acid-phosphatase and Gas-Gangrene

acid-phosphatase has been researched along with Gas-Gangrene* in 2 studies

Other Studies

2 other study(ies) available for acid-phosphatase and Gas-Gangrene

Article	Year
Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water. Journal of microbiological methods, 2011, Volume: 87, Issue:2 Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accomplished before. For this purpose, a species specific PCR method was selected based on the results received for type strains and genotypically characterised environmental strains. For the comparative investigation type strains as well as presumptive C. perfringens isolates from water and faeces samples were used. The acid phosphatase test revealed higher percentage (92%) of correctly identified environmental strains (n=127) than the ISO_LGMN procedure (83%) and proved to be a sensitive and reliable confirmation method. Topics: Acid Phosphatase; Bacterial Proteins; Clostridium perfringens; Enzyme Assays; Feces; Gas Gangrene; Humans; Polymerase Chain Reaction; Water Microbiology	2011
Histochemical and electron microscopical studies of skeletal muscle affected by gas gangrene. Folia histochemica et cytochemica, 1975, Volume: 13, Issue:3-4 Ultrastructural and histochemical changes of skeletal muscle were studied in three patients affected with gas gangrene. There was complete lack of the phosphorylase, succinate dehydrogenase and adenosine triphosphatase activities in the affected muscles of all the patients. In unaffected muscles these enzymes showed weaker activities than in norm. The lactate dehydrogenase activity, especially the heart type isozyme (LDH-1 or H4) proved less sensitive to the effect of clostridial toxin. A general increase in the acid phosphatase activity was found both in affected and in unaffected muscles. On electron microscopic examination damage to sarcolemmal membrane and disintegration of myofilaments was seen. The mitochondria were swollen and their cristae distorted and fragmented. Topics: Acid Phosphatase; Adult; Female; Gas Gangrene; Histocytochemistry; Humans; Isoenzymes; L-Lactate Dehydrogenase; Male; Microscopy, Electron; Middle Aged; Mitochondria, Muscle; Muscles	1975

Article

Year

Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water.

Journal of microbiological methods, 2011, Volume: 87, Issue:2

Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accomplished before. For this purpose, a species specific PCR method was selected based on the results received for type strains and genotypically characterised environmental strains. For the comparative investigation type strains as well as presumptive C. perfringens isolates from water and faeces samples were used. The acid phosphatase test revealed higher percentage (92%) of correctly identified environmental strains (n=127) than the ISO_LGMN procedure (83%) and proved to be a sensitive and reliable confirmation method.

Topics: Acid Phosphatase; Bacterial Proteins; Clostridium perfringens; Enzyme Assays; Feces; Gas Gangrene; Humans; Polymerase Chain Reaction; Water Microbiology

2011

Histochemical and electron microscopical studies of skeletal muscle affected by gas gangrene.

Folia histochemica et cytochemica, 1975, Volume: 13, Issue:3-4

Ultrastructural and histochemical changes of skeletal muscle were studied in three patients affected with gas gangrene. There was complete lack of the phosphorylase, succinate dehydrogenase and adenosine triphosphatase activities in the affected muscles of all the patients. In unaffected muscles these enzymes showed weaker activities than in norm. The lactate dehydrogenase activity, especially the heart type isozyme (LDH-1 or H4) proved less sensitive to the effect of clostridial toxin. A general increase in the acid phosphatase activity was found both in affected and in unaffected muscles. On electron microscopic examination damage to sarcolemmal membrane and disintegration of myofilaments was seen. The mitochondria were swollen and their cristae distorted and fragmented.

Topics: Acid Phosphatase; Adult; Female; Gas Gangrene; Histocytochemistry; Humans; Isoenzymes; L-Lactate Dehydrogenase; Male; Microscopy, Electron; Middle Aged; Mitochondria, Muscle; Muscles

1975