acid-phosphatase and Hypotension

Changes in activity of lysosomal enzymes and levels of adenine nucleotides were investigated during hemorrhagic hypotension and following recovery in rat kidneys. The effect of aprotinin pretreatment on these parameters was also tested, and the results were compared to saline-treated controls. Hemorrhagic hypotension led to a release of lysosomal enzymes and to extreme depletion of ATP. The pretreatment with aprotinin inhibits the changes in enzyme activities, but it has no effect on ATP loss during the hypotension. After reinfusion of the shed blood, however, the pretreated animals showed a faster rate of ATP regeneration. The results suggest an interaction between lysosomal enzyme release and ability of mitochondria to resynthetize high-energy compounds during the recovery period.

Topics: Acid Phosphatase; Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Animals; Aprotinin; Cerebroside-Sulfatase; Energy Metabolism; Glucuronidase; Hypotension; Kidney; Lysosomes; Rats; Shock, Hemorrhagic

The activity of optic density of preparations of cat kidneys by studying lactic, succinic-, glycoso-6-phosphoric-beta-oxybutyrate, NAD.H2-, NADP.H2-tetrazolium-reductases, alkaline and acid phosphatases, was investigated in intact animals, those under hexenal anesthesia, under conditions of a 5-hour hypotension caused by administration of a ganglioblocker (arphonade), 24 hours after the termination of artificial hypotension and in profuse blood letting. Analysis of the optic density distribution curves showed that under conditions of acute vascular insufficiency all the nephron parts reacted by different changes of the enzymatic activity. These changes clearly demonstrated a sharp fall of the energy importance of the main oxidation way (Krebs cycle) and of the prevalence of glycolytic way and pentose shunt under conditions of circulatory hypoxia.

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Animals; Cats; Hemorrhage; Histocytochemistry; Hydroxybutyrate Dehydrogenase; Hypotension; Kidney; Male; NADH, NADPH Oxidoreductases; Succinate Dehydrogenase

The effect of degree and duration of hemorrhagic hypotension on the lysosomal enzyme and LAP levels of plasma of the anaesthesied or wakeful dog has been examined. We ascertained that in a state of hypovolaemia a reduction by 10 mm Hg of the blood pressure resulting significantly increased enzyme activity during the same time. A similar change was caused by prolonging the time. In the anaesthesised group there was the enzyme activity after 120 min of haemorrhagic hypotension lower.

Topics: Acid Phosphatase; Anesthesia; Anesthesia, General; Anesthesia, Local; Animals; Blood Pressure; Dogs; Enzymes; Glucuronidase; Hypotension; Leucyl Aminopeptidase; Lysosomes; Shock, Hemorrhagic; Time Factors

In a study of 4-hr hemorrhagic hypotension in dogs, the plasma levels of the lysosomal enzymes, cathepsin (CATH) and acid phosphatase (AP) showed early and progressive increases in activity. The plasma levels of the intestinal fraction of alkaline phosphatase (IAkP) and aspartate aminotransferase (AAT) were increased after 2 hr of hypotension and the liver specific enzyme, ornithine carbamyltransferase (OCT), and creatine phosphokinase (CPK), after 3 hr. All of the enzymes showed large increases after 4 hr of hypotension. The plasma levels of CATH showed the earliest and largest relative increase indicating that with the shock model used, this enzyme was the most sensitive indicator of shock severity. The increase in plasma enzyme levels was probably the result of tissue damage in the splanchnic region but the elevation of plasma CPK, a muscle specific enzyme, indicates some muscle cell damage as well. While the increase in the plasma enzyme activity is probably due, in large part, to cellular damage, it is likely that a decreased enzyme removal rate--resulting from a hypofunctional RES--also contributes to the elevated plasma enzyme levels during hemorrhagic hypotension.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Cathepsins; Creatine Kinase; Dogs; Hypotension; Lactates; Lysosomes; Male; Ornithine Carbamoyltransferase; Shock, Hemorrhagic

Topics: Acid Phosphatase; Anesthesia, Local; Anesthetics, Local; Animals; Dogs; Glucuronidase; Hypotension; Leucyl Aminopeptidase; Lysosomes; Shock, Hemorrhagic; Wakefulness

Topics: Acid Phosphatase; Biopsy; Brain; Endoplasmic Reticulum; Humans; Hypotension; Infant; Lipids; Liver; Male; Microscopy, Electron; Neurons; Organoids; Psychomotor Disorders; Pyrophosphatases; Reflex; Thiamine Pyrophosphate

Topics: Acid Phosphatase; Animals; Blood Coagulation Tests; Blood Pressure; Cardiac Output; Dogs; Enzymes; Glucuronidase; Hematocrit; Hemodynamics; Hypotension; Injections, Intravenous; Kidney; Leukocyte Count; Lysosomes; Nephrectomy; Pulmonary Circulation; Splenectomy; Time Factors

Topics: Acid Phosphatase; Anaphylaxis; Animals; Antibody Formation; Antigen-Antibody Reactions; Arthus Reaction; Blood Cell Count; Blood Platelets; Blood Pressure; Fluorescent Antibody Technique; Glucuronidase; Hemorrhage; Hydrolases; Hypersensitivity; Hypotension; Immunity, Active; Immunity, Maternally-Acquired; Inflammation; Injections, Intravenous; Leukocytes; Leukopenia; Lung; Lysosomes; Methods; Microscopy, Electron; Microscopy, Fluorescence; Peptide Hydrolases; Pulmonary Circulation; Rabbits; Rats; Serum Albumin, Bovine; Swine

Topics: Acid Phosphatase; Acute Kidney Injury; Animals; Bicarbonates; Cellulose; Chlorides; Chromatography, Ion Exchange; Cystadenoma; Dehydration; Electrophoresis; Female; Glycoproteins; Histocytochemistry; Humans; Hypotension; Middle Aged; Mucoproteins; Potassium; Potassium Isotopes; Rectal Neoplasms; Sodium; Sodium Isotopes; Tritium; Water-Electrolyte Balance