acid-phosphatase and Infertility--Female

This experiment investigated the effects of sub-chronic aluminum chloride (AlCl3) exposure on rat ovaries.. Eighty female Wistar (5weeks old) rats, weighed 110-120g, were randomly divided into four treatment groups: control group (CG), low-dose group (LG, 64mg/kg BW AlCl3), mid-dose group (MG, 128mg/kg BW AlCl3) and high-dose group (HG, 256mg/kg BW AlCl3). The AlCl3 was administered in drinking water for 120days. The ovarian ultrastructure was observed. The activities of acid phosphatase (ACP), alkaline phosphatase (ALP), succinate dehydrogenase (SDH), Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase, the contents of Fe, Cu and Zn, and the protein expression of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in the ovary were determined.. The results showed that the structure of the ovary was disrupted, the activities of ALP, ACP, SDH, Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase, the contents of Zn, Fe and the protein expression of FSHR and LHR were lowered, and the content of Cu was increased in AlCl3-treated rats than those in control.. The results indicate that sub-chronic AlCl3 exposure caused the damage of the ovarian structure, the disturbed metabolism of Fe, Zn and Cu and the decreased activities of Na(+)-K(+)-ATPase, Mg(2+)-ATPase and Ca(2+)-ATPase in the ovary, which could result in suppressed energy supply in the ovary. A combination of suppression of energy supply and reduction of expression of FSHR and LHR could inhibit ovulation and corpus luteum development, leading to infertility in female rats.

Topics: Acid Phosphatase; Alkaline Phosphatase; Aluminum Chloride; Aluminum Compounds; Animals; Chlorides; Copper; Female; Infertility, Female; Iron; Ovary; Rats; Rats, Wistar; Receptors, FSH; Receptors, LH; Zinc

To evaluate if the levels of the central regulators of osteoclast activity, osteoprotegerin (OPG) and receptor activator for nuclear factor kappaB-ligand (RANk-L), correlate with bone mineral density (BMD) parameters in premenopausal infertile women.. Cross-sectional study.. Academic infertility practice.. 82 Infertile, but otherwise healthy, premenopausal women< 42 years age presenting for routine care.. BMD assessment; serum samples (cycle days 1 to 3), questionnaire addressing demographics, medical, social, family, and personal histories.. BMD categorized as "Low" (Z -1.0); Biomarkers of bone metabolism (BAP, TRAP, NTX, OPG, RANK-L) and ovarian reserve (FSH, estradiol).. The prevalence of low BMD was 23% (19/82). Significantly lower levels of OPG were observed in association with low BMD compared with normal BMD (1.37 +/- 0.57 versus 2.0 +/- 1.05 pMol/L). Multivariable logistic regression analyses revealed low OPG levels as independent predictors of low BMD after adjusting for parameters known to influence bone density.. We demonstrate an independent relationship between low circulating levels of OPG and low BMD in premenopausal healthy, albeit infertile, women.

Topics: Absorptiometry, Photon; Acid Phosphatase; Adult; Alkaline Phosphatase; Biomarkers; Bone Density; Collagen Type I; Cross-Sectional Studies; Down-Regulation; Estradiol; Female; Follicle Stimulating Hormone, Human; Humans; Infertility, Female; Isoenzymes; Logistic Models; Osteoprotegerin; Peptides; Premenopause; RANK Ligand; Surveys and Questionnaires; Tartrate-Resistant Acid Phosphatase

In the luminal fluid of parous women the acid phosphatase activity was found to be very low and remained virtually unchanged throughout the cycle (10.60 to 18.14 mgp/hr/100 ml). In the infertiles, the activity was generally high with a maximum of 54.37 mgp/hr/100 ml at late follicular phase. It was argued that increased enzyme level in infertiles could have adversely affected the sperm capacitation power as well as the implantation of the fertilized ovum.

Topics: Acid Phosphatase; Adult; Body Fluids; Female; Humans; Infertility, Female; Uterus

Peritoneal fluid from 35 women with endometriosis and from 34 control women was aspirated at laparoscopy and analyzed. No differences in prostanoid levels were found. The peritoneal fluid volume, macrophage concentration, macrophage content, and content of activated macrophages as measured by acid phosphatase staining were all significantly elevated in the endometriosis patients. The macrophages were incubated and the medium was added to the zona-free hamster egg sperm penetration assay. This medium caused a significant decrease in the percentage of ova penetrated in this assay. It is postulated that one of the mechanisms of infertility in women with endometriosis may involve the increased number of activated macrophages and their ability to interfere with sperm-egg interaction.

Topics: 6-Ketoprostaglandin F1 alpha; Acid Phosphatase; Ascitic Fluid; Cell Count; Dinoprost; Endometriosis; Female; Humans; Ibuprofen; Infertility, Female; Laparoscopy; Macrophage Activation; Macrophages; Male; Menstrual Cycle; Prostaglandins E; Prostaglandins F; Radioimmunoassay; Sperm-Ovum Interactions; Thromboxane B2

Cellular components in peritoneal fluid of infertile patients with and without endometriosis were evaluated in 102 patients with Wright's-Giemsa and Papanicolaou stains. The secretory activity of these cells was studied indirectly by assaying acid phosphatase, prostaglandin (PG) F2 alpha and PGE2 and complement components C3c and C4. The results showed that macrophages and lymphocytes were the dominant cells in peritoneal fluid of these patients. These cells were significantly increased in endometriosis patients, as compared with control subjects. In addition, peritoneal fluid acid phosphatase, PGF2 alpha and PGE2, and complement components C3c and C4 were significantly increased in patients with endometriosis. These cellular changes and their activation in peritoneal fluid may explain infertility associated with endometriosis.

Topics: Acid Phosphatase; Adolescent; Adult; Ascitic Fluid; Complement C3; Complement C3c; Complement C4; Dinoprost; Dinoprostone; Endometriosis; Fallopian Tube Patency Tests; Female; Humans; Infertility, Female; Lymphocytes; Macrophages; Peritoneal Neoplasms; Prostaglandins E; Prostaglandins F; Staining and Labeling

Pelvic fluid was collected from 66 women undergoing laparoscopic sterilization or diagnostic laparoscopy for evaluation of infertility. Cells consisting mainly of macrophages were separated, counted, and subjected to histochemical staining for acid phosphatase and myeloperoxidase as markers of cell irritation. Pelvic fluid was analyzed for acid phosphatase, neutral protease, and extractable prostaglandin E2 and F2 alpha. A higher proportion (46% versus 15%) of the macrophages in the group with mild endometriosis exhibited positive staining for acid phosphatase as compared with the fertile group. Pelvic fluid from patients with mild endometriosis had higher acid phosphatase and neutral protease activity than that from fertile patients (p less than 0.05, p less than 0.01). The content of either prostaglandin was not significantly higher in the endometriosis group as compared with the fertile group. The results suggest that mild endometriosis is associated with activation of macrophages and release of active substances into peritoneal fluid that may be responsible for the associated infertility.

Topics: Acid Phosphatase; Dinoprost; Dinoprostone; Endometriosis; Endopeptidases; Female; Humans; Infertility, Female; Macrophages; Neprilysin; Pelvic Neoplasms; Prostaglandins E; Prostaglandins F

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Body Temperature; Cervix Mucus; Curettage; Endometrium; Estrogens; Female; Humans; Infertility, Female; Methods; Pregnanediol; Vaginal Smears

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Endometrium; Female; Glucosephosphate Dehydrogenase; Glucosephosphate Dehydrogenase Deficiency; Histocytochemistry; Infertility, Female; L-Lactate Dehydrogenase; NAD; Oxidoreductases; Pregnancy; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Adult; Citrates; Cystic Fibrosis; Epididymis; Female; Fructose; Humans; Infertility, Female; Infertility, Male; Male; Pregnancy; Semen; Spermatozoa; Vas Deferens