acid-phosphatase and Hematologic-Diseases

Topics: Acid Phosphatase; Biomarkers; Clinical Enzyme Tests; Diagnosis, Differential; Hematologic Diseases; Hematologic Tests; Humans; Leukemia, Hairy Cell; Leukocytes; Reference Values; Specimen Handling; Tartrates

The diagnosis of mastocytosis or mast cell disease may be difficult sometimes because of the wide variety of clinical presentation, abnormal morphology of mast cells, and variation in histologic features which may mimic varieties of other diseases. Over the years, several cell type specific cytochemical and immunochemical markers have been used for the identification of hematopoietic cells in order to establish the accurate diagnosis of mastocytosis and their associated hematologic diseases. Cytochemical stain for aminocaproate esterase is the most specific enzyme marker for identification of mast cells on cytologic specimens and the immunohistochemical stain for tryptase and/or c-kit has also been established as a sensitive and specific marker for mast cells in paraffin sections.

Topics: Acid Phosphatase; Adult; Age of Onset; Biomarkers; Carboxylic Ester Hydrolases; CD56 Antigen; Child; Coloring Agents; Cytokines; Cytoplasmic Granules; Disease Progression; Hematologic Diseases; Humans; Isoenzymes; Mast Cells; Mastocytosis; Paraffin Embedding; Platelet Glycoprotein GPIIb-IIIa Complex; Prognosis; Proto-Oncogene Proteins c-kit; Receptors, Cytokine; Sensitivity and Specificity; Serine Endopeptidases; Staining and Labeling; Tartrate-Resistant Acid Phosphatase; Tryptases; Urticaria Pigmentosa

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Antibody-Dependent Cell Cytotoxicity; DNA Nucleotidylexotransferase; Female; Hematologic Diseases; Histocytochemistry; Humans; Infectious Mononucleosis; Leukemia, Hairy Cell; Leukemia, Lymphoid; Lymphoma; Male; Middle Aged; Phenotype; Skin Neoplasms; T-Lymphocytes

Topics: Acid Phosphatase; Blood Platelet Disorders; Bone and Bones; Bone Diseases; Breast Neoplasms; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Female; Hematologic Diseases; Humans; Isoenzymes; Leukemia; Leukocytes; Lipidoses; Male; Primary Myelofibrosis; Prostate; Prostatic Neoplasms; Spleen; Thromboembolism

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Aminopeptidases; Esterases; Glucosephosphate Dehydrogenase; Glucuronidase; Hematologic Diseases; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Peroxidases; Succinate Dehydrogenase

Topics: Acid Phosphatase; Acyltransferases; Adenosine Triphosphatases; Clinical Trials as Topic; Esterases; Glucose-6-Phosphatase; Hematologic Diseases; Histocytochemistry; Humans; Phosphoric Monoester Hydrolases; Thiamine Pyrophosphate

We report on a case of osteoclast-poor osteopetrosis who received a hematopoietic stem cell graft and, despite hematological engraftment, showed little signs of response in the skeletal defect. Clinical and laboratory studies supported the concept that the bone microenvironment remained abnormal, thus reducing the clinical response to transplantation.. Osteopetrosis is a rare genetic disorder characterized by severely reduced bone resorption resulting from a defect in either osteoclast development (osteoclast-poor osteopetrosis) or activation (osteoclast-rich osteopetrosis). Patients with osteoclast-rich osteopetrosis can be rescued by allogenic hematopoietic stem cell transplantation; however, little information exists concerning the success of transplantation as a treatment for osteoclast-poor osteopetrosis. We report on a child with osteoclast-poor osteopetrosis whose diagnosis was delayed, consequently receiving a cord blood transplant from an unrelated donor at the age of 8 years. Engraftment was deemed successful by peripheral blood genotyping, although >3 years after transplantation there was little rescue of the skeletal defect and anemia, and extramedullary hematopoiesis persisted.. Peripheral blood mononuclear cells from the osteopetrosis patient, before and after transplantation, were used to generate osteoclasts in vitro in the presence of macrophage colony-stimulating factor (M-CSF) and RANKL.. Before transplantation few, small mononuclear osteoclasts formed (F-actin ring-positive cells, co-localizing with vitronectin receptor [alphavbeta3 integrin] and TRACP) associated with occasional, small resorption lacunae. Low levels of collagen C-terminal telopeptide (CTx) fragments were released from these cultures as assessed by ELISA (CrossLaps; patient, 12.85 nM; control, 448.6 nM). In contrast, osteoclasts formed in cultures after transplantation formed to a similar degree to control cultures from healthy individuals: large numbers of osteoclasts containing numerous nuclei were present, and approximately 50% of the surface of bone slices was resorbed, associated with intermediate levels of collagen fragment release (116.48 nM). The culture data reflect the histopathology and radiological findings and also support previous studies showing that neither M-CSF nor RANKL rescues osteoclast-poor osteopetrosis.. This is the first case reported in which a successful hematopoietic engraftment failed to correct an osteopetrotic skeletal defect, and this finding may be credited to the age at which the child was transplanted.

Topics: Acid Phosphatase; Biopsy; Carrier Proteins; Cartilage; CD11c Antigen; CD18 Antigens; Cell Differentiation; Child; Collagen; Collagen Type I; Cord Blood Stem Cell Transplantation; DNA Mutational Analysis; Female; Femur; Glycoproteins; Hematologic Diseases; Humans; Humerus; Integrin alphaVbeta3; Isoenzymes; Leukocytes, Mononuclear; Macrophage Colony-Stimulating Factor; Membrane Glycoproteins; Osteoclasts; Osteopetrosis; Osteoprotegerin; Peptides; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Receptors, Cytoplasmic and Nuclear; Receptors, Tumor Necrosis Factor; Tartrate-Resistant Acid Phosphatase; Transplantation, Homologous; Treatment Outcome

The alterations of haematological parameters in albino rats were studied after oral administration of an aqueous extract of silken styles of corn (Zea maize Linn.) at 50, 100 and 150 mg kg-1 daily for 21 days. The following haematological values were significantly reduced on the 7th and 21st day following extract administration: haemoglobin (Hb), red blood corpuscles (RBC), clotting time (CT), mean corpuscular volume (MCV), haematocrit (Ht), serum glucose, blood urea nitrogen (BUN), cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), calcium, total protein, total albumin and total acid phosphatase; and white blood corpuscles (WBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), alkaline phosphatase and creatinine increased. The remaining parameters were not significantly affected, except body weight parameters at the two highest doses. The results emphasize that the biochemical changes caused through aqueous extract of silken styles of corn (Zea maize Linn.) are not significantly toxic at low and medium doses (50 and 100 mg kg-1).

Topics: Acid Phosphatase; Alanine Transaminase; Animals; Aspartate Aminotransferases; Blood Coagulation; Blood Glucose; Blood Proteins; Blood Sedimentation; Blood Urea Nitrogen; Blood Volume; Body Weight; Calcium; Chemical and Drug Induced Liver Injury; Cholesterol; Dose-Response Relationship, Drug; Hematocrit; Hematologic Diseases; Hemoglobins; Leukocytes; Male; Plant Extracts; Rats; Serum Albumin; Zea mays

CB3717 is a quinazoline antifolate whose cytotoxic activity is mediated by inhibition of thymidylate synthase (TS). A phase I clinical trial commenced in September 1981 and 99 patients have received 296 treatments. Doses were dissolved in 0.15 mol/L NaHCO3 (pH 9.0) at a concentration of 4 mg/mL infused over one hour or in a total volume of 1 L infused over 12 hours. Doses were repeated every 3 weeks. The starting dose of 140 mg/m2 was escalated to 600 mg/m2. Renal toxicity, detected by a decrease in the 51Cr EDTA clearance, was dose-related and occurred in seven of ten patients receiving greater than 450 mg/m2. Reversible hepatic toxicity often associated with malaise occurred in 223 of 288 assessable courses (77%). Fifty-nine courses (20%) were associated with increases in alanine transaminase (ALT) levels to greater than 2.5 times the upper limit of the normal laboratory range. Increases in alkaline phosphatase levels also occurred, but were less marked. The severity and prevalence of these elevations were unaffected by the duration of the infusion. A self-limiting rash appeared in 12 patients and a radiation recall reaction was seen in two. Leukopenia developed in 17 patients (WBC less than 3 X 10(9)/L), and thrombocytopenia occurred in six patients (platelets less than 100 X 10(9)/L). The mean leucocyte nadir occurred on day 10 and was followed by recovery at 11 to 19 days. Neither the incidence nor the severity of any of these latter toxicities was dose related. The maximum tolerated dose was in the region of 600 mg/m2 with renal toxicity being dose limiting, although the inter-patient variation did not allow a precise definition. Seventy-six patients were evaluable for response. Responses occurred at doses greater than or equal to 200 mg/m2 and were ovary, one complete response (CR), one partial response (PR), seven minor responses (MR) in 30 cases; breast, two PRs and one MR in eight cases; adenocarcinoma of the lung, one MR in 5 cases; mesothelioma, one PR in five cases; and colon, two MRs in four cases. CB3717 has activity in heavily pretreated patients. The recommended phase II dose for good-risk patients is 400 mg/m2 using the one-hour infusion schedule of administration.

Topics: Acetylglucosaminidase; Acid Phosphatase; Alanine Transaminase; Antineoplastic Agents; Chemical and Drug Induced Liver Injury; Dose-Response Relationship, Drug; Drug Administration Schedule; Drug Evaluation; Folic Acid; Folic Acid Antagonists; Glomerular Filtration Rate; Hematologic Diseases; Hyperbilirubinemia; Kidney Diseases; Leucyl Aminopeptidase; Neoplasms; Quinazolines; Skin Diseases; Thymidylate Synthase

Topics: Acid Phosphatase; Adenocarcinoma; Aged; Alkaline Phosphatase; Bone Marrow; False Negative Reactions; False Positive Reactions; Female; Hematologic Diseases; Humans; Male; Middle Aged; Prostatic Neoplasms

Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Blood Proteins; Esterases; Hematologic Diseases; Hodgkin Disease; Humans; Megakaryocytes; Peroxidases; Polycythemia Vera; Polysaccharides; Thrombocytopenia

Topics: Acid Phosphatase; Adult; Antibiotics, Antineoplastic; Asparaginase; Blood Transfusion; Bone Marrow Examination; Daunorubicin; Drug Combinations; Esterases; Glucuronidase; Hematologic Diseases; Humans; Leukemia; Leukemia, Erythroblastic, Acute; Male; Megakaryocytes; Methotrexate; Peroxidases; Prednisolone; Spleen; Staining and Labeling; Thrombocytopenia; Vincristine

Topics: Acid Phosphatase; Adolescent; Bone Marrow; Bone Marrow Cells; Child; Child, Preschool; Hematologic Diseases; Humans; Leukemia; Lymphoma

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Bone Neoplasms; Bone Resorption; Breast Neoplasms; Female; Hematologic Diseases; Humans; Injections, Intravenous; Male; Middle Aged; Movement; Multiple Myeloma; Neoplasm Metastasis; Pain, Intractable; Radionuclide Imaging; Remission, Spontaneous; Strontium Radioisotopes; Urinary Bladder Neoplasms; Uterine Neoplasms

Topics: Acid Phosphatase; Alanine Transaminase; Amino Acids; Animals; Aspartate Aminotransferases; Catalase; Erythrocytes; Fructose-Bisphosphate Aldolase; Hematologic Diseases; Rabbits; Reticulocytes

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Platelets; Eosinophils; Hematologic Diseases; Histocytochemistry; Humans; Leukocytes; Lymphocytes; Microscopy, Electron; Neutrophils

Topics: Acid Phosphatase; Electrophoresis, Disc; Erythrocytes; Hematologic Diseases; Histocytochemistry; Histological Techniques; Humans; Isoenzymes; Leukocytes

Topics: Acid Phosphatase; Alkaline Phosphatase; Cell Nucleus; Cytogenetics; Eosinophils; Hematologic Diseases; Histocytochemistry; Humans; Metabolism, Inborn Errors; Microscopy, Electron; Mutation; Peroxidases; Phospholipids; Staining and Labeling

Topics: Acid Phosphatase; Adolescent; Adult; Albinism; Animals; Child; Culture Techniques; Cytoplasmic Granules; Female; gamma-Globulins; Glycolipids; Guinea Pigs; Hematologic Diseases; Heterozygote; Homozygote; Humans; Immunodiffusion; Immunoelectrophoresis; Interferons; Leukocytes; Lymphocytes; Lysosomes; Male; Mice; Peroxidases; Phagocytosis; Prednisone; Rabbits; Suspensions; Vincristine

Topics: Acid Phosphatase; Alkaline Phosphatase; Down Syndrome; Electrophoresis; Gels; Hematologic Diseases; Humans; Isoenzymes; Leukocytes

Topics: Acid Phosphatase; Alkaline Phosphatase; Esterases; Hematologic Diseases; Histocytochemistry; Humans; Hydrolases; Methods

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Platelets; Child; Hematologic Diseases; Histocytochemistry; Humans; Megakaryocytes

Topics: Acid Phosphatase; Acute Disease; Age Factors; Alkaline Phosphatase; Animals; Avitaminosis; Bone Neoplasms; Carcinoma; Chronic Disease; Diabetes Mellitus; Esophageal Neoplasms; Estrus; Female; Hematologic Diseases; Hemoglobinuria, Paroxysmal; Histocytochemistry; Hodgkin Disease; Humans; Infections; Leukemia; Leukocytes; Liver Diseases; Lung Neoplasms; Male; Myocardial Infarction; Neutrophils; Pregnancy; Prostatic Neoplasms; Radiation Injuries; Stress, Physiological

Topics: Acid Phosphatase; Animal Diseases; Animals; Carnivora; Cathepsins; Cattle; Cattle Diseases; Escherichia coli; Glucuronidase; Hematologic Diseases; Leukocytes; Lymphocytes; Lysosomes; Microscopy, Phase-Contrast; Muramidase; Neutrophils; Salmonella typhimurium; Streptococcus pyogenes

Topics: Acid Phosphatase; Animals; Blood Cells; Bone Marrow; Bone Marrow Cells; Hematologic Diseases; Histocytochemistry; Humans; Rats

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia; Antineoplastic Agents; Child; Child, Preschool; Hematologic Diseases; Humans; Infant; Leukemia; Purpura

Topics: Acid Phosphatase; Child; Child, Preschool; Cytoplasmic Granules; Female; Hematologic Diseases; Histocytochemistry; Humans; Leukocytes; Lymphocytes; Lysosomes; Male; Microscopy, Electron

Topics: Acid Phosphatase; Female; Hematologic Diseases; Histocytochemistry; Humans; Leukocytes; Male

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Bone Neoplasms; Child; Cytoplasm; Cytoplasmic Granules; Erythrocytes; Female; Hematologic Diseases; Humans; Iron; Male; Middle Aged; Reticulocytes; Splenectomy

Topics: Acid Phosphatase; Alkaline Phosphatase; Communicable Diseases; Diabetes Mellitus; Gastrointestinal Diseases; Gastrointestinal Neoplasms; Heart Diseases; Hematologic Diseases; Humans; Kidney Diseases; Leukocytes; Liver Diseases; Lung Neoplasms; Mental Disorders; Neurologic Manifestations; Thyroid Diseases

Topics: Acid Phosphatase; Hematologic Diseases; Leukemia; Leukocyte Disorders; Leukocytes; Leukocytosis; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Blood; Disease; Hematologic Diseases; Hematopoietic System; Phosphoric Monoester Hydrolases