acid-phosphatase and Fibroma

The reactivity of lysosomal enzymes in 17 fibrohistiocytic tumors were examined histochemically to evaluate the histiocytic nature of the tumors. The lysosomal enzymes examined were acid phosphatase (Ac-P), non-specific esterase (NS-E), beta-glucuronidase (beta-GL), and N-acetyl-beta-glucosaminidase (N-GA). The tumors examined were eight malignant fibrous histiocytomas (MFH) (i.e., five common types, two myxoid types, and one inflammatory type), one atypical fibroxanthoma of the skin (AFX), one dermatofibrosarcoma protuberans (DFSP), two giant cell tumors of the tendon sheath (GCT), and five dermatofibromas (DF). Seven of 8 MFH showed strong reactivity for all enzymes examined. Positive reactions were seen as evenly distributed granules in the cytoplasm of fibroblastic, histiocytic and giant cells in all types of the tumors. Almost all AFX, GCT, and DF showed moderate to strong reactivity. These findings suggest that these tumors were composed of cells with the enzymatic character of histiocytes. However, DFSP contained no cells giving a positive reaction, and thus its histiocytic nature could not demonstrated.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Carboxylesterase; Carboxylic Ester Hydrolases; Female; Fibroma; Fibrosarcoma; Glucuronidase; Histiocytoma, Benign Fibrous; Histocytochemistry; Humans; Lysosomes; Male; Middle Aged; Skin Neoplasms; Xanthomatosis

Three cases of infantile digital fibromatosis were studied by electron microscopy, enzyme histochemistry, and tissue culture. The tumors were made up equally of myofibroblasts containing electron-dense inclusions which were composed chiefly of microfilaments measuring about 5 to 7 nm. Dense bodies usually observable in the smooth muscle cells were found in the bundles of these microfilaments and in the process of the inclusions, suggesting that these inclusions may represent an abnormal accumulation of contractile protein in the cytoplasm of tumor cells. Two cell lines were established from culture of the tumor cells, and the cultured cells also contained inclusion bodies showing the same morphologic characteristics as those of the original tumor cells. Lysosomal enzymes were abundant in the cultured cells, but they were scant in the cells of the fresh tissue specimens. Cocultivation of the cultured cells with human embryonic lung cells yielded no cytopathic effect.

Topics: Acid Phosphatase; Culture Techniques; Female; Fibroma; Fingers; Histocytochemistry; Humans; Inclusion Bodies; Infant; Infant, Newborn; Infant, Newborn, Diseases; Male; Skin Neoplasms; Toes

Topics: Acid Phosphatase; Alkaline Phosphatase; Connective Tissue; Connective Tissue Cells; DNA; Enzymes; Esterases; Fibroma; Fibrosarcoma; Granulation Tissue; Histiocytoma, Benign Fibrous; Histocytochemistry; Humans; Leprosy; Leucyl Aminopeptidase; Lymphoma, Large B-Cell, Diffuse; Mycosis Fungoides; Skin; Skin Diseases; Skin Neoplasms; Tuberculosis, Cutaneous

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Cattle; Cattle Diseases; Citric Acid Cycle; Esterases; Fibroma; Histocytochemistry; Lipase; Orbit; Orbital Neoplasms; Papilloma; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Bone Neoplasms; Child; Child, Preschool; Esterases; Female; Femoral Neoplasms; Fibroma; Fibrosarcoma; Fibrous Dysplasia of Bone; Glucuronidase; Humans; Humerus; Hydrolases; Infant, Newborn; Leucyl Aminopeptidase; Male; Mandibular Neoplasms; Middle Aged; Osteosarcoma; Tibia

Topics: Acid Phosphatase; Animals; Animals, Newborn; Cell Line; Cells, Cultured; Clone Cells; Cricetinae; Culture Techniques; Esterases; Female; Fibroma; Glucuronidase; Hemangiosarcoma; Histocytochemistry; Injections, Subcutaneous; Lactation; Male; Neoplasm Transplantation; Pregnancy; Sex Factors

Topics: Acid Phosphatase; Acridines; Adenocarcinoma; Argon; Cell Line; Cell Nucleus; Colonic Neoplasms; Culture Techniques; Cytoplasm; Female; Fibroma; Histocytochemistry; Hot Temperature; Lasers; Lysosomes; Microscopy, Phase-Contrast; Neoplasms; Photosensitivity Disorders; Radiation Effects; Staining and Labeling; Uterine Neoplasms

Topics: Acid Phosphatase; Age Factors; Aged; Fibroma; Humans; Hyperplasia; Intubation, Intratracheal; Male; Methods; Middle Aged; Prostate; Prostatectomy; Prostatic Neoplasms; Prostatitis; Pulmonary Heart Disease; Tartrates; Urinary Catheterization; Urinary Diversion

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Female; Fibroma; Jaw Neoplasms; Male; Mice; Neoplasms, Radiation-Induced; Osteosarcoma; Pathology; Radioisotopes; Radium; Sarcoma, Experimental; Thorium

Topics: Acid Phosphatase; Biopsy; Female; Fibroblasts; Fibroma; Granuloma; Histiocytes; Histocytochemistry; Humans; Lysosomes; Male; Microscopy, Electron; Middle Aged; Povidone; Skin; Skin Diseases

Topics: Acid Phosphatase; Adolescent; Adult; Child; Child, Preschool; Esterases; Fibroma; Histocytochemistry; Humans; Infant; Karyometry; Laryngeal Neoplasms; Microscopy, Electron; Middle Aged; Pharyngeal Neoplasms

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Bone Neoplasms; Carboxylesterase; Child; Chondroblastoma; Chondroma; Chondrosarcoma; Coloring Agents; Esterases; Fibroma; Fibrosarcoma; Fibrous Dysplasia of Bone; Geriatrics; Giant Cell Tumors; Glucuronidase; Histocytochemistry; Histological Techniques; Humans; Osteosarcoma; Pathology; Phosphoric Monoester Hydrolases; Sarcoma, Synovial; Staining and Labeling

Topics: Acid Phosphatase; Adenoma; Alkaline Phosphatase; Ameloblastoma; Arthritis; Bone Cysts; Bone Neoplasms; Chondrosarcoma; Fibroma; Fibrous Dysplasia of Bone; Geriatrics; Giant Cell Tumors; Humans; Neoplasm Metastasis; Osteitis Fibrosa Cystica; Osteoma; Osteosarcoma; Prognosis; Radiography; Sarcoma, Ewing; Tuberculosis; Tuberculosis, Osteoarticular