acid-phosphatase and Intestinal-Obstruction

Topics: Acid Phosphatase; Aged; Cell Membrane Permeability; Deoxyribonucleases; Erythrocyte Membrane; Female; Humans; Intestinal Obstruction; Male; Middle Aged; Peroxidases; Ribonucleases

Topics: Acid Phosphatase; Acute Disease; Animals; Deoxyribonucleases; Intestinal Obstruction; Intestine, Small; Liver; Lysosomes; Male; Rats; Ribonucleases

1. Complete mechanical occlusion of the ileum was produced in dogs and the loops above and below the obstruction were examined functionally and morphologically 4 or 7 days later. 2. The intraluminal pressure in the occluded loop never exceeded 8 cm water. 3. The mucosa above the obstruction secreted water and ions into the lumen in vivo, though it absorbed glucose normally. Mucosal slices also absorbed amino acids and monosaccharides normally in vitro. 4. The mucosa below the obstruction absorbed ions and glucose in vivo and non-electrolytes in vitro to a slightly smaller extent than normal intestine. 5. The morphological changes above the occlusion included shorter, plumper villi and shorter crypts, a reduction in histochemically stainable brush-border enzymes, but an increase in acid phosphatase. Below the obstruction, there was atrophy of the villi and crypts and reductions in all enzymes studied. 6. The results suggest that the mucosa above the occlusion possesses an intact and almost normal epithelial layer, but that it has been stimulated to secrete in vivo, presumably by intraluminal factors. Below the obstruction, true atrophy of the mucosa has promptly developed.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Calcium; Chlorides; Dogs; Female; Glucose; Ileum; Intestinal Mucosa; Intestinal Obstruction; Lactates; Leucyl Aminopeptidase; Magnesium; Male; Methylglucosides; Oxygen Consumption; Potassium; Sodium

Morphohistochemical and autoradiographic techniques were used to study the reactivity of the large intestine tunica mascularis under experimental ileus. The smooth muscle cells are subjected to pronounced changes. At early terms (1-2 days) reactive-compensatory reactions are observed. They include increased metabolic processes, hypertrophy of myocytes, their increased synthetic and proliferative activity. Simultaneously there appear degenerative phonomena which become predominant on the 4th-6th days. The level of metabolism in the smooth muscle tissue drops and the synthetic and proliferative activity of myocytes decreases. They undergo atrophy and lyse.

Topics: Acid Phosphatase; Animals; DNA; Glycosaminoglycans; Histocytochemistry; Intestinal Obstruction; Intestine, Large; Muscle, Smooth; Rats

A mechanical obstruction was produced in the dog ileum, and four days later, loop of intestine above and below the occlusion were subjected to one hour's ischaemia. This led to widespread morphological and functional damage of the epithelium, indistinguishable from the response of the normal intestine. The recovery of the mucosa of both loops after the ischaemia followed exactly the same pattern as when there was no occlusion superimposed: The transport capacity for phenylalanine in vitro was entirely restored, whereas that of beta-methyl-glucoside, together with oxygen consumption and lactate production, remained depressed; morphometric examination revealed that the recuperating mucosa had smaller villi, shorter crypts and smaller epithelial cells than the contiguous untouched tissue. These results suggest that the regenerative potential of the crypts could not be obliterated by one hour's ischaemia, either in the presence of a noxious obstruction fluid above the occlusion, or in the atrophic mucosa below it.

Topics: Acid Phosphatase; Animals; Dogs; Ileum; Intestinal Absorption; Intestinal Mucosa; Intestinal Obstruction; Ischemia; Lactates; Methylglucosides; Oxygen Consumption; Phenylalanine; Regeneration

After inducing experimental ileus in albino rats, the authors found changed lysosome enzyme activities in liver homogenizate. In the same kind "free" activity of acid phosphatase and acid ribonuclease is elevated by strangulation ileus. According to literature, these alterations result from changed permeability of lysosome membranes, resp. from rupture of lysosomes. Ileus by obstruction causes no significant changes of the "free" lysosomes activities in liver homogenizate. Increase of acid phosphatase and ribonuclease in blood serum by strangulation or obstruction is equally considerable in both kinds of ileus. The results of these experiments suggest the developing of hepatic damage under both kinds of experimental ileus, the extent of which can be assessed by determination of lysosome enzyme activities.

Topics: Acid Phosphatase; Animals; Disease Models, Animal; Endonucleases; Intestinal Obstruction; Lysosomes; Rats; Ribonucleases

Topics: Acid Phosphatase; Adrenal Glands; Adult; Alkaline Phosphatase; Animals; Dogs; Esterases; Female; Glycosaminoglycans; Histocytochemistry; Humans; Intestinal Obstruction; Intestine, Small; Kidney; Liver; Liver Glycogen; Male; Middle Aged; Myocardium; Necrosis; Pancreas; RNA; Succinate Dehydrogenase; Tissue Adhesions

Topics: Acid Phosphatase; Alkaline Phosphatase; Ammonia; Amylases; Animals; Body Fluids; Cathepsins; Creatine Kinase; Dogs; Fructose-Bisphosphate Aldolase; Glucuronidase; Intestinal Obstruction; Intestine, Small; Ischemia; L-Lactate Dehydrogenase; Lactates; Leucyl Aminopeptidase; Sulfatases