acid-phosphatase and Eye-Burns

Activities of different enzymes (acid glycosidases, phosphatases, Na+ - K+ -dependent ATPase, proteases, dehydrogenases) and acid glycosaminoglycans were studied by histochemical methods in sections of rabbit anterior eye segments after experimental alkali burn and treatment with aprotinin, an inhibitor of plasmin and other serine proteinases. Solutions of sodium hydroxide (0.25-1.0 M) were applied on corneas using 12-mm-diameter plastic tube for 15-60 s. After wiping with cotton and rinsing with tap water aprotinin solutions were applied in saline (in experimental animals) and saline (in control animals) dropwise in 12-h intervals for a month. Within the first two weeks aprotinin was used at a concentration of 5000 IU/ml. During the subsequent two weeks the aprotinin concentration was reduced to 2500 IU/ml. Striking differences in enzyme activities and in the healing between treated and untreated eyes were found. Without aprotinin, ulcers developed in most corneas within 3 weeks and plasmin was regularly demonstrated in tears and in the aqueous. When aprotinin treatment was started within 24 h after the burn, the number of enzymatically active inflammatory cells was significantly lower, not only in the cornea itself but also in the whole anterior eye segment. With aprotinin treatment no ulcerations and no plasmin in tears and the aqueous were observed and the corneas healed within a month. The healing process started from the zone of enzymatically activated corneal cells in the unburned zone at the corneal periphery. In the regenerating epithelium and endothelium high activities of Na+ -K+ -dependent ATPase, gamma-glutamyltransferase, lactate and succinate dehydrogenases appeared very soon.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Aprotinin; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; Eye; Eye Burns; gamma-Glutamyltransferase; Glycoside Hydrolases; Histocytochemistry; L-Lactate Dehydrogenase; Rabbits; Sodium Hydroxide; Sodium-Potassium-Exchanging ATPase; Succinate Dehydrogenase; Wound Healing

Topics: Acid Phosphatase; Animals; Burns, Chemical; Caustics; Cornea; Eye Burns; Lysosomes; Rabbits; Time Factors

In order to assess the extent of injury and the response of the cornea to alkali burns, NaOH in concentrations of 0.5, 0.25, 0.1, 0.05, and 0.01 N was applied to rabbit eyes and the histologic and metabolic changes studied. The activities of alkaline and acid phosphatases in homogenates and in cold microtome sections were examined on days 1, 4, and 7 after injury. At all time intervals 0.5 N and 0.25 N NaOH induced a remarkable decrease in enzyme activities. On the other hand, after 0.05 N and 0.01 N NaOH only very slight changes were observed. Using 0.1 N NaOH, both phosphatases decreased on day 4 after treatment and acid phosphatase reached normal values in a week, whereas alkaline phosphatase increased with a maximum on day 7. Its role in synthetic processes during corneal regeneration is discussed. Both histologic and metabolic patterns in the experimentally burned cornea were shown to be a function of NaOH concentration and the duration of contact. The process of re-epithelization of the cornea during healing after 0.1 N NaOH for 1 min is described.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Cornea; Corneal Injuries; Eye Burns; Histocytochemistry; Phosphoric Monoester Hydrolases; Rabbits; Regeneration; Sodium Hydroxide

Alkaline and acid phosphatases were studied in the rabbit cornea following acetone and ethanol exposure to the eye. Changes in enzyme activities were investigated in homogenates of epithelium and stroma quantitatively and in frozen cryostat sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings showed a remarkable increase in alkaline phosphatase of the epithelium beginning on day 7. This activation persisted until day 28 after instillation of both noxae with maximum activity on day 14. However, acetone was proved to be significantly more effective than ethanol. In addition, a different topochemistry of alkaline phosphatase was found in the epithelium of treated corneas, i.e., enzyme activity was observed not only superficially but in all epithelial layers of the cornea as compared to a normal one. The effect of acetone and ethanol on a regenerating corneal epithelium is discussed.

Topics: Acetone; Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Cornea; Epithelium; Ethanol; Eye Burns; Histocytochemistry; Rabbits

Topics: Acetylcysteine; Acid Phosphatase; Animals; Burns, Chemical; Corneal Injuries; Cysteine; Eye Burns; Gels; L-Lactate Dehydrogenase; Ophthalmic Solutions; Rabbits

Topics: Acid Phosphatase; Animals; Cornea; Corneal Injuries; Eye Burns; Glucuronidase; Glycosaminoglycans; Lysosomes; Rabbits

Topics: Acid Phosphatase; Acids; Alkalies; Alkaline Phosphatase; Animals; Burns, Chemical; Eye Burns; Rabbits; Time Factors

Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Eye; Eye Burns; Hydrochloric Acid; Rabbits; Sodium Hydroxide