acid-phosphatase and Glycosuria

Curcumin is a potent inhibitor of the transcription factor activator protein-1 which plays an essential role in osteoclastogenesis. However, the effects of curcumin on bone metabolism have not been clarified in vivo. We reported herein the inhibitory effects of curcumin on the stimulated osteoclastic activity in insulin-dependent diabetes mellitus using rats with streptozotocin-induced diabetes. A dietary supplement of curcumin reversed the increase in levels of activity and mRNA of tartrate-resistant acid phosphatase (TRAP) and cathepsin K to control values. A histochemical analysis showed that the increase in TRAP-positive cells in the distal femur of the diabetic rats was reduced to the control level by the supplement. These results suggested that curcumin reduced diabetes-stimulated bone resorptive activity and the number of osteoclasts. When bone marrow cells were cultured with macrophage colony stimulating factor and receptor activator NF-kappaB ligand (RANKL), the increased activity to form TRAP-positive multinucleated cells and the increased levels of mRNA and protein of c-fos and c-jun in the cultured cells from diabetic rats decreased to control levels in the curcumin-supplemented rats. Similarly, the increased expression of c-fos and c-jun in the distal femur of the diabetic rats was significantly reduced by the supplement. These results suggested that curcumin suppressed the increased bone resorptive activity through the prevention of osteoclastogenesis associated with inhibition of the expression of c-fos and c-jun in the diabetic rats.

Topics: Acid Phosphatase; Amino Acids; Animals; Blood Glucose; Body Weight; Bone Marrow Cells; Bone Resorption; Calcium; Cathepsin K; Cell Differentiation; Curcumin; Diabetes Mellitus, Experimental; Dietary Supplements; Eating; Female; Femur; Glycosuria; Hydroxyproline; Isoenzymes; Osteocalcin; Osteoclasts; Rats; RNA, Messenger; Staining and Labeling; Stem Cells; Streptozocin; Tartrate-Resistant Acid Phosphatase

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Amino Acids; Animals; Aspartate Aminotransferases; Dose-Response Relationship, Drug; Glycosuria; Hydrogen-Ion Concentration; Inulin; Kidney Diseases; Kidney Function Tests; L-Lactate Dehydrogenase; Lethal Dose 50; Male; Proteinuria; Rabbits; Time Factors; Uranium

Topics: Acid Phosphatase; Animals; Citrates; Citric Acid Cycle; Endoplasmic Reticulum; Female; Fluorine; Glycosuria; Hematuria; Injections, Intraperitoneal; Ketone Bodies; Kidney; Kidney Tubules; Kidney Tubules, Proximal; Male; Microscopy, Electron; Mitochondria; Proteinuria; Rats

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adrenal Glands; Animals; Blood Glucose; Body Weight; Diabetes Mellitus; Diabetes Mellitus, Experimental; Female; Glucosephosphate Dehydrogenase; Glycosuria; Islets of Langerhans; Kidney; L-Lactate Dehydrogenase; Langerhans Cells; Liver; Male; Microscopy; Muscles; Organ Size; Prediabetic State; Rats; Succinate Dehydrogenase; Thyroid Gland

Topics: Acid Phosphatase; Animals; Blood Glucose; Body Weight; Diabetes Mellitus; Female; Glucose-6-Phosphatase; Glucosephosphate Dehydrogenase; Glucosyltransferases; Glycogen; Glycosuria; Guinea Pigs; Histocytochemistry; Hydrocortisone; Islets of Langerhans; L-Lactate Dehydrogenase; Male; Time Factors