acid-phosphatase and Diabetes-Mellitus

Topics: Acetylglucosaminidase; Acid Phosphatase; Animals; Arteries; Arteriosclerosis; Diabetes Mellitus; Endocytosis; Humans; Hypertension; Lipid Metabolism; Lysosomes; Rabbits

Topics: Acid Phosphatase; Alkaline Phosphatase; Amylases; Blood Glucose; Cholinesterases; Diabetes Mellitus; Diabetic Coma; Glucose-6-Phosphate Isomerase; Glycoside Hydrolases; Humans; Lysosomes; Time Factors; Trehalase

Topics: Acid Phosphatase; Animals; Citric Acid Cycle; Diabetes Mellitus; Glucosephosphate Dehydrogenase; Hexokinase; Histocytochemistry; Humans; Insulin; Islets of Langerhans; Malate Dehydrogenase; Mice; Pancreas; Phosphoglucomutase; Phosphogluconate Dehydrogenase; Pyruvate Kinase; Rabbits; Rats; Transaminases

Topics: Acid Phosphatase; Adult; Animals; Citric Acid Cycle; Diabetes Mellitus; Erythroblastosis, Fetal; Female; Glycogen; Glycolysis; Histocytochemistry; Humans; Hyperglycemia; Infant, Newborn; Insulin; Islets of Langerhans; Lysosomes; Mice; Obesity; Pentosephosphates; Pregnancy; Transaminases

To assess the effect of topical application of melatonin to the gingiva on salivary fluid concentrations of acid phosphatase, alkaline phosphatase, osteopontin, and osteocalcin.. Cross-sectional study of 30 patients with diabetes and periodontal disease and 30 healthy subjects. Diabetic patients were treated with topical application of melatonin (1% orabase cream formula) once daily for 20 days and controls with a placebo formulation.. Before treatment with melatonin, diabetic patients showed significantly higher mean salivary levels of alkaline and acid phosphatase, osteopontin and osteocalcin than healthy subjects (P < 0.01). After treatment with melatonin, there was a statistically significant decrease of the gingival index (15.84 ± 10.3 vs 5.6 ± 5.1) and pocket depth (28.3 ± 19.5 vs 11.9 ± 9.0) (P < 0.001). Also, use of melatonin was associated with a significant reduction of the four biomarkers. Changes of salivary acid phosphatase and osteopontin correlated significantly with changes in the gingival index, whereas changes of alkaline phosphatase and osteopontin correlated significantly with changes in the pocket depth.. Treatment with topical melatonin was associated with an improvement in the gingival index and pocket depth, a reduction in salivary concentrations of acid phosphatase, alkaline phosphatase, osteopontin and osteocalcin.

Topics: Acid Phosphatase; Administration, Topical; Adult; Aged; Alkaline Phosphatase; Cross-Sectional Studies; Diabetes Mellitus; Female; Gingiva; Humans; Male; Melatonin; Middle Aged; Osteocalcin; Osteopontin; Periodontal Diseases; Periodontal Index; Saliva

Salivary components may suffer variations that can be detected by chemical determinations. The aim of this work was to determine physical and biochemical characteristics of the saliva of a group of diabetic children compared to those of a control group. Relation to oral health indices was also determined. Twenty diabetic children (3-15-years-old) and 21 control children (5-12-years-old) were included in this study. Total proteins, sugars and calcium were determined by colorimetric methods, and glucose, urea, alpha-amylase and acid phosphatase by enzymatic methods. Our results demonstrated that acidic pH, diminished salivary flow rate and excess foam are usually present in saliva of diabetic children. Total sugars, glucose, urea and total proteins were greater in diabetic patients than controls, while calcium values were decreased. These differences were confirmed by the discrimination test. Diabetic children have higher DMFT-dmft-deft and DMFS-dmfs-defs values compared to those of the control children despite their lower sugar intake. Some salivary components in addition to the diminished flow rate could be involved in the characterization of the oral health state of diabetic children.

Topics: Acid Phosphatase; Adolescent; alpha-Amylases; Analysis of Variance; Calcium; Carbohydrates; Child; Child, Preschool; Colorimetry; Dental Plaque Index; Diabetes Mellitus; DMF Index; Female; Glucose; Humans; Hydrogen-Ion Concentration; Linear Models; Male; Periodontal Index; Saliva; Salivary Proteins and Peptides; Secretory Rate; Urea

We have studied a new sample of 276 NIDDM patients from the population of Penne (Italy). Comparison of the new data with those of 214 diabetic pregnant women from the population of Rome reported in a previous paper has shown that the pattern of association between low molecular weight acid phosphatase genotype and degree of glycemic control is similar in the two classes of diabetic patients. Among nonobese subjects the proportion of ACP1*A (the allele showing the lowest enzymatic activity) is lower in diabetic patients with high glycemic levels (mean value greater than 8.9 mmol/l) than in diabetic patients with a low glycemic level (mean value less than 8.9 mmol/l). Among obese subjects no significant association is observed between glycemic levels and ACP1. Among nonobese subjects the concentration of f isoform of ACP1 is higher in patients showing a high glycemic level than in patients showing a low glycemic level. No significant difference is observed for s isoform.

Topics: Acid Phosphatase; Adult; Aged; Aged, 80 and over; Blood Glucose; Diabetes Mellitus; Diabetes Mellitus, Type 2; Female; Genotype; Humans; Isoenzymes; Male; Middle Aged; Molecular Weight; Obesity; Pregnancy; Pregnancy in Diabetics

The cells of Burkholderia pseudomallei, B. cepacia and Pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. The former two species were stained positively indicating insulin binding but P. aeruginosa was not. Insulin exposure reduced phospholipase C and acid phosphatase activities of B. pseudomallei but did not affect those enzymatic activities of B. cepacia in the employed experimental conditions. It is suggested that B. pseudomallei have insulin receptors which may be associated with a signal transfer system involving phospholipase and protein tyrosine phosphatase.

Topics: Acid Phosphatase; Burkholderia cepacia; Burkholderia Infections; Burkholderia pseudomallei; Diabetes Complications; Diabetes Mellitus; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Insulin; Type C Phospholipases

Possible selective interaction between genetic polymorphisms of acid phosphatase locus 1 (ACP1) and adenosine deaminase (ADA) has been investigated in a sample of 211 infants from diabetic women, and in 350 consecutive infants from normal women. Newborns from diabetic pregnancies carrying the ADA2 allele show a lower proportion of BA and CB phenotypes (heterozygotes for the main allele of ACP1 system), compared with both their mothers and normal infants. The observation suggests that, in a diabetic environment, intrauterine selection may act against double heterozygotes for the ACP1 and ADA systems.

Topics: Acid Phosphatase; Adenosine Deaminase; Birth Weight; Diabetes Mellitus; Female; Fetus; Gene Expression Regulation; Humans; Phenotype; Polymorphism, Genetic; Pregnancy; Pregnancy in Diabetics

Platelets were isolated from blood donated by 57 diabetic subjects, 41 insulin-dependent and 16 non insulin-dependent, ranging in age from 19 to 78 years, and by 54 healthy non-diabetic subjects ranging from 19 to 63 years of age. The platelets were ruptured by sonication and resultant preparations assayed for their levels of activity of seven acid glycohydrolases. Platelets from diabetic subjects contained only 50% of the alpha-L-fucosidase activity and about 60% of the acid phosphatase, beta-D-galactosidase, and beta-D-glucosidase activities of platelets from non-diabetic individuals; the differences were statistically significant. N-acetyl-beta-D-glucosaminidase activity in platelets from diabetic subjects was reduced by about 15% from normal levels while beta-D-glucuronidase and alpha-D-mannosidase activities were similar to those from non-diabetic individuals. A comparison of the data from older insulin-dependent diabetic and normal subjects with a similar age distribution yielded identical results for the two groups in all enzymes tested except fucosidase. Platelets of non insulin-dependent diabetics and those from non-diabetic subjects of a similar age distribution appear to possess similar levels of these acid hydrolases. There was no difference in levels of these platelet acid hydrolases between males and females in either the diabetic or non-diabetic group. Within the diabetic group, there was no difference in these platelet acid hydrolase activities between subjects with retinopathy and without retinopathy. There was no correlation of the enzyme activity levels of platelets from diabetic subjects with concentration of glycosylated haemoglobin, serum triglycerides or serum cholesterol.

Topics: Acid Phosphatase; Adult; Aged; Blood Platelets; Diabetes Mellitus; Female; Glycated Hemoglobin; Glycoside Hydrolases; Humans; Male; Middle Aged

Cytochemical studies have been performed on peripheral blood lymphocytes of 68 diabetic subjects, with various conditions of metabolic control, and 15 newly diagnosed insulin-dependent diabetic patients. 20 patients of the 1 group had diabetic retinopathy. In diabetic patients periodic acid Schiff positivity, acid phosphatase, and N-acetyl-beta-glucosaminidase activities of lymphocytes are fairly impaired, particularly in insulin-dependent diabetes. Concerning the alpha-naphthyl-acetate-esterase activity, the percentage of positive cells with coarse granules is significantly reduced (p less than 0.001) in diabetic patients as compared to controls, without difference related to age and sex. These abnormalities are more evident in patients with poor glyco-metabolic control. In patients with newly diagnosed insulin-dependent diabetes we have found a further decrease in alpha-naphthyl-acetate-esterase activity, and an increase in acid phosphatase and N-acetyl-beta-glucosaminidase activities. Cyto-enzymatic activities are not significantly different in subjects with diabetic retinopathy. The results of peripheral lymphocyte enzymatic activities in diabetics could be related to a depression of the cell-mediated immunity and could enhance the infections risk of these patients. Furthermore our data show an altered immunological balance in subjects with newly diagnosed type I diabetes.

Topics: Acid Phosphatase; Adult; Aged; Diabetes Mellitus; Diabetes Mellitus, Type 1; Diabetic Retinopathy; Female; Hexosaminidases; Histocytochemistry; Humans; Lymphocytes; Male; Middle Aged; Naphthol AS D Esterase; Periodic Acid-Schiff Reaction

Cytochemical indices of leukocytes were determined in 16 patients with diabetes mellitus in the period of unbalancing and balancing. The following tests were made: content of glycogen and lipids, acid phosphatase (AP), alkaline phosphatase (AIP), myeloperoxidase (MPO) and nonspecific alpha-naphtol acetate esterase (NANAE) activity. In unbalanced diabetics an evident decrease in the activity of AP and MPO could be noted as well as a decrease of glycogen content and an increase of lipid content. An insignificant decrease could be observed in the activity of ALP and NANAE in granulocytes. A slight increase in the activity of NANAE in monocytes would be found. Balancing this disease induced the increase of all parameters in granulocytes except MPO activity. It is interesting to note that balancing diabetes mellitus deepened the observed changes in the decrease or increase of tested parameters. The presented findings clearly indicate the role of metabolic disorders in diabetes mellitus on the activity of some neutrophilic enzymes and the glycogen and the content of lipids in neutrophils.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Diabetes Mellitus; Female; Glycogen; Humans; Leukocytes; Lipids; Male; Middle Aged; Naphthol AS D Esterase; Peroxidase

Topics: Acid Phosphatase; Administration, Oral; Adult; Aged; Biguanides; Diabetes Mellitus; Female; Humans; Insulin; Lymphocytes; Male; Middle Aged; Sulfonylurea Compounds

Topics: Acid Phosphatase; Adult; Aged; Diabetes Complications; Diabetes Mellitus; Female; Humans; Infections; Lymphocytes; Male; Middle Aged

In 55 patients with diabetes mellitus metabolically balanced the activity and intracellular localization of acid phosphatase has been estimated by means of semiquantitative method of Barka and Anderson [3] in peripheral blood lymphocytes. It has been shown that patients in question, both insulin-dependent and those with overweight, in comparison to control group, exhibit more than twofold lower numbers of lymphocytes having intact enzyme positive lysosomes and more than twofold higher numbers of that cells with damaged lysosomes and extralysosomal localisation of the enzyme. According to authors suggestion the above changes may be associated with disturbed lymphocyte functions in the patients with diabetes mellitus.

Topics: Acid Phosphatase; Adult; Aged; Diabetes Mellitus; Female; Humans; Leukocyte Count; Lymphocytes; Lysosomes; Male; Middle Aged

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Blood Proteins; Diabetes Mellitus; Female; Glycogen; Humans; Leukocytes; Male; Middle Aged; Nucleic Acids; Phagocytosis

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Diabetes Mellitus; Female; Glucuronidase; Glycogen; Histocytochemistry; Humans; Lipids; Male; Middle Aged; Neutrophils; Peroxidase

Topics: 2',3'-Cyclic-Nucleotide Phosphodiesterases; Acid Phosphatase; Animals; beta-Glucosidase; Brain; Diabetes Mellitus; Diabetes Mellitus, Experimental; Glucose-6-Phosphatase; Hexosaminidases; Humans; Male; Peptide Hydrolases; Peripheral Nerves; Rats; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Animals; Child; Diabetes Mellitus; Diabetes Mellitus, Experimental; Glucose-6-Phosphatase; Humans; L-Iditol 2-Dehydrogenase; L-Lactate Dehydrogenase; Middle Aged; Rabbits

Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaline Phosphatase; alpha-L-Fucosidase; Aspartate Aminotransferases; Diabetes Mellitus; Diuresis; Galactosidases; Hydrolases; Insulin; Mannosidases; Streptozocin

In a complex investigation were evidenced 12 selected enzymes (oxidoreductases and hydrolases) in the pancreas of normoglycemic sand rats (Psammomys obesus). The sand rat developed a spontaneous diabetic metabolism by holding in the vivarium. The material based of animal groups that were fooded ad libitum or after defined dietetic regimes. The topochemical pattern of enzymes in the Langerhans' islets and exocrine acini can be compared between the sand rat groups and with the good investigated model of the Wistar rat. Because enzymhistochemical investigations of the sand rat pancreas absented, were the results compared with the rare specific observations of other animals and the man and the changed metabolic level were discussed under the especially food situation and in diabetes mellitus.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alcohol Oxidoreductases; Animals; Diabetes Mellitus; Diet; Energy Intake; Glucose-6-Phosphatase; Histocytochemistry; Islets of Langerhans; Pancreas; Rats; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adipose Tissue; Animals; Diabetes Mellitus; Epididymis; Galactosidases; Glucosidases; Glycoside Hydrolases; Hexosaminidases; Kidney; Liver; Lysosomes; Male; Rats; Spleen; Streptozocin; Testis

Topics: Acid Phosphatase; Adult; Alanine Transaminase; Aspartate Aminotransferases; Blood Glucose; Diabetes Mellitus; Diabetic Angiopathies; Enzyme Activation; Female; Humans; Insulin; Lysosomes; Male; Middle Aged; Spectrophotometry

Topics: Acid Phosphatase; Alkaline Phosphatase; Biopsy; Diabetes Mellitus; Fatty Liver; Glutamate Dehydrogenase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Liver; Liver Diseases; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Biliary Tract Diseases; Cholecystectomy; Cholelithiasis; Chronic Disease; Colitis, Ulcerative; Diabetes Mellitus; Duodenum; Gastrectomy; Gastritis; Histocytochemistry; Humans; Intestinal Mucosa; Intestine, Small; Jejunum; Leucyl Aminopeptidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Carbonic Anhydrases; Diabetes Mellitus; Insulin; Islets of Langerhans; Rabbits; Rats; Tolbutamide; Zinc

Topics: Acid Phosphatase; Animals; Chelating Agents; Diabetes Mellitus; Diabetes Mellitus, Experimental; Disease Models, Animal; Histocytochemistry; Indicators and Reagents; Insulin; Islets of Langerhans; Methods; Microscopy, Fluorescence; Rabbits; Zinc

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Child; Diabetes Complications; Diabetes Mellitus; Dihydrolipoamide Dehydrogenase; Female; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Lipid Metabolism; Lung; Male; Middle Aged; Oxidoreductases; Phosphoric Monoester Hydrolases; Succinate Dehydrogenase; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Capillaries; Chronic Disease; Diabetes Complications; Diabetes Mellitus; Esterases; Female; Gingiva; Gingivitis; Histocytochemistry; Humans; Male; Oral Manifestations

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adrenal Glands; Animals; Blood Glucose; Body Weight; Diabetes Mellitus; Diabetes Mellitus, Experimental; Female; Glucosephosphate Dehydrogenase; Glycosuria; Islets of Langerhans; Kidney; L-Lactate Dehydrogenase; Langerhans Cells; Liver; Male; Microscopy; Muscles; Organ Size; Prediabetic State; Rats; Succinate Dehydrogenase; Thyroid Gland

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Alkaline Phosphatase; Angina Pectoris; Animals; Aortic Diseases; Blood Proteins; Chromatography; Chromatography, Gel; Diabetes Mellitus; Dogs; Electrocardiography; Electrophoresis; Female; Heart Failure; Humans; Hypertension; Isoenzymes; Kidney; Kidney Diseases; Liver; Liver Diseases; Lysosomes; Male; Mesenteric Arteries; Middle Aged; Mitochondria; Muscles; Myocardium; Potassium; Radionuclide Imaging; Rats; Sodium; Transaminases

Topics: Acid Phosphatase; Animals; Blood Glucose; Body Weight; Diabetes Mellitus; Female; Glucose-6-Phosphatase; Glucosephosphate Dehydrogenase; Glucosyltransferases; Glycogen; Glycosuria; Guinea Pigs; Histocytochemistry; Hydrocortisone; Islets of Langerhans; L-Lactate Dehydrogenase; Male; Time Factors

Topics: Acid Phosphatase; Acute Disease; Age Factors; Alkaline Phosphatase; Animals; Avitaminosis; Bone Neoplasms; Carcinoma; Chronic Disease; Diabetes Mellitus; Esophageal Neoplasms; Estrus; Female; Hematologic Diseases; Hemoglobinuria, Paroxysmal; Histocytochemistry; Hodgkin Disease; Humans; Infections; Leukemia; Leukocytes; Liver Diseases; Lung Neoplasms; Male; Myocardial Infarction; Neutrophils; Pregnancy; Prostatic Neoplasms; Radiation Injuries; Stress, Physiological

Topics: Acid Phosphatase; Alkaline Phosphatase; Communicable Diseases; Diabetes Mellitus; Gastrointestinal Diseases; Gastrointestinal Neoplasms; Heart Diseases; Hematologic Diseases; Humans; Kidney Diseases; Leukocytes; Liver Diseases; Lung Neoplasms; Mental Disorders; Neurologic Manifestations; Thyroid Diseases

Topics: Acid Phosphatase; Body Fluids; Diabetes Mellitus; Humans; Phosphoric Monoester Hydrolases