acid-phosphatase and Infant--Newborn--Diseases

Multiple subcutaneous plaques and nodules appeared on the back and the dorsal proximal area of the extremities of a 9-day-old male infant after a complicated prenatal period necessitating cesarean section. The clinical and histological features were diagnostic of subcutaneous fat necrosis of the newborn. Light microscopy revealed adipocyte necrosis, a lymphohistiocytic infiltrate, and needle-shaped clefts within adipocytes and macrophages. Ultrastructurally, there were aggregations of electron-lucent spaces in the form of spindles and needles arranged in parallel within the altered adipocytes; macrophages surrounded these cells or their fragments and invaded the fat lobules. Enzyme histochemical staining, not previously reported in the literature, showed that acid phosphatase, leucine aminopeptidase, and indoxyl and non-specific esterases were present in the areas of fat necrosis.

Topics: Acid Phosphatase; Adipose Tissue; Carboxylesterase; Carboxylic Ester Hydrolases; Histocytochemistry; Humans; Indoles; Infant, Newborn; Infant, Newborn, Diseases; Leucyl Aminopeptidase; Male; Microscopy, Electron; Necrosis; Skin Diseases

Topics: Acid Phosphatase; Adolescent; Child; Child, Preschool; Enterocolitis, Pseudomembranous; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases

Three cases of infantile digital fibromatosis were studied by electron microscopy, enzyme histochemistry, and tissue culture. The tumors were made up equally of myofibroblasts containing electron-dense inclusions which were composed chiefly of microfilaments measuring about 5 to 7 nm. Dense bodies usually observable in the smooth muscle cells were found in the bundles of these microfilaments and in the process of the inclusions, suggesting that these inclusions may represent an abnormal accumulation of contractile protein in the cytoplasm of tumor cells. Two cell lines were established from culture of the tumor cells, and the cultured cells also contained inclusion bodies showing the same morphologic characteristics as those of the original tumor cells. Lysosomal enzymes were abundant in the cultured cells, but they were scant in the cells of the fresh tissue specimens. Cocultivation of the cultured cells with human embryonic lung cells yielded no cytopathic effect.

Topics: Acid Phosphatase; Culture Techniques; Female; Fibroma; Fingers; Histocytochemistry; Humans; Inclusion Bodies; Infant; Infant, Newborn; Infant, Newborn, Diseases; Male; Skin Neoplasms; Toes

Topics: Acid Phosphatase; Bilirubin; Birth Weight; Erythrocytes; Female; Fetus; Genetics, Medical; Gestational Age; Humans; Infant, Newborn; Infant, Newborn, Diseases; Phenotype; Pregnancy

Topics: Acid Phosphatase; Acute Disease; Antigen-Antibody Reactions; Enzyme Activation; Humans; Immunity, Cellular; Infant, Newborn; Infant, Newborn, Diseases; Leukocytes; Lysosomes; Monocytes; Respiratory Tract Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; Glycerolphosphate Dehydrogenase; Head; Humans; Hypothermia, Induced; Infant, Newborn; Infant, Newborn, Diseases; Leukocytes; Respiratory Insufficiency; Succinate Dehydrogenase

Topics: Acetylcholinesterase; Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Brain; Capillaries; Cholinesterases; Esterases; Gestational Age; Gliosis; Histocytochemistry; Humans; Infant, Newborn; Infant, Newborn, Diseases; Myelin Sheath; Nerve Fibers, Myelinated; Neuroglia; Pyrophosphatases; Sulfatases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adrenalectomy; Fibroblasts; Golgi Apparatus; Hamartoma; Histocytochemistry; Humans; Infant, Newborn; Infant, Newborn, Diseases; Kidney Neoplasms; Male; Microscopy; Microscopy, Electron; Nephrectomy

Topics: Acanthosis Nigricans; Acid Phosphatase; Adenosine Triphosphatases; Child, Preschool; Dermatitis, Exfoliative; Erythema; Esterases; Follow-Up Studies; Glucuronidase; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Keratitis; Male; Nucleotidases; Skin

Topics: Acid Phosphatase; Age Factors; Alkaline Phosphatase; Child; Child, Preschool; Down Syndrome; Esterases; Histocytochemistry; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Leukocytes; Trisomy

Topics: Acid Phosphatase; Adolescent; Blood Bactericidal Activity; Child; Child, Preschool; Chronic Disease; Electrophoresis; Foot; Granuloma; Hand; Histocytochemistry; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Infections; Leukocytes; Lung; Lymph Nodes; Lymphadenitis; Macrophages; Male; Muramidase; Oxygen Consumption; Pedigree; Phagocytosis; Radiography; Serratia marcescens; Staphylococcus; Streptococcus pneumoniae; Tetrazolium Salts