diagnostic aid.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Basement Membrane; Cell Movement; Chronic Disease; Glucosephosphate Dehydrogenase; Glutamate Dehydrogenase; Histocytochemistry; Humans; Hydro-Lyases; Keratosis; Leucyl Aminopeptidase; Lupus Erythematosus, Discoid; Lymphocytes; Mouth Mucosa; NADH, NADPH Oxidoreductases; Oxidoreductases; Skin

Topics: Acid Phosphatase; Animals; Esterases; Histocytochemistry; Keratins; Keratosis; Male; Mouth Diseases; Mouth Mucosa; Muramidase; Rats; Sulfhydryl Compounds; Sulfides; Vitamin A Deficiency

Topics: Acid Phosphatase; Animals; Cheek; Deficiency Diseases; Enzyme Activation; Epithelial Cells; Epithelium; Histocytochemistry; Keratosis; Male; Mouth Mucosa; Naphthols; Phosphates; Rats; Zinc

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Cell Nucleus; Cytoplasm; Epithelial Cells; Epithelium; Female; Golgi Apparatus; Humans; Keratosis; Leukoplakia, Oral; Male; Microscopy, Electron; Middle Aged; Mitochondria; Mitosis; Polyribosomes; Ribosomes; Skin Neoplasms

Topics: Acid Phosphatase; Autoradiography; Biopsy; Dihydrolipoamide Dehydrogenase; Esterases; Humans; Keratosis; L-Lactate Dehydrogenase; Pityriasis; Proline; Skin; Succinate Dehydrogenase; Thymidine

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Biopsy; Child; Cholinesterases; Dermatitis, Exfoliative; Esterases; Female; Histocytochemistry; Humans; Ichthyosis; Keratosis; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Male; Microscopy, Electron; Middle Aged; NAD; Nucleosides; Oxidoreductases; Phosphoric Monoester Hydrolases; Skin; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Autoradiography; Cytoplasm; Electron Transport Complex IV; Esterases; Female; Glucosephosphate Dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; Hyalin; Hypertrophy; Ichthyosis; Isocitrate Dehydrogenase; Keratosis; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Microscopy, Electron; Nevus, Pigmented; Skin; Skin Diseases; Staining and Labeling; Succinate Dehydrogenase; Tritium; Uridine

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Alkaline Phosphatase; Autoradiography; Biopsy; Catechol Oxidase; Child, Preschool; Dihydroxyphenylalanine; Erythema; Esterases; Female; Glucuronidase; Histocytochemistry; Humans; Indicators and Reagents; Keratosis; Leucyl Aminopeptidase; Male; Nucleotidases; Oxidoreductases; Skin Temperature; Tritium

Topics: Acid Phosphatase; Alkaline Phosphatase; Humans; Hydrogen Peroxide; Keratosis; Occlusive Dressings; Periodontitis; Succinate Dehydrogenase

Topics: Acid Phosphatase; Foot Dermatoses; Hand Dermatoses; Humans; Keratosis; Lipid Metabolism; Peptide Hydrolases; Phospholipids; Skin