acid-phosphatase and Endometrial-Hyperplasia

The mononuclear cells in the endometrial stoma change in reactivity for lysosomal hydrolases during the menstrual cycle. Lymphoid follicles may occur in the stroma in any phase of the cycle and have been found in gestational endometrium. However, these cells have no significant lysosomal activity. Alterations in the endometrium are reflected in modified patterns of activity. Endometritis, association with an intrauterine contraceptive device, pregnancy, and adenocarcinoma result in increased numbers and staining intensity of mononuclear cells. In contrast, no consistent changes were apparent in foci of glandular hyperplasia, and decreased staining was seen in atrophic areas of endometrium. These data suggest that interstitial mononuclear cells are a sensitive monitor of morphologic changes in the endometrium.

Topics: Acid Phosphatase; Adenocarcinoma; Atrophy; Endometrial Hyperplasia; Endometritis; Endometrium; Esterases; Female; Galactosidases; Glucuronidase; Hexosaminidases; Histocytochemistry; Humans; Hydrolases; Hyperplasia; In Vitro Techniques; Intrauterine Devices; Leucyl Aminopeptidase; Lysosomes; Menstruation; Pregnancy; Uterine Neoplasms

The purpose of this study was to evaluate the endometrial acid and alkaline phosphatase activity in rhesus monkeys chronically treated for 24 months with 3 levels of quinestrol (17-ethynlestradiol cyclopentyl ether). The 3 levels were: 1) 4 animals, 4 mcg/kg;2) 3 animals, 20mcg/kg; 3) 4 animals 100 mcg/kg. There were 4 animals included as sesame oil controls. All monkeys receiving quinestrol had some degree of cystic endometrial hyperplasia, dose-related, ranging from trace, to weak, to moderate cystic endometrial hyperplasia for high dose animals. The treated monkeys also showed thickening of the glandular basement membrane and an increase in endometrial and myometrial collagen (fibrosis), also apparently dose-related. In the monkeys receiving quinestrol, glandular alkaline phosphatase activity was comparable to that of the follicular and early luteal animals while the acid enzyme activity resembled that found in early and late luteal controls. Frequency of menstrual cycles was reduced.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Endometrial Hyperplasia; Estranes; Estrus; Female; Haplorhini; Histocytochemistry; Intubation, Gastrointestinal; Pregnancy; Time Factors; Uterus

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Animals; Disease Models, Animal; Endometrial Hyperplasia; Endometrium; Estradiol; Female; Histocytochemistry; Microscopy, Electron; Neoplasms, Experimental; Rabbits; Uterine Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Cervix Uteri; Dihydrolipoamide Dehydrogenase; Endometrial Hyperplasia; Endometrium; Esterases; Female; Glucosephosphate Dehydrogenase; Glucuronidase; Histocytochemistry; Humans; Monoamine Oxidase; Oxidoreductases; Uterine Cervical Diseases; Uterine Diseases; Uterine Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Biopsy; Carboxylesterase; Endometrial Hyperplasia; Endometrium; Esterases; Female; Humans; Neoplasms; Pathology; Research; Uterine Neoplasms