acid-phosphatase and Heart-Diseases

Topics: Acid Phosphatase; Adrenal Cortex Hormones; Animals; Cardiomegaly; Cathepsins; Centrifugation, Density Gradient; Coronary Disease; Fasting; Heart Diseases; Heart Failure; Hormones; Humans; Hyperthyroidism; In Vitro Techniques; Liver; Lysosomes; Myocardial Infarction; Myocardium; Thyroxine

The effect of acute and repeated action of cobalt, lead, antimony on the metabolism of the heart muscle is shown. High doses of the metals of approximately 1/5 LD50 were studied in the acute experiment and doses one fifth these in the repeated experiment. The data obtained indicated that due to the effect of all the poisons studied changes developed early in catecholamine metabolism - the myocardial noradrenaline content decreased and the blood and urine monamine levels rose. Increase in the content of pyruvic acid, intensification of glycogenolysis processes (increase in phosphorylase a activity and decrease in the glycogen level), and diminished activity of lactate dehydrogenase in the myocardium attested to changes in its metabolism. High doses of the metals caused an increase in the free activity of the lysosome enzymes, cathepsins and of acid phosphatase, which indicated that the permeability of the membranes of these organelles was disturbed. The mechanism of the cardiotoxic effect of the metals is discussed.

Topics: Acid Phosphatase; Animals; Antimony; Catecholamines; Cathepsins; Cobalt; Heart Diseases; L-Lactate Dehydrogenase; Lead Poisoning; Lysosomes; Male; Myocardium; Phosphorylases; Rats

The present study was undertaken to determine the involvement of cardiac lyososomes in injury to the myocardium after cardiopulmonary bypass. Twenty conditioned mongrel dogs, weighing 15 to 18 kilograms, were fasted overnight, anesthetized with sodium pentobarbital (30 mg. per kilogram), intubated, and maintained on positive-pressure ventilation. The femoral artery and femoral vein were cannulated for pressure measurements. After median sternotomy, intravenous heparin was administered (3 mg. per kilogram) before the aorta and the superior and inferior venae cavae were cannulated for bypass. Bypass was instituted with a Travenol modular pump and a Bentley pediatric bubble oxygenator and heat exchanger. The ultrastructural effects on the myocardium and the acid phosphatase activity in the left ventricle were compared in dogs exposed to bypass for 1 hour with varying types of myocardial support: perfusion of the coronary arteries, normothermic ischemic arrest, or selective cardiac hypothermia. The morphology of control hearts and hearts fixed after 1 hour of coronary perfusion were similar. The distribution and structure of subcellular lysosomes were the same and showed identical patterns of acid phosphatase activity. Normothermic ischemic arrest was associated with a loss of glycogen stores, disrupted sarcoplasmic reticulum and T tubules, vacuolization and decrease in matrix density of mitochondria, and separation of the intercalated discs. Lysosomal activity was absent except for occasional residual bodies in the nuclear pole zone of the myocardial cells. Selective cardiac hypothermia produced results superior to those from normothermic ischemic arrest. Although these hearts showed proliferation of the lysosomal compartment, the organelles responsible for excitation-contraction coupling were spared.

Topics: Acid Phosphatase; Animals; Cardiopulmonary Bypass; Coronary Disease; Dogs; Extracorporeal Circulation; Glycogen; Heart Arrest; Heart Diseases; Lysosomes; Mitochondria, Muscle; Myocardium; Sarcoplasmic Reticulum; Time Factors

The plastic metabolism of the myocardium in rabbits was studied after varying periods of allergic damage induced by repeated injections of normal horse serum. An accelerated decompostion of the myofibril proteins was demomstrated to occur during the acute phase. During the reparation phase, an activation of the protein synthesis in the myocardium occurred. The newly synthesized myofibril proteins had a longer turnover in the tissues, than under normal conditions. The turnover time of the mitochondrial proteins did not differ from the normal one.

Topics: Acid Phosphatase; Animals; Heart Diseases; Hydrolysis; Hypersensitivity; Muscle Proteins; Myocardium; Myofibrils; Rabbits

Topics: Acid Phosphatase; Administration, Oral; Antineoplastic Agents; Carbamates; Carcinoembryonic Antigen; Estranes; Heart Diseases; Heart Failure; Humans; Male; Nausea; Neoplasm Metastasis; Nitrogen Mustard Compounds; Organophosphorus Compounds; Prostate; Prostatic Neoplasms; Vomiting

Topics: Acid Phosphatase; Age Factors; Aminopeptidases; Brain; Dipeptides; Duodenum; Female; Heart Diseases; Humans; Isoenzymes; Kidney; Liver; Lung; Male; Myocardium; Naphthalenes; Organ Specificity; Pancreas; Pulmonary Embolism; Sex Factors; Skin; Spleen

Topics: Acid Phosphatase; Adult; Amniocentesis; Cardiomegaly; Female; Fetal Diseases; Genes, Recessive; Glucose; Glucosidases; Glycogen Storage Disease; Heart Defects, Congenital; Heart Diseases; Heterozygote; Humans; Infant, Newborn; Lectins; Lymphocytes; Male; Myocardium; Pregnancy; Prenatal Diagnosis; Syndrome; Time Factors

Topics: Acid Phosphatase; Axons; Brain; Brain Chemistry; Cell Nucleus; Cytoplasm; Glucosidases; Glucosyltransferases; Glycogen; Glycogen Storage Disease; Heart Diseases; Humans; Infant; Lysosomes; Male; Microscopy; Microscopy, Electron; Mitochondria; Myelin Sheath; Nervous System; Nervous System Diseases; Neuroglia; Neurons; Peripheral Nerves; Schwann Cells; Spinal Cord

Topics: Acid Phosphatase; Adaptation, Physiological; Animals; Aortic Coarctation; Deoxyribonucleases; Disease Models, Animal; Heart; Heart Diseases; Hypoxia; Isoproterenol; Lysosomes; Male; Methods; Myocardium; Rats; Ribonucleases

Topics: Acid Phosphatase; Adaptation, Physiological; Altitude; Animals; Aortic Coarctation; Deoxyribonucleases; Heart Diseases; Hypoxia; Isoproterenol; Lysosomes; Male; Myocardium; Rats; Ribonucleases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Azo Compounds; Food Additives; Glucosyltransferases; Heart; Heart Diseases; Histocytochemistry; Lysosomes; Microscopy, Electron; Muscles; Muscular Diseases; Myocardium; Myofibrils; Pigments, Biological; Rats

Topics: Abdominal Injuries; Acid Phosphatase; Adenosine Triphosphatases; Adult; Alkaline Phosphatase; Animals; Brain; Electron Transport Complex IV; Glucose; Glucose-6-Phosphatase; Glucosephosphate Dehydrogenase; Heart Diseases; Hemorrhage; Histocytochemistry; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Malate Dehydrogenase; Oxidation-Reduction; Phosphoric Monoester Hydrolases; Rats; Succinate Dehydrogenase

Topics: Acid Phosphatase; Bone Marrow Diseases; Cardiovascular Diseases; Clinical Enzyme Tests; Heart Diseases; Histocytochemistry; Humans; Leukocytes; Neoplasm Metastasis; Neoplasms; Thyroid Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; Communicable Diseases; Diabetes Mellitus; Gastrointestinal Diseases; Gastrointestinal Neoplasms; Heart Diseases; Hematologic Diseases; Humans; Kidney Diseases; Leukocytes; Liver Diseases; Lung Neoplasms; Mental Disorders; Neurologic Manifestations; Thyroid Diseases

Topics: Acid Phosphatase; Administration, Intravenous; Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Blood Chemical Analysis; D-Alanine Transaminase; Electron Transport Complex II; Heart Diseases; Liver Diseases; Necrosis; Papain; Pathology; Rabbits; Research; Succinate Dehydrogenase