acid-phosphatase and Duodenal-Ulcer

Topics: Acid Phosphatase; Acute Disease; Adult; Aged; Alkaline Phosphatase; Cathepsin B; Cathepsin C; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; Duodenal Ulcer; Humans; Leukocytes; Lysosomes; Middle Aged; Peptic Ulcer Hemorrhage; Stomach Ulcer

In examination of 51 patient with acute gastrointestinal bleeding of ulcer etiology, the activity of lysosomal enzymes, peroxide oxidation of lipids were increased, the activity of antioxidant system was decreased. This can lead to inhibition of proliferative processes, stipulating the severity of blood loss, course of the postoperative period, the incidence of postoperative complications, and lethality.

Topics: Acid Phosphatase; Acute Disease; Adult; Aged; Cathepsin D; Deoxyribonucleases; Duodenal Ulcer; Enzyme Activation; Humans; Lipid Peroxidation; Lysosomes; Middle Aged; Peptic Ulcer Hemorrhage; Ribonucleases; Stomach Ulcer

Cysteamine and propionitrile cause severe duodenal ulcers with perforation within 24-48 h after a single injection in rats. These animal models were used to gain insight into the early, preulcerogenic biochemical changes in the duodenal mucosa. The results indicate that a single sc injection of cysteamine and propionitrile induced dose- and time-dependent decreases in the activity of phosphoprotein phosphatase (PPPase) in homogenate and particulate fractions of rat duodenal mucosa. The decrease in enzyme activity was detectable 4 h after the injection of the ulcerogens, it was maximal at 12 h, and hardly detectable at 24 h. No effect on the enzyme activity was found under in vitro conditions. PPPase activity in the liver was not influenced by either cysteamine or propionitrile. Furthermore, the toxic but nonulcerogenic derivative of cysteamine ethanolamine had no effect on PPPase in the duodenum. Thus, the effect of the duodenal ulcerogens on PPPase activity was indirect and organ specific, related only to the target organ (i.e., duodenal mucosa). The effect of the drugs was also selective at the level of mucosal cells: both duodenal ulcerogens depleted protein and alkaline phosphatase but not lysosomal acid phosphatase. The decrease of PPPase activity could be a general property of the duodenal ulcerogens since it is independent of their effect on endogenous somatostatin.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cysteamine; Dose-Response Relationship, Drug; Duodenal Ulcer; Duodenum; Ethanolamine; Ethanolamines; Female; Intestinal Mucosa; Kinetics; Nitriles; Phosphoprotein Phosphatases; Rats; Rats, Inbred Strains; Somatostatin

Peculiar myelin figures were abundantly found in some basal-granulated cells including S, D1 and I cells in human Brunner's glands. Intense acid phosphatase activity was found in the periphery of the myelin figures, indicating that they were secondary lysosomes or residual bodies. The acid phosphatase activity was also found in some secretory granules. There were some secretory granules which were partly membranous in content, suggesting the initial stage of their degradation into myelin figures. There were also features indicating the fusion of secretory granules with the myelin figures. All these findings suggest that the myelin figures are the products of lysosomal degradation of secretory granules. The rate of occurrence of basal-granulated cells containing myelin figures in Brunner's glands tended to be higher in subjects with duodenal ulcer than in cases of gastric cancer or ulcer.

Topics: Acid Phosphatase; Adult; Aged; Brunner Glands; Cytoplasmic Granules; Duodenal Ulcer; Duodenum; Humans; Lysosomes; Male; Microscopy, Electron; Middle Aged

Experiments were performed on white rats with experimental acetate ulcer of the stomach and intestine. At the height of destruction of gastric mucosa tissue (60 minutes from the onset of ulcer formation) a consistent increase in acid phosphatase secretion was recorded. This process was accompanied, as a rule, by significant elevation of proteolytic activity (pH 6.5-7.0) in tissue extract obtained from the affected area. It is suggested that the increased proteolytic activity observed at pH 6.5-7.0 in the tissue extract from the ulcerous area is most likely related to the release of lysosomal proteases at the height of tissue destruction. It thus appears that ulcer of the gastric mucosa induced by acetic acid application is determined by interstitial proteolytic activity of cathepsins that are released during the destruction of lysosomal membranes.

Topics: Acid Phosphatase; Animals; Duodenal Ulcer; Female; Gastric Mucosa; Hydrogen-Ion Concentration; Male; Peptide Hydrolases; Rats; Stomach Ulcer

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Clinical Enzyme Tests; Duodenal Ulcer; Humans; Peptic Ulcer; Periodontal Diseases; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Adenosine Monophosphate; Duodenal Ulcer; Electrophoresis, Starch Gel; Erythrocytes; Humans; Intestinal Mucosa; Isoenzymes; Jejunum; Phenotype; Phosphotransferases; Polymorphism, Genetic

Topics: Acid Phosphatase; Alkaline Phosphatase; Autopsy; Burns; Cholinesterases; Colonic Diseases; Duodenal Ulcer; Esophagitis, Peptic; Gallbladder Diseases; Gastric Mucosa; Glycosaminoglycans; Histocytochemistry; Humans; Mucins; Nucleoproteins; Pharyngeal Diseases; Stomach Ulcer; Ulcer

Topics: Acetylcholinesterase; Acid Phosphatase; Adult; Aged; Alanine Transaminase; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Cholinesterases; Diagnosis, Differential; Duodenal Ulcer; Humans; Hydrolases; Middle Aged; Stomach Neoplasms; Stomach Ulcer; Transaminases

Topics: Acid Phosphatase; Adenocarcinoma; Aged; Biopsy; Carcinoma; Diethylstilbestrol; Duodenal Ulcer; Erectile Dysfunction; Humans; Male; Methods; Middle Aged; Neoplasm Metastasis; Palpation; Postoperative Complications; Prognosis; Prostatectomy; Prostatic Neoplasms; Spinal Neoplasms; Urinary Tract Infections; Urine

Topics: Acid Phosphatase; Animals; Dogs; Duodenal Ulcer; Gastric Juice; Gastric Mucosa; Histamine; Histocytochemistry; Oxidoreductases; Pyrophosphatases; Radiation Effects

Topics: Acid Phosphatase; Agar; Antigen-Antibody Reactions; Antigens; Catalase; Duodenal Ulcer; Electrophoresis; Esterases; Gastrectomy; Gastric Mucosa; Glycoproteins; Humans; Immunoelectrophoresis; L-Lactate Dehydrogenase; Peroxidases

Topics: Acid Phosphatase; Duodenal Ulcer; Duodenum; Gastric Acidity Determination; Gastric Juice; Histamine; Humans; Hydrochloric Acid; Male; Pharmacology; Stomach