acid-phosphatase and Endometriosis

Although alterations in patterns of protein secretion revealed in uterine flushings from mares suffering from endometrosis have been described, little is known about alterations at the cellular level. Hence, the aim of this study was to characterize deviations in patterns of uterine gland secretion patterns using endometrial biopsies, histochemical and newly established immunohistochemical methods. Forty-eight endometrial biopsies were obtained from mares suffering from various types of endometrosis (active and inactive, destructive and non-destructive) and degree (mild to severe) were analyzed for expression of the proteins uteroglobin, uteroferrin, calbindinD9k and uterocalin as representatives of endometrial proteins detectable by immunohistochemistry using polyclonal antibodies. Glycogen was identified using the PAS-reaction and mucopolysaccharides were stained with alcian blue. Uterine glandular epithelia within fibrotic foci mostly revealed a protein and carbohydrate pattern of expression which was independent of hormonal changes during the estrous cycle. In comparison to non-affected glands, most epithelial cells within periglandular fibrosis exhibited decreased immunostaining intensity for proteins, especially when there was destructive endometrosis. However, uteroferrin staining intensity was strong within areas of severe destructive endometrosis. Moreover, only few basal glandular epithelial cells, especially those in cystic glands, stained for mucopolysaccharides that are typically seen within the luminal epithelia. Usually a single fibrotic focus caused only slight alterations in glandular proteins and carbohydrate reaction patterns, so that only more severe endometrosis lead to deviations which were detectable in uterine flushings. The highly sensitive methods used in the present study allow studies of uterine secretion patterns in the context of routine assessment of endometrial biopsies.

Topics: Acid Phosphatase; Animals; Calbindins; Carbohydrate Metabolism; Endometriosis; Endometrium; Female; Gene Expression Profiling; Gene Expression Regulation; Glycogen; Glycosaminoglycans; Horse Diseases; Horses; Immunohistochemistry; Isoenzymes; Lipocalins; Proteins; S100 Calcium Binding Protein G; Tartrate-Resistant Acid Phosphatase; Uteroglobin

Recent studies suggest that acid phosphatase locus 1 (ACP1) could be involved in T-cell antigen receptor signaling and in immune disorders. The present study shows that the ACP1( *)C allele, which is associated with elevated enzymatic activity, is significantly more common in women with endometriosis than in healthy women, but is less common in allergic than in nonallergic subjects. These findings suggest that carriers of high activity ACP1 genotypes are more susceptible to endometriosis but less susceptible to allergic manifestations than carriers of other ACP1 genotypes.

Topics: Acid Phosphatase; Adult; Causality; Comorbidity; Endometriosis; Female; Genetic Predisposition to Disease; Heterozygote; Humans; Hypersensitivity; Incidence; Polymorphism, Single Nucleotide; Risk Assessment; Risk Factors

The effects of estrogen suppression on osteonal remodeling in young women was investigated using transiliac biopsies (eight paired biopsies + four single pre; three single post biopsies) taken before and after treatment for endometriosis (6 months) with analogs of gonadotrophin releasing hormone (GnRH). Estrogen withdrawal increased the proportion of Haversian canals with an eroded surface (106%, p = 0.047), a double label (238%, p = 0.004), osteoid (71%, p = 0.002), and alkaline phosphatase (ALP) 116%, p = 0.043) but not those showing tartrate-resistant acid phosphatase (TRAP) activity (p = 0.25) or a single label (p = 0.30). Estrogen withdrawal increased TRAP activity in individual osteoclasts in canals with diameters greater than 50 microns (p = 0.0089) and also the number of osteons with diameters over 250 microns (p = 0.049). ALP activity in individual osteoblasts was increased but not significantly following treatment (p = 0.051). Wall thickness was significantly correlated with osteon diameter (p < 0.001). In a separate group of patients (four pairs + one post biopsy) on concurrent treatment with tibolone, there was no significant increase in the osteon density, cortical porosity, median canal diameter, or the markers of bone formation and resorption. Enzyme activities and numbers of active canals were also not increased with the concurrent treatment, but there was still an increase in the osteon diameter. As previously shown for cancellous bone, estrogen withdrawal increased cortical bone turnover. We have now shown that resorption depth within Haversian systems was also increased with treatment. The enhanced TRAP activity in individual osteoclasts supports the concept that osteoclasts are more active following estrogen withdrawal in agreement with theoretical arguments advanced previously. Understanding the cellular and biochemical mechanisms responsible for increased depth of osteoclast resorption when estrogen is withdrawn may allow the development of new strategies for preventing postmenopausal bone loss.

Topics: Acid Phosphatase; Alkaline Phosphatase; Biomarkers; Biopsy; Bone Density; Bone Remodeling; Drug Therapy, Combination; Endometriosis; Estrogen Antagonists; Female; Gonadotropin-Releasing Hormone; Goserelin; Humans; Ilium; Isoenzymes; Norpregnenes; Osteoclasts; Osteoporosis, Postmenopausal; Software; Tartrate-Resistant Acid Phosphatase; Triptorelin Pamoate

TO measure peripheral, axial, and total bone mass and to assess markers of bone remodeling in women with endometriosis, with the aim of addressing previous reports of diminished peripheral bone mass in these patients.. Whole body bone densitometry, estradiol (E2) levels, and biochemical bone markers (calcium, phosphorus, total alkaline phosphatase, tartrate-resistant acid phosphatase, and total proteins) were determined in 28 patients with endometriosis and compared with findings in 33 controls.. There were no significant differences between the groups in bone mass at different sites or in biochemical bone markers. We observed a significant negative correlation between tartrate-resistant acid phosphatase and E2 levels (P < .001) and with total (P < .001), head (P < .01), and axial (trunk) (P < .001) bone mass. Total alkaline phosphatase did not correlate with any of the indices studied.. Bone mass was not lower in any of the areas studied in women with endometriosis. There was a significant negative correlation of tartrate-resistant acid phosphatase with E2 and with total, head, and trunk bone mass.

Topics: Absorptiometry, Photon; Acid Phosphatase; Adult; Alkaline Phosphatase; Bone Density; Bone Remodeling; Endometriosis; Estradiol; Female; Humans

The authors reviewed the histologic slides of 2600 prostatic carcinomas seen at Memorial Hospital from 1963 to 1983. In ten cases, resection specimens had a predominantly endometrioid appearance. Six patients had polypoid lesions in and around the verumontanum, and one had a polypoid lesion away from the verumontanum. Two patients had no mucosal lesions and one was not cystoscoped. Histologically, the tumors showed a tall pseudostratified columnar epithelium, usually with amphophilic cytoplasm. The cells were arranged either along papillae or in complexes of large acini or in single glands. In eight of the ten cases, the endometrioid carcinomas were associated with a prior or coexistent typical microacinar prostatic adenocarcinoma. In four cases, the endometrioid pattern existed in a pure form, although in two such cases with urethral tumors, the patients had histories of successfully treated microacinar adenocarcinomas of the posterior prostatic lobe. In one case, a urethral endometrioid tumor coexisted with a small posterior lobe microacinar adenocarcinoma. In five cases, both endometrioid and microacinar carcinomas were seen, including endometrioid and microacinar carcinomas found at the same site at different times (2 cases), tumors with a predominantly endometrioid, yet focally microacinar pattern (1 case), and primary tumors where lymph node metastases had different histologic features (2 cases). Of the three patients with a pure or predominantly endometrioid pattern treated with diethylstilbestrol, two had a marked clinical response. All ten endometrioid prostatic adenocarcinomas showed prostate-specific antigen and prostate-specific acid phosphatase immunoreactivity, in contrast to none of the control uterine endometrial carcinomas. In material spanning a 20-year period, the authors have not seen a single prostatic tumor entirely analogous to the uterine endometrial carcinoma. Until such proof exists, prostatic carcinomas with endometrioid features are best classified and treated as variants of prostatic duct carcinomas.

Topics: Acid Phosphatase; Adenocarcinoma; Aged; Antigens, Neoplasm; Endometriosis; Follow-Up Studies; Histocytochemistry; Humans; Male; Middle Aged; Prostate-Specific Antigen; Prostatic Neoplasms

Peritoneal fluid from 35 women with endometriosis and from 34 control women was aspirated at laparoscopy and analyzed. No differences in prostanoid levels were found. The peritoneal fluid volume, macrophage concentration, macrophage content, and content of activated macrophages as measured by acid phosphatase staining were all significantly elevated in the endometriosis patients. The macrophages were incubated and the medium was added to the zona-free hamster egg sperm penetration assay. This medium caused a significant decrease in the percentage of ova penetrated in this assay. It is postulated that one of the mechanisms of infertility in women with endometriosis may involve the increased number of activated macrophages and their ability to interfere with sperm-egg interaction.

Topics: 6-Ketoprostaglandin F1 alpha; Acid Phosphatase; Ascitic Fluid; Cell Count; Dinoprost; Endometriosis; Female; Humans; Ibuprofen; Infertility, Female; Laparoscopy; Macrophage Activation; Macrophages; Male; Menstrual Cycle; Prostaglandins E; Prostaglandins F; Radioimmunoassay; Sperm-Ovum Interactions; Thromboxane B2

In 132 patients suffering from various malignancies, including uterine carcinoma, breast cancer, gastric carcinoma, cancer of the colon, and additionally, patients with uterine myomas and endometriosis an increased activity of AP within the peripheral blood neutrophils could be stated by using a histochemical method. According to the author's opinion an increased activity of that enzyme reflects a response against local inflammatory processes frequently accompanying malignant tumours.

Topics: Acid Phosphatase; Breast Neoplasms; Colonic Neoplasms; Endometriosis; Female; Humans; Leiomyoma; Male; Neoplasms; Neutrophils; Stomach Neoplasms; Uterine Neoplasms

Cellular components in peritoneal fluid of infertile patients with and without endometriosis were evaluated in 102 patients with Wright's-Giemsa and Papanicolaou stains. The secretory activity of these cells was studied indirectly by assaying acid phosphatase, prostaglandin (PG) F2 alpha and PGE2 and complement components C3c and C4. The results showed that macrophages and lymphocytes were the dominant cells in peritoneal fluid of these patients. These cells were significantly increased in endometriosis patients, as compared with control subjects. In addition, peritoneal fluid acid phosphatase, PGF2 alpha and PGE2, and complement components C3c and C4 were significantly increased in patients with endometriosis. These cellular changes and their activation in peritoneal fluid may explain infertility associated with endometriosis.

Topics: Acid Phosphatase; Adolescent; Adult; Ascitic Fluid; Complement C3; Complement C3c; Complement C4; Dinoprost; Dinoprostone; Endometriosis; Fallopian Tube Patency Tests; Female; Humans; Infertility, Female; Lymphocytes; Macrophages; Peritoneal Neoplasms; Prostaglandins E; Prostaglandins F; Staining and Labeling

Pelvic fluid was collected from 66 women undergoing laparoscopic sterilization or diagnostic laparoscopy for evaluation of infertility. Cells consisting mainly of macrophages were separated, counted, and subjected to histochemical staining for acid phosphatase and myeloperoxidase as markers of cell irritation. Pelvic fluid was analyzed for acid phosphatase, neutral protease, and extractable prostaglandin E2 and F2 alpha. A higher proportion (46% versus 15%) of the macrophages in the group with mild endometriosis exhibited positive staining for acid phosphatase as compared with the fertile group. Pelvic fluid from patients with mild endometriosis had higher acid phosphatase and neutral protease activity than that from fertile patients (p less than 0.05, p less than 0.01). The content of either prostaglandin was not significantly higher in the endometriosis group as compared with the fertile group. The results suggest that mild endometriosis is associated with activation of macrophages and release of active substances into peritoneal fluid that may be responsible for the associated infertility.

Topics: Acid Phosphatase; Dinoprost; Dinoprostone; Endometriosis; Endopeptidases; Female; Humans; Infertility, Female; Macrophages; Neprilysin; Pelvic Neoplasms; Prostaglandins E; Prostaglandins F

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Alkaline Phosphatase; Connective Tissue; Endometriosis; Female; Histocytochemistry; Humans; Ovarian Neoplasms; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Alkaline Phosphatase; Endometriosis; Endometrium; Female; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Succinate Dehydrogenase

Topics: Abortion, Habitual; Acid Phosphatase; Alkaline Phosphatase; DNA; Endometriosis; Endometrium; Female; Glycogen; Histocytochemistry; Humans; Hyperplasia; Menopause; Pregnancy; Proteins; RNA