acid-phosphatase and Hemosiderosis

Topics: Acid Phosphatase; Centrifugation, Density Gradient; Esterases; Galactosidases; Glucosidases; Glucuronidase; Hemochromatosis; Hemosiderosis; Hexosaminidases; Histocytochemistry; Iron; Lysosomes

The peroxidatic activity of hemoglobin permitted visualization of its uptake by rat hepatocytes by means of the Graham-Karnovsky 3,3'-diaminobenzidine (DAB) procedure. Lysosomes were visualized by their acid phosphatase, beta-glucuronidase, and glucosaminidase activities. When large doses of rat, cow, or human hemoglobin are intravenously injected, or when hemoglobinemia is induced by injection of distilled water, DAB-positive hemoglobin is engulfed by pinocytosis. Pinocytotic vacuoles become digestive vacuoles ("phagolysosomes") by fusion with lysosomes of the dense body type that have moved from their pericanalicular position. By 16-24 hr after even massive amounts of hemoglobin (400 mg/100 g), the protein is barely demonstrable in hepatocytes. At the lowest doses of injected hemoglobin (15 mg/100 g body weight), DAB-positive vacuoles are demonstrable only in the Kupffer cells.

Topics: Acid Phosphatase; Animals; Cattle; Glucosyltransferases; Glucuronidase; Hemoglobins; Hemosiderosis; Humans; Injections, Intravenous; Liver; Liver Diseases; Lysosomes; Microscopy, Electron; Peroxidases; Pinocytosis; Rats; Time Factors

Topics: Acid Phosphatase; Animals; Chronic Disease; Estrus; Female; Hemosiderosis; Histocytochemistry; Kidney; Kidney Tubules; Microscopy, Electron; Pregnancy; Rats; Starvation