acid-phosphatase and Infertility

Semen samples of fertile and infertile men were analysed by gas liquid chromatography (GLC) for the presence of dichlorodiphenyltrichloroethane (DDT) and its metabolites (1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene) (pp'-DDE); (1,1,1-trichloro-2,2-bis(p-chlorophenyl ethane) (pp'-DDT); (1,1-dichloro-2,2 bis(p-chlorophenyl) ethane) (pp'-DDD); (1,1,1-trichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl) ethane) (op'-DDT), hexachlorocyclohexane (HCH) and its isomers alpha (alpha), beta (beta), gamma (gamma), delta (delta) and aldrin. The biochemical analysis of seminal vesicle and prostatic marker was done by spectrophotometer. The concentrations of alpha-, beta-, gamma-HCH, pp'-DDE and pp'-DDD were higher in the semen of infertile than in that of fertile men. An elevation in the fructose level along with decrease in the gamma-glutamyl transpeptidase and acid phosphatase activity was noticed in infertile men as compared to fertile subjects. The data showed correlation between alpha-, beta-, gamma-HCH, pp'-DDE, pp'-DDD and seminal vesicle marker fructose and prostatic marker gamma-gluatamyl transpeptidase and acid phosphatase in infertile men. The study suggests that the chlorinated pesticides may influence the semen quality by affecting the seminal and prostatic functions in infertile men.

Topics: Acid Phosphatase; Adult; Biomarkers; Chromatography, Gas; Fructose; Humans; Hydrocarbons, Chlorinated; Infertility; Male; Pesticides; Prostate; Semen; Seminal Vesicles

The study describes the concentrations of zinc, magnesium, copper, iron, and biochemical markers of seminal vesicle and prostate in seminal plasma of different categories of infertile men. The zinc level in oligasthenospermic subjects was significantly higher than in azoospermic, asthenospermic, and oligospermic subjects. The gamma-glutamyl transpeptidase activity in both oligoasthenospermic and azoospermic subjects were significantly lower than in asthenospermic and oligospermic individuals. There was no significant difference in iron, copper, and magnesium levels and acid phosphatase activity among the different infertile categories. A positive correlation between zinc and acid phosphatase (r=0.84, r= 0.73; p < 0.05), and between copper and fructose (r=0.81, r=0.72; p<0.05) was observed in oligoasthenospermic and azoospermic men, respectively. On the basis of our results, it may be postulated that there is a direct effect of the trace elements zinc and copper on acid phosphatase and fructose, respectively, in oligoasthenospermic and azoospermic subjects.

Topics: Acid Phosphatase; Adult; Biomarkers; Fructose; gamma-Glutamyltransferase; Humans; Infertility; Male; Semen; Trace Elements

Postcoital tests (PCTs) were conducted following artificial insemination with either live or dead spermatozoa. The cervical mucus samples were analyzed for sperm counts and acid phosphatase activity. The results indicate that the final distribution of mucus and sperm in the PCT sampling catheter is not truly representative of their original distribution within the cervix. Moreover, PCTs done after insemination with semen containing only dead sperm yield unrealistically high sperm counts and high cervical mucus acid phosphatase levels. This is apparently due to the translocation of semen from the area of the external os into the mucus sample by the sampling catheter. This problem can be largely prevented by removing a plug of mucus from the external os prior to the catheter sampling procedure.

Topics: Acid Phosphatase; Adult; Cell Count; Cervix Mucus; Coitus; Evaluation Studies as Topic; Female; Humans; Infertility; Insemination, Artificial; Insemination, Artificial, Homologous; Male; Methods; Spermatozoa; Time Factors

The relationship of prostaglandin levels in human seminal fluid to fertility, an accepted one, was tested by dividing 150 men into 3 clinical groups: 1) Group A, 29 fertile men; 2) Group B, 100 men in noninvestigated infertile marriages; and 3) Group C, 21 men in infertile marriages with no abnormal clinical or laboratory findings. Thin layer chromatography was used to measure the various prostaglandin compounds, after alkali treatment. Prostaglandin E content of semen samples below 11 mcg/ml was considered low; the percentage of samples in each group displaying this low content was: 0% in A, 17% in B, and 41% in C. The groups of men also differed statistically significantly from each other, with A and C being most highly significant. In all 3 groups, the concentration of 19-hydroxy and dehydrated prostaglandins remained constant. Because Group C members had been studied extensively for causes of infertility, the finding of low prostaglandin E content in seminal fluid compared with controls (A) and noninvestigated infertile male partners (B) shows that the E compounds are important for reproduction.

Topics: Acid Phosphatase; Female; Fertility; Fructose; Humans; Infertility; Male; Prostaglandins; Semen; Spectrum Analysis; Spermatozoa; Ultraviolet Rays

Topics: Acid Phosphatase; Adult; Endometrium; Female; Humans; In Vitro Techniques; Infertility; Lysosomes; Male; Menstruation; Menstruation Disturbances; Middle Aged

Topics: Acid Phosphatase; Alkaline Phosphatase; Biomedical Research; DNA; Histocytochemistry; Infertility; Infertility, Male; Male; Spermatozoa

Topics: Acid Phosphatase; Esterases; Histocytochemistry; Humans; Infertility; Infertility, Male; L-Lactate Dehydrogenase; Lipids; Male; Testis

Topics: Acid Phosphatase; Biochemical Phenomena; Humans; Infertility; Phosphoric Monoester Hydrolases