acid-phosphatase and Genital-Diseases--Male

Topics: Acid Phosphatase; Animals; Autoimmune Diseases; Bacterial Infections; Ejaculation; Epididymis; Genital Diseases, Male; Genitalia, Male; Humans; Infertility, Male; Male; Methods; Prostate; Radiography; Semen; Seminal Vesicles; Species Specificity; Urinary Bladder; Vas Deferens

The concentrations of three secretory proteins of the human prostate, including prostatic secretory protein of 94 amino acid residues (PSP94), prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), were measured by enzyme-linked immunosorbent assay (ELISA) in semen from a collective of patients suffering from various inflammatory diseases of the genital tract. In addition, levels of the conventional markers citrate, glucosidase and fructose were determined. As compared with semen from men exhibiting no inflammatory condition, only levels of glucosidase in cases of epididymitis and concentrations of PSP94 in the collective suffering from prostatitis showed significant reductions. The changes in the secretion of PSA, PAP, fructose and citrate in the semen of patients with inflammation of genital tract tissue were not significant at the 95% range of confidence.

Topics: Acid Phosphatase; Antigens, Neoplasm; Biomarkers; Carrier Proteins; Citrates; Citric Acid; Enzyme-Linked Immunosorbent Assay; Fructose; Genital Diseases, Male; Glucosidases; Humans; Male; Prostate; Prostate-Specific Antigen; Prostatic Diseases; Prostatic Secretory Proteins; Semen

The heterogeneity of gamma-glutamyltranspeptidase (GGT) in seminal plasma has been studied using Con A-chromatography. This parameter was then related to the fructose concentration, the acid phosphatase activity, ejaculate volume, sperm density and the number of bacteria per ml. Multivariate regression analysis and stepwise elimination of the least fitting factors, revealed that Con A-binding correlated with the number of bacteria per ml of semen and the acid phosphatase activity with 49% of the variance of GGT-binding being explained by these parameters. This result suggests that glycosylation of seminal GGT is altered by accessory gland infection. Neuraminidase digestion suggests that the pattern of Con A-binding of seminal GGT depends only partly on its sialic acid content.

Topics: Acid Phosphatase; Carbohydrate Metabolism; Chromatography, Affinity; Concanavalin A; gamma-Glutamyltransferase; Genital Diseases, Male; Humans; Infections; Male; Neuraminidase; Prostatitis; Semen

Sperm examination, quantitative sperm culture, citric acid, acid phosphatase, and fructose were assayed in three groups of men: fertile controls without significant bacteriospermia (group I), infertile men with significant bacteriospermia; idiopathic infertile men (group II), and infertile men with varicocele (group III). Level of significance of bacteriospermia was greater than or equal to 10(4) germs/ml of ejaculate. In group II, motility and typical morphology percentages were lower, independently of the degree and the nature of bacteriospermia. Incidence of pathogenic bacteria was higher than in group III and linked to the degree of bacteriospermia. Fructose was unaltered in the two groups of infected men. No modification of prostatic markers was observed in any groups, except in group II, where they decreased when bacteriospermia was lower than 10(5) germs/ml and when biological pattern of semen evoked chronic prostatitis. Thus, the presence of germs in ejaculate alters the motility and the typical morphology percentages but does not result in any obvious modifications of biochemical markers of prostate and seminal vesicles. For idiopathic infertile men, it is suggested that the quantitative criterion of pathogenic bacteriospermia is a germ count greater than or equal to 10(5)/ml.

Topics: Acid Phosphatase; Adult; Citrates; Citric Acid; Fructose; Genital Diseases, Male; Humans; Infertility, Male; Male; Middle Aged; Prostate; Semen; Seminal Vesicles; Sperm Count; Spermatozoa; Varicocele

Topics: Acid Phosphatase; Adult; Aged; Clinical Enzyme Tests; Female; Genital Diseases, Male; Humans; Male; Middle Aged; Prostatic Hyperplasia; Prostatic Neoplasms; Radioimmunoassay; Urologic Diseases

Pathological seminal liquids were studied by using cellulose acetate electrophoresis as well as biochemical doses of proteins, acid phosphatases, reducing sugars, and zinc. It was also possible to show the frequent total absences of vesicular secretion, less frequent than prostatic secretion and less than the two at the same time. It was also shown that reductions were more frequent in prostatic secretion than in vesicular secretion. This method may also be a valuable assistance in the diagnosis of certain urogenital infections. The dissociation between prostatic secretions of acid phosphatases and zinc may lead to the diagnosis of the infection in the posterior or lateral lobes of the prostate.

Topics: Acid Phosphatase; Carbohydrate Metabolism; Electrophoresis, Cellulose Acetate; Genital Diseases, Male; Humans; Infertility, Male; Male; Prostate; Proteins; Semen; Seminal Vesicles; Urologic Diseases; Zinc

The diagnosis of male adnexitis is difficult and the influence of this condition on fertility is still a matter of debate. With the intention to define diagnostic criteria a comprehensive study of biochemical and morphological features of semen, plus culture for microorganisms, was performed in patients who were assessed for infertility during a four year period. The following parameters were considered of diagnostic value: a) history of urogenital infection and/or abnormal rectal palpation. b) significant alterations in the expressed prostatic fluid and/or urinary sediment after prostatic massage. c) 1. Uniform growth of more than 10(3) pathogenic bacteria, or more than 10(4) non-pathogenic bacteria per ml, in culture of diluted seminal plasma. c) 2. Presence of more than 10(6) (peroxidase positive) leucocytes per ml of ejaculate. c) 3. Signs of disturbed secretory function of the prostate or seminal vesicles. The diagnosis of infection is accepted if either of the following combinations if found: a + b, a + c (1 or 2 or 3), b + c (1 or 2 or 3), c1 + c2, c1 + c3, c2 + c3.

Topics: Acid Phosphatase; Bacterial Infections; Fructose; Genital Diseases, Male; Humans; Infertility, Male; Leukocytes; Male; Prostate; Prostatitis; Semen; Seminal Vesicles

Topics: Acid Phosphatase; Adolescent; Adult; Aged; alpha-Fetoproteins; Animals; Carcinoembryonic Antigen; Child; Chorionic Gonadotropin; Clinical Enzyme Tests; Dogs; Genital Diseases, Male; Humans; Immunoenzyme Techniques; Infant; Infertility, Male; Male; Middle Aged; Prostatic Neoplasms; Testicular Neoplasms; Testis; Urinary Bladder; Urinary Bladder Neoplasms; Urologic Diseases

Topics: Acid Phosphatase; Biopsy; Clinical Enzyme Tests; Cystoscopy; Diagnosis, Differential; Genital Diseases, Male; Humans; Male; Prostatic Neoplasms; Urinary Bladder Neoplasms; Urologic Diseases

Topics: Acid Phosphatase; Autoimmune Diseases; Epididymis; Fructose; Genital Diseases, Male; Humans; Male; Orchitis; Sperm Agglutination; Spermatozoa

Topics: Acid Phosphatase; Adolescent; Adult; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Blood Cells; Calcium; Genital Diseases, Male; Humans; Infertility, Male; L-Lactate Dehydrogenase; Malate Dehydrogenase; Male; Middle Aged; Potassium; Semen; Sodium; Spermatozoa

Topics: Acid Phosphatase; Adolescent; Adult; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Genital Diseases, Male; Humans; Male; Middle Aged; Potassium; Sodium; Spermatozoa; Time Factors