acid-phosphatase and Dermatitis--Contact

The functional morphology, topography and frequency of Langerhans cells (LCs), which are significant factors in the pathogenesis of contact allergic dermatitis (CAD), were studied by histoenzymatic methods (adenosine triphosphatase (ATP-ase), acid phosphatase (AF) and alpha naphtylacetate esterase (ANAE), immunohistochemical methods (indirect immunoperoxidase (IPO) with the monoclonal antibody OKT 6), and the peroxidase-antiperoxidase (PAP) method with the polyclonal S-100 antibody in skin biopsies of 24 patients with CAD, as well in skin biopsies in experimental models in guinea pigs. The results confirmed the significant role of LCs in the pathogenesis of contact allergic dermatitis.

Topics: Acid Phosphatase; Animals; Calcium-Transporting ATPases; Dermatitis, Contact; Dinitrochlorobenzene; Guinea Pigs; Humans; Immunoenzyme Techniques; Immunohistochemistry; Langerhans Cells; S100 Proteins; Skin; T-Lymphocytes

Topics: Acid Phosphatase; Animals; Benzene Derivatives; Cerebroside-Sulfatase; Dermatitis, Contact; Formaldehyde; Glucuronidase; Guinea Pigs; Lymphocytes; Lysosomes; Potassium Dichromate

Large mononuclear cells with long, actively moving cytoplasmic veils were observed in lymph coming from the skin. The enzyme histochemistry and ultrastructure of these cells suggested that they are related to epidermal Langerhans cells and interdigitating cells in the lymph node. It has been reported that Langerhans cells and interdigitating cells play a role in contact hypersensitivity by taking up antigen and presenting it to thymus-dependent lymphocytes, and it is likely that the veiled cells in the lymph are also involved. After skin-painting with 1-fluoro-2,4-dinitrobenzene (DNFB), the veiled cells in lymph coming from the site of painting became more active and were observed contacting other cells present in the lymph; many large cellular aggregates were found. Since neutrophilic leucocytes and mononuclear phagocytes were the predominating cell types in this lymph, there was no evidence for a massive recruitment of immunocompetent lymphocytes at the site of painting. Neonatally thymectomized pigs do not develop allergic reactivity to DNFB. It is of interest that the number of veiled cells and their ability to form large cellular aggregates was not affected in these animals. Therefore, it is unlikely that the defect in responsiveness can be attributed to a failure in the function of veiled cells.

Topics: Acid Phosphatase; Animals; Cytoplasm; Dermatitis, Contact; Dinitrofluorobenzene; Langerhans Cells; Lymph; Lymphocytes; Organoids; Skin; Swine; Thymectomy

Electronmicroscopical investigation on 12 cutaneous biopsies of different localisation showed that the seborrhoic dermatitis is not comparable to a psoriatic tissue-reaction. Both, the cyto-morphological feature and the demonstrated localisation of the acid phosphatase are distinctly different from that of the psoriatic epidermis. The described changes are clearly more similar to the well known ultrastructural pictures of eczemateous reactions. In spite of the "eczema-like" ultrastructural picture seborrhoic dermatitis apparently can be separated from the allergic and irritant contact-dermatitis. Nevertheless, the epidermal alterations are also similar to chronic nummular eczema. They therefore are unspecific and do not allow any conclusion regarding the etiopathogenesis or nosological classification of this skin disease.

Topics: Acid Phosphatase; Basement Membrane; Cell Nucleus; Dermatitis, Contact; Dermatitis, Seborrheic; Desmosomes; Eczema; Endoplasmic Reticulum; Histocytochemistry; Humans; Keratins; Organoids; Phagocytes; Psoriasis; Skin; Vacuoles

Topics: Acid Phosphatase; Animals; Ascitic Fluid; Autoradiography; Cell Separation; Dermatitis, Contact; Dinitrochlorobenzene; Female; Guinea Pigs; Immunity, Maternally-Acquired; Immunization; Immunization, Passive; Langerhans Cells; Lymph Nodes; Lymphocytes; Male; Microscopy, Electron; Skin; Skin Tests; Spleen; Tritium; Uridine

Topics: Acid Phosphatase; Animals; Biopsy; Chlorobenzenes; Dermatitis, Contact; Electron Transport Complex IV; Erythrocytes; Glucosephosphate Dehydrogenase; Histocytochemistry; Hydrolases; Leukocytes; Microscopy, Electron; Monocytes; Phagocytes; Skin; Succinate Dehydrogenase; Time Factors

Topics: Acid Phosphatase; Animals; Cell Migration Inhibition; Dermatitis, Contact; Electron Transport Complex IV; Glucose-6-Phosphatase; Guinea Pigs; Immunoelectrophoresis; Lymphocytes; Lysosomes; Macrophages; Microsomes; Mitochondria; Nitrobenzenes; Skin

Topics: Acid Phosphatase; Administration, Oral; Animals; Dermatitis, Contact; Female; Guinea Pigs; Male; Nitrobenzenes; Skin; Skin Absorption; Skin Tests; Vitamin A

Topics: Acid Phosphatase; Allergens; Anilides; Animals; Antigens; Cell Membrane Permeability; Cell Migration Inhibition; Culture Techniques; Dermatitis, Contact; Esterases; Guinea Pigs; Histocytochemistry; Humans; Immunity, Cellular; Lysosomes; Macrophages; Peritoneum; Photosensitivity Disorders; Salicylates; Skin Tests; Staining and Labeling

Topics: Acid Phosphatase; Adult; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Blister; Body Fluids; Cantharidin; Cathepsins; Dermatitis, Contact; Epidermolysis Bullosa; Erythema Multiforme; Glucuronidase; Humans; Insect Bites and Stings; L-Lactate Dehydrogenase; Leukocytes; Male; Pemphigus; Phenolphthaleins; Skin

Topics: Acid Phosphatase; Allergens; Animals; Dermatitis, Contact; Dinitrophenols; Female; Guinea Pigs; Histocytochemistry; Irritants; Lysosomes