acid-phosphatase and Infectious-Mononucleosis

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Antibody-Dependent Cell Cytotoxicity; DNA Nucleotidylexotransferase; Female; Hematologic Diseases; Histocytochemistry; Humans; Infectious Mononucleosis; Leukemia, Hairy Cell; Leukemia, Lymphoid; Lymphoma; Male; Middle Aged; Phenotype; Skin Neoplasms; T-Lymphocytes

Topics: Acid Phosphatase; DNA; Electron Transport; Humans; Infectious Mononucleosis; Leukemia; Leukemia, Lymphoid; Leukocytes; Lymphocyte Activation; Lymphocytes; Lymphocytosis; RNA

On the basis of their origin and morphologic and cytochemical characteristics, atypical mononuclear cells (AMNC) in the peripheral blood of patients with the syndrome of infectious mononucleosis caused by the Epstein-Barr (EB) virus can be classified into three groups, from I to III. The AMNC groups I are the most numerous (76.36% +/- 2.50%). A larger part of these cells have the characteristics of more or less altered lymphoblasts and the smaller part of large pyroninophilic cells and "reactive immunoblasts", and meant to have developed from T lymphocytes. These are the most numerous in the acute phase of the disease, which correlates with the most significant increase of T lymphocytes values. A very high percentage (84.00% +/- 7.30%) of the AMNC group I contains acid phosphatase isoenzyme 3 and acid non-specific esterase (82.43% +/- 7.76%), a considerable percentage of beta-glucuronidase (58.75% +/- 13.99%), and acid phosphatase isoenzyme 1 (51.20% +/- 10.81%). A small percentage of these cells contains acid phosphatase isoenzyme 5 resistant to the action of L+tartaric acid. In 39.04% +/- 8.72% of the lymphocytes in the peripheral blood of the examined patients and in the two thirds and nearly three fourths of the AMNC group I, the presence of acid phosphatase manifests itself in the form of scattered granules. The positive reaction to non-specific esterase (pH 7.8-8) in 39.72% +/- 8.44% lymphocytes of the peripheral blood of the patients and in 59.26% +/- 10.09% of the AMNC group I manifests itself in the form of scattered granules.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Acid Phosphatase; Adolescent; Adult; Carboxylesterase; Carboxylic Ester Hydrolases; Child; Child, Preschool; Female; Humans; Infectious Mononucleosis; Leukocyte Count; Leukocytes, Mononuclear; Male

Topics: Acid Phosphatase; Diagnosis, Differential; Glycogen; Histocytochemistry; Humans; Infectious Mononucleosis; Lymphadenitis; Lymphocytes; Lymphoma; Naphthol AS D Esterase; Tuberculosis, Lymph Node

Acid phosphatase (AcP) and acid alpha-naphthylacetate esterase (ANAE) were examined in lymphoid cells from 104 patients suffering from various lymphoproliferative disorders and from 8 healthy controls. Enzyme activities were evaluated by means of a scoring system. Scores of AcP and ANAE were higher in normal T cells than non-T cells. In comparison, the activated T cells in infectious mononucleosis showed increased AcP and decreased ANAE reaction. Malignant T lymphoblasts had a distinct granular AcP positivity in contrast to the faint reactivity observed in cALL blasts, whereas ANAE showed negative or weak reaction in both subsets. High scores and distinct staining patterns for both enzymes were found in T CLL and T prolymphocytic leukaemia, clearly different from the weak activities seen in B CLL, B PLL and some B cell lymphomas. The latter, too, could be distinguished by mutual differences in enzyme reactions. High AcP and ANAE scores were also found in hairy cell leukaemia, and the staining patterns together with the tartrate resistance firmly established the diagnosis. Thus, simultaneous determinations of AcP and ANAE can be of great value in the diagnosis of lymphoid malignancies.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; B-Lymphocytes; Carboxylic Ester Hydrolases; Child; Female; Humans; Infectious Mononucleosis; Leukemia; Lymphocytes; Lymphoma; Male; Middle Aged; Naphthol AS D Esterase; Rosette Formation; Staining and Labeling; T-Lymphocytes

Topics: Acid Phosphatase; Adolescent; Adult; Humans; Infectious Mononucleosis; Lymphocytes; Ribonucleoproteins

Topics: Acid Phosphatase; Adolescent; Adult; Child; Child, Preschool; Esterases; Female; Glucuronidase; Humans; Infectious Mononucleosis; Isoenzymes; Lymphocytes; Male

Topics: Acid Phosphatase; Adolescent; Adult; Cells, Cultured; Enzyme Activation; Histocytochemistry; Humans; Infectious Mononucleosis; Lymphocyte Activation; Lymphocytes; Phytohemagglutinins

Atypical lymphocytes from nine young adults with acute infectious mononucleosis (IM) were studied for morphologic, ultrastructural, cytochemical, and membrane surface marker characteristics. There was an absolute increase in T lymphocytes in the patients. Atypical lymphocytes accounted for 83%-96% of the lymphocyte population. These lymphocytes contained cytoplasmic inclusions which ranged in size from 1000 to 6000 A, were usually membrane bound, and consisted of parallel arrays of microtubulelike structures. The inclusions, which have been referred to as parallel tubular arrays (PTA), were found in 15%-75% of the lymphocytes from the IM patients. Ultrastructural cytochemical methods demonstrated acid phosphatase activity within many of the membrane-bound PTA. The function of the PTA is unknown. Since they were observed only in the lymphocytes which appeared to correspond to the atypical lymphocytes on light microscopy, the majority of which typed as T cells, there appears to be an association between PTA and T lymphocytes. It is possible that PTA identify a specific subset of T lymphocytes which is expanded in IM. Alternatively, PTA may be a transient finding in lymphocytes appearing only in certain biologic states of the cell such as during T-lymphocyte activation.

Topics: Acid Phosphatase; Histocytochemistry; Humans; Inclusion Bodies; Infectious Mononucleosis; Lymphocyte Activation; Lymphocytes; Microtubules; T-Lymphocytes

The present paper gives a report on changes of enzyme reactions, of the glycogen content, and the nucleolus picture in lymphocytes which are based on cytochemical investigations of blood smears taken from 110 children with different diseases. In 20 new-born babies the cytochemical responses of lymphocytes after triple immunization with Di-Pe-Te immunization matter were observed. The findings reveal significant changes to be found predominantly in the activity of alpha-naphthylacetate esterase, PAS-reaction and the nucleolus picture of lymphocytes in immunological reactions. No hints for specific immunological functions of lymphocytes could be detected. The changes may refer to B-lymphocytes and to T-lymphocytes.

Topics: Acid Phosphatase; Alkaline Phosphatase; Antibody Formation; Bacterial Infections; Cell Nucleolus; Child; Child, Preschool; Esterases; Histocytochemistry; Humans; Infant; Infant, Newborn; Infectious Mononucleosis; Leukocytes; Lymphocytes; Naphthaleneacetic Acids; Periodic Acid

Topics: Acid Phosphatase; Animals; Animals, Newborn; Cat-Scratch Disease; Glucuronidase; Hexosaminidases; Humans; Infectious Mononucleosis; Leukemia, Lymphoid; Lymphocyte Activation; Lymphocytes; Muramidase; Rats; Waldenstrom Macroglobulinemia

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Child; Child, Preschool; Humans; Infectious Mononucleosis; Leukocytes; Neutrophils; Succinate Dehydrogenase

Topics: Acid Phosphatase; Autoradiography; Basophils; Cells, Cultured; Cytoplasm; Cytoplasmic Granules; DNA; Esterases; Histocytochemistry; Humans; Infectious Mononucleosis; Leukocyte Count; Lymphocyte Activation; Lymphocytes; Nucleic Acids; RNA; Thymidine; Tritium; Uridine

Topics: Acid Phosphatase; Chronic Disease; Clinical Enzyme Tests; Esterases; Hepatitis A; Histocytochemistry; Humans; Infectious Mononucleosis; Leukemia; Leukemia, Lymphoid; Lymphatic Diseases; Lymphatic System; Lymphocytes; Methods; Monocytes; Naphthols; Peroxidases; Reticulocytes; Staining and Labeling

Topics: Acid Phosphatase; Adolescent; Adult; Antibodies, Heterophile; Autoradiography; Blood Cell Count; Blood Cells; Cell Nucleolus; Cell Nucleus; Child; Child, Preschool; Culture Techniques; Cytoplasmic Granules; DNA; Esterases; Histocytochemistry; Humans; Infectious Mononucleosis; Leukocyte Count; Leukocytes; Liver; Lymph Nodes; Spleen; Staining and Labeling; Thymidine; Tritium; Uridine

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Blood Proteins; Culture Techniques; Female; Glucuronidase; Hodgkin Disease; Humans; Infectious Mononucleosis; Lectins; Leukemia, Lymphoid; Lymphocyte Activation; Lymphocytes; Lymphoma, Follicular; Lysosomes; Malate Dehydrogenase; Male; Middle Aged; Plasmacytoma; Sarcoidosis; Waldenstrom Macroglobulinemia

Topics: Acid Phosphatase; Adolescent; Adult; Basophils; Blood Cell Count; Child; Child, Preschool; Cytoplasm; DNA; Eosinophils; Humans; Infant; Infectious Mononucleosis; Lymphocytes; Male; Monocytes; Mononuclear Phagocyte System; Proteins; RNA

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Cell Count; Histocytochemistry; Humans; Infectious Mononucleosis; Lymphocytes; Monocytes; Staining and Labeling