acid-phosphatase and Hepatitis

The zonal distribution of enzyme activities was measured by quantitative cytochemistry in cryosections of liver from three normal children and five infants with idiopathic hepatitis of infancy. Optimal conditions for cytochemical reactions were first validated in rat liver and subsequently used in human livers to quantify zonal activities of acid phosphatase (AP), succinate dehydrogenase (SDH), glutamate dehydrogenase (GDH), glucose-6-phosphatase (G6P) and NADPH-dehydrogenase (ND). In normal rat and human livers, activities were greater for SDH and G6P in periportal and for GDH and ND in perivenular hepatocytes, while AP was evenly distributed along the sinusoids. In five infants with idiopathic hepatitis of infancy (IHI), a similar trend of distribution was observed for the two mitochondrial (SDH and GDH) and the two microsomal (G6P and ND) enzymes, although the distribution gradient was less pronounced than, in normal livers. AP showed a mildly greater periportal than perivenular activity. This preliminary study shows that a similar metabolic zonation exists for these enzymes in human livers as is observed in rats.

Topics: Acid Phosphatase; Animals; Biopsy; Female; Glucose-6-Phosphatase; Glutamate Dehydrogenase; Hepatitis; Histocytochemistry; Humans; Infant; Infant, Newborn; Liver; Male; NADPH Dehydrogenase; Rats; Rats, Inbred Strains; Reference Values; Succinate Dehydrogenase

The authors examined the effect in vivo and in vitro of Catergen (cyanidanol-3) on demonstrable lysosomal enzyme activity in the serum and granulocytes in liver diseases. Acid phosphatase, cathepsin-D and beta-glucuronidase were investigated. In the in vitro studies the direct effect of Catergen was observed by enzyme release. In chronic active hepatitis without treatment the lysosomal activity of the serum increases, the lysosomal activity of the granulocytes decreases and in vitro release increases. Under the effects of Catergen treatment the lysosomal activity of the serum decreases in comparison with initial values, approaching the lysosomal enzyme activity observed in healthy persons. Under in vitro conditions, lysosomal enzyme release from the granulocytes decreases in treated individuals and thus a higher lysosomal enzyme activity in the granulocytes is observed. Granulocytes separated from the blood of healthy persons were incubated with Catergen in isotonic and hypotonic media. In an isotonic medium the release did not change significantly. Under the effect of hypotension the release of lysosomal enzyme increased considerably. In vitro incubation with small doses of Catergen significantly inhibited the degree of release. On the basis of these data it may be supposed that Catergen has a stabilizing effect on the lysosomal membrane and is thus beneficial in the treatment of liver injuries (alcoholic, toxic, inflammatory) with lysosomal membrane alterations.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Benzopyrans; Catechin; Cathepsin D; Cathepsins; Cell Membrane Permeability; Female; Free Radicals; Glucuronidase; Granulocytes; Hepatitis; Hepatitis, Chronic; Humans; Lysosomes; Male; Middle Aged

The authors have measured the activity of acid phosphatase, beta-glucuronaidase and cathepsin-D in tthe sera of patients with different liver diseases, and the actvity of beta-glucuronidase in granulocytes and the rate of its release. Using the method for the releaseof beta-glucuronidase from the granulocytes they drew conclusions to thedegree of the membrane-damage occurring in the liver diseases. They provided that in the sera of patients with different liver diseases the increase in the activity of the acid phosphatase and the beta-glucuronidase is different. According to their in vitro studies the decrease in the beta-glucuronidase activity measured in the granulocytes, and the release of enzyme is in connection with the type of liver disease and with the different degree of lysosomal membrane alteration.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Chronic Disease; Female; Glucuronidase; Granulocytes; Hepatitis; Humans; Liver Cirrhosis; Liver Diseases; Lysosomes; Male; Middle Aged

High-performance liquid chromatography was used to assay serum acid and alkaline phosphatase. Samples were incubated with adenosine-5'-monophosphoric acid (AMP) in a buffer of required pH, 5'-nucleotidase was inhibited with Ni2+ ions, and the phosphatase activity was determined by measuring the concentration of the reaction product, adenosine. The analysis time, after the incubation is terminated, is short (7 min), and the assay is quantitative and reproducible. Complete separation of the reaction product from the substrate and the naturally occurring serum constituents and the high sensitivity of the ultraviolet detection system eliminate some of the problems commonly encountered in spectrophotometric assays.

Topics: Acid Phosphatase; Adenosine; Alkaline Phosphatase; Chromatography, High Pressure Liquid; Hepatitis; Humans; Liver Cirrhosis; Nucleotidases; Reference Values

Topics: Acid Phosphatase; Adolescent; Child; Chronic Disease; Citric Acid Cycle; Female; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Hepatitis; Humans; Liver Cirrhosis; Lymphocytes; Male; Prednisolone

Topics: Acid Phosphatase; Adult; Biopsy; Female; Hepatitis; Humans; Hyalin; Inclusion Bodies; Liver; Liver Cirrhosis; Male; Microscopy, Electron; Middle Aged

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aged; Biopsy; Female; Glucuronidase; Hepatitis; Histocytochemistry; Humans; Liver; Liver Diseases; Male; Middle Aged

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Aminopeptidases; Animals; Bile Ducts, Intrahepatic; Cattle; Fluorescent Antibody Technique; Hepatitis; Histocytochemistry; Histological Techniques; Naphthalenes

Topics: Acid Phosphatase; Animals; Carboxypeptidases; Cathepsins; Deoxyribonucleases; Female; Galactosamine; Glucuronidase; Hepatectomy; Hepatitis; Hexosamines; Histocytochemistry; Liver; Liver Regeneration; Lysosomes; Peptide Hydrolases; Rats

Topics: Acid Phosphatase; Adolescent; Child; Child, Preschool; Chronic Disease; Esterases; Fatty Liver; Glycerolphosphate Dehydrogenase; Hepatitis; Histocytochemistry; Humans; Hypertension, Portal; Liver; Liver Cirrhosis; Liver Diseases; Malate Dehydrogenase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Blood Proteins; Chronic Disease; Depression, Chemical; Diet Therapy; Female; Hepatitis; Hepatolenticular Degeneration; Histocytochemistry; Humans; Iron; Lipase; Liver; Liver Cirrhosis; Liver Function Tests; Male; Penicillamine; Potassium; Pyridoxine; Radionuclide Imaging; Stimulation, Chemical; Sulfides; Vitamin B 12

Topics: Acid Phosphatase; Acute Disease; Adult; Alkaline Phosphatase; Female; Hepatitis; Humans; Jaundice; Liver; Microscopy, Electron; Middle Aged

Topics: Acid Phosphatase; Aged; Alkaline Phosphatase; Bilirubin; Biopsy; Blood Cell Count; Blood Protein Disorders; Blood Protein Electrophoresis; Blood Sedimentation; Bone Marrow Examination; Carcinoma, Hepatocellular; Cholesterol; Hepatitis; Humans; Immunoelectrophoresis; Immunoglobulin G; Liver Cirrhosis; Liver Neoplasms; Male; Neoplasm Metastasis; Phosphates; Transaminases

Topics: Acid Phosphatase; Aldehyde-Lyases; Alkaline Phosphatase; Anemia; Animals; Aspartate Aminotransferases; Carbon Tetrachloride; Erythrocyte Count; Erythrocytes, Abnormal; Erythropoiesis; Glucosephosphate Dehydrogenase; Hepatitis; Humans; L-Lactate Dehydrogenase; Oxidoreductases; Phenylhydrazines; Rabbits; Reticulocytes

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Bilirubin; Birth Weight; Female; Hepatitis; Humans; Infant; Infant, Newborn; Liver; Microscopy, Electron; Pregnancy; Pregnancy Complications, Infectious; Rubella; Rubella virus

Topics: Acid Phosphatase; Adenosine Triphosphatases; Amino Acids; Animals; Asparagine; Aspartic Acid; Bilirubin; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Esterases; Glucose-6-Phosphatase; Hepatitis; Histocytochemistry; Humans; Lipid Metabolism; Liver; Liver Function Tests; Nucleic Acids; Oxidoreductases; Rats; Sulfobromophthalein

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Biological Transport; Chronic Disease; Dihydrolipoamide Dehydrogenase; Electron Transport Complex IV; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Glycogen; Hepatitis; Histocytochemistry; Humans; Liver; Liver Cirrhosis; Malate Dehydrogenase; Methods; Protein Biosynthesis; RNA; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Clinical Enzyme Tests; Dihydrolipoamide Dehydrogenase; Electron Transport Complex IV; Glucose-6-Phosphatase; Hepatitis; Hepatitis A; Hepatitis, Animal; Histocytochemistry; Isocitrate Dehydrogenase; Liver; Mice; Murine hepatitis virus; Oxidoreductases; Pathology; Phosphoric Monoester Hydrolases; Research; Succinate Dehydrogenase

1. An investigation has been made of the changes occurring in lysosomal enzyme activities during the early development of experimentally produced liver injury in the rat. Three enzymes have been studied: acid phosphatase, acid ribonuclease and beta-glucuronidase. Four different methods of inducing liver injury have been used: administration of carbon tetrachloride, thioacetamide, dimethylnitrosamine and the fungal toxin sporidesmin. 2. The majority of the data presented concern alterations produced by carbon tetrachloride. Despite the extensive central necrosis and accompanying fat accumulation which this poison produced in the liver, only small changes in the activity and latency of lysosomal enzymes could be detected. In the early (pre-necrotic) period of injury these changes were insignificant. At a late stage of injury, when extensive centrilobular necrosis was present, there were indications of lysosomal rupture. 3. The results obtained with the other three hepatotoxins were similar to those described for carbon tetrachloride in that no evidence of early lysosomal rupture was obtained during the pre-necrotic period. It is concluded that lysosomes probably play no role in the early development of the four types of liver injury studied but, instead, are involved in later scavenging processes.

Topics: Acid Phosphatase; Amides; Animals; Blood; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Glucuronidase; Hepatitis; Liver; Lysosomes; Nitrosamines; Promethazine; Rats; Ribonucleases; Sulfhydryl Compounds

Topics: Acid Phosphatase; Alkaline Phosphatase; Hepatitis; Histocytochemistry; Humans; Liver; Liver Cirrhosis

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Esterases; Hepatitis; Hepatitis A; Histocytochemistry; Liver; Mice; Murine hepatitis virus; Research

Topics: Acid Phosphatase; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Cytoplasm; Electrons; Fatty Liver; Hepatitis; Histocytochemistry; Humans; Liver; Lysosomes; Microscopy; Microscopy, Electron; Rats; Research

Topics: Acid Phosphatase; Alanine Transaminase; Biopsy; Biopsy, Needle; gamma-Globulins; Glucuronidase; Hepatitis; Humans; Liver; Liver Cirrhosis; Lysosomes; Research; Serum Albumin

Topics: Acid Phosphatase; Chemical and Drug Induced Liver Injury; Copper; Electrons; Hepatitis; Histocytochemistry; Liver; Microscopy; Microscopy, Electron; Pigments, Biological; Rats; Research; Toxicology

Topics: Acid Phosphatase; Alanine Transaminase; Aldehyde-Lyases; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Clinical Enzyme Tests; D-Alanine Transaminase; Fructose-Bisphosphate Aldolase; Geriatrics; Hepatitis; Jaundice; L-Lactate Dehydrogenase; Malate Dehydrogenase

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Chemical Analysis; Clinical Enzyme Tests; Communicable Diseases; Diagnosis, Differential; Fructose-Bisphosphate Aldolase; Hemagglutination; Hepatitis; Hepatitis A; Humans; Transaminases

Topics: Acid Phosphatase; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Ethionine; Fluorenes; Hemosiderin; Hepatitis; Injections; Injections, Subcutaneous; Iron; Iron-Dextran Complex; Liver; Proteins; Rats; Research; Toxicology

Topics: 2-Acetylaminofluorene; Acid Phosphatase; Chemical and Drug Induced Liver Injury; Dextrans; Electrons; Fluorenes; Hemosiderin; Hepatitis; Iron; Iron-Dextran Complex; Liver; Microscopy; Microscopy, Electron; Rats; Research; Toxicology

Topics: Acid Phosphatase; Alkaline Phosphatase; Bile; Cell Nucleus; Cholestasis; Cytomegalovirus Infections; Giant Cells; Hepatitis; Humans; Infant; Infant, Newborn; Jaundice; Liver; Liver Diseases; Liver Regeneration; Pathology

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Amides; Bile; Bile Ducts; Bile Ducts, Intrahepatic; Capillaries; Chemical and Drug Induced Liver Injury; Hepatitis; Histocytochemistry; Jaundice; Jaundice, Obstructive; Pathology; Research; Sulfhydryl Compounds; Thiocyanates; Toxicology

Topics: Acid Phosphatase; Alkaline Phosphatase; Carbon Tetrachloride; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Hepatitis; Kidney; Liver; Mice; Pharmacology; Research; Toxicology; Uranium; Uranyl Nitrate

Topics: Acid Phosphatase; Alkaline Phosphatase; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Hepatitis; Kidney; Liver; Liver Cirrhosis; Phosphoric Monoester Hydrolases; Polysaccharides; Rats; Research; Toxicology