acid-phosphatase and Dental-Pulp-Diseases

The present study was undertaken to determine the frequency and extent of apical root resorption associated with induced periradicular lesions in mice.. Bone and root resorption was quantified by using two- and three-dimensional micro-computed tomography (mu-CT) in the lower first molars of mice subjected to pulp exposure and infection.. mu-CT measurements showed significant apical resorption in exposed and infected teeth, resulting in an average distal root shortening of 12.7% (P <.001 vs unexposed). These findings were confirmed with three-dimensional reconstituted images that showed thinning and shortening of the distal root. Tartrate-resistant acid phosphatase clastic cells were associated with resorption lacunae on the cementum of root apices, as well as on bone at the periphery of the periradicular lesions. Brown and Brenn staining showed the presence of bacteria in dentinal tubules adjacent to resorbed cementum.. Apical root resorption is a prominent and consistent finding associated with periradicular infection in the mouse. This species represents a convenient model for studying the pathogenesis of inflammatory root resorption in vivo.

Topics: Acid Phosphatase; Animals; Biomarkers; Bone Resorption; Coloring Agents; Dental Cementum; Dental Pulp Diseases; Dental Pulp Exposure; Dentin; Disease Models, Animal; Gram-Negative Bacteria; Image Processing, Computer-Assisted; Imaging, Three-Dimensional; Isoenzymes; Male; Mandibular Diseases; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Molar; Periapical Diseases; Regression Analysis; Root Resorption; Statistics as Topic; Tartrate-Resistant Acid Phosphatase; Tomography, X-Ray Computed; Tooth Apex; Tooth Root

Attempts were made to detect and measure the activities of arylsulfatases. A&B acid phosphatase, lactate dehydrogenase, and glutamate oxaloacetate transaminase (aspartate transaminase) enzymes in human chronic lesions of endodontic origin. Thirteen periapical lesions of endodontic origin and 11 noninflamed control periapical tissues were obtained. The specimens were carried to the laboratory on liquid nitrogen and kept at -70 degrees C. Samples were thawed, homogenized, and then assayed for enzyme activities. The specific activities of arylsulfatase A (nmol/hr/mg protein) were 55.0+/-10.7 (chronic lesions) vs. 3.4+/-2.2 (controls) (p < 0.01). Arylsulfatase B specific activities (nmol/hr/mg protein) were 50.3+/-6.4 (chronic lesions) vs 91.8+/-18.4 (controls). Total acid phosphatase activities (mU/mg protein) were 45.8+/-6.6 (chronic lesions) vs. 26.8+/-3.1 (controls). Lactate dehydrogenase activities (Berger-Broida units/mg protein) of the chronic periapical lesions were significantly higher than the control group (362+/-63.2) vs. (140+/-46.0) (p < 0.05). There was no significant difference between the specific activities of aspartate transaminase in chronic lesions and the control group (68.0+/-14.5) vs. (53.0+/-10.4) mU/mg protein).

Topics: Acid Phosphatase; Aspartate Aminotransferases; Cerebroside-Sulfatase; Chronic Disease; Dental Pulp Diseases; Humans; L-Lactate Dehydrogenase; N-Acetylgalactosamine-4-Sulfatase; Periapical Diseases; Periapical Tissue; Spectrophotometry; Statistics as Topic

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Child; Dental Caries; Dental Pulp; Dental Pulp Diseases; Humans; Hydrogen-Ion Concentration; Phosphoric Monoester Hydrolases