acid-phosphatase and Demyelinating-Diseases

We studied the distribution and cytochemical characteristics of laminated bodies (LBs) in the peripheral nervous system of normal and lead-intoxicated rats. In normal rats, LBs were exclusively present in myelin-forming Schwann cells (SCs). Nerves from lead-intoxicated animals showed extensive demyelination and remyelination. In these nerves we found an increase of LBs in the SC cytoplasm, and also within phagocytes involved in myelin removal, but not in remyelinating SCs. Cytochemical studies revealed that LBs were positive for acid phosphatase, thus demonstrating the lysosomal nature of such inclusions. Taken together, these data suggest that LBs are autophagolysosomes derived from myelin catabolism, which may be enhanced in the lead-induced demyelinating neuropathy of the rat. The possible mechanisms underlying this phenomenon and their pathological relevance are discussed.

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; Ganglia; Histocytochemistry; Inclusion Bodies; Lead; Lysosomes; Microscopy, Electron; Myelin Sheath; Peroneal Nerve; Phagocytes; Rats; Rats, Inbred Strains; Schwann Cells

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; Female; Immune Sera; Macrophages; Myelin Sheath; Neuritis; Phagocytosis; Rabbits

In a combined histological, biochemical and histochemical study of the macroscopically normal white matter in multipe sclerosis 72% of samples were histologically abnormal. The significance of this fact in the interpretation of previous biochemical studies and in the design of future studies is discussed. The present study showed a significant elevation of the lysosomal enzyme beta-glucosaminidase in the microscopically normal white matter in MS as compared with controls. Studies on lysosomes separated from microscopically normal or mild to moderately gliosed white matter in multiple sclerosis showed an increase in lysosomal fragility. Histochemical study of the microscopically normal white matter in multiple sclerosis revealed an increase in the number of acid phosphate-containing cells as compared with normal and neurological control material. The significance of these findings is discussed and it is suggested that irrespective of the primary or secondary nature of these abnormalities, the white matter may be rendered more susceptible to the pathogenetic process in this disease.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Brain; Demyelinating Diseases; Female; Gliosis; Humans; Lipofuscin; Lysosomes; Male; Middle Aged; Multiple Sclerosis

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; Disease Models, Animal; Macrophages; Peptide Hydrolases; Peripheral Nerves; Polyradiculoneuropathy; Rabbits; Spinal Nerve Roots

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; Disease Models, Animal; DNA; Encephalomyelitis, Autoimmune, Experimental; Glucosephosphate Dehydrogenase; Haplorhini; Humans; Hydrolases; L-Lactate Dehydrogenase; Mice; Multiple Sclerosis; Wallerian Degeneration

The fine structural characteristics of storage products in peripheral nerve, kidney and cerebral white matter, from a case of adult metachromatic leukodystrophy are described. There were pronounced differences from the fine structural aspects in late infantile cases. A large proportion of the inclusions did not exhibit a unit membrane. An hypothesis is proposed to clarify the delayed manifestation of this type of metachromatic leukodystrophy until childhood.

Topics: Acid Phosphatase; Adult; Brain; Demyelinating Diseases; Humans; Inclusion Bodies; Kidney Tubules; Kidney Tubules, Distal; Leukodystrophy, Metachromatic; Lysosomes; Macrophages; Male; Neurons; Peripheral Nerves; Schwann Cells; Sulfoglycosphingolipids

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Cats; Cerebrospinal Fluid; Demyelinating Diseases; Disease Models, Animal; Lysosomes; Microscopy, Electron; Neuroglia; Time Factors

Topics: Acid Phosphatase; Biopsy; Collagen; Demyelinating Diseases; Histocytochemistry; Humans; Hypertrophy; L-Lactate Dehydrogenase; Leprosy; Macrophages; Microscopy, Electron; Mycobacterium leprae; Myelin Sheath; Nerve Degeneration; Peripheral Nerves; Peripheral Nervous System Diseases; Schwann Cells; Sural Nerve

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; DNA; Galactosidases; Glucosephosphate Dehydrogenase; Glucuronidase; Glycerolphosphate Dehydrogenase; Hydrolases; L-Lactate Dehydrogenase; Lipid Metabolism; Lysosomes; Mice; Peptide Hydrolases; Spinal Cord

Topics: Acetylcholinesterase; Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Brain; Cholinesterases; Demyelinating Diseases; Esterases; Glycerolphosphate Dehydrogenase; Histocytochemistry; Hydrogen Cyanide; Hydroxybutyrate Dehydrogenase; NADH, NADPH Oxidoreductases; Neuroglia; Poisoning; Rats; Succinate Dehydrogenase; Tetrazolium Salts; Thiamine; Time Factors; Transferases

Topics: Acid Phosphatase; Animals; Complement System Proteins; Culture Techniques; Demyelinating Diseases; Encephalomyelitis, Autoimmune, Experimental; Galactosidases; Glucosephosphate Dehydrogenase; Glucuronidase; Isocitrate Dehydrogenase; Mice; Myelin Sheath; Nucleotides; Phosphoric Monoester Hydrolases; Spinal Cord; Sulfatases; Sulfoglycosphingolipids; Tissue Extracts

Topics: Acetylcholinesterase; Acid Phosphatase; Adenosine Triphosphatases; Adult; Alkaline Phosphatase; Autopsy; Brain; Cholinesterases; Demyelinating Diseases; Esterases; Humans; Multiple Sclerosis; Neuroglia; Phosphoric Monoester Hydrolases; Pyrophosphatases; Sulfatases; Thiamine

Topics: Acid Phosphatase; Animals; Cell Separation; Demyelinating Diseases; Electrophoresis, Polyacrylamide Gel; Encephalomyelitis, Autoimmune, Experimental; Female; Freund's Adjuvant; Guinea Pigs; Hexosaminidases; Leukocyte Count; Lymphocytes; Lysosomes; Male; Myelin Basic Protein; Nitrophenols; Phosphates; Ultrasonics

Topics: Acid Phosphatase; Brain Chemistry; Cerebrosides; Cholesterol; Demyelinating Diseases; Humans; Lysosomes; Multiple Sclerosis; Myelin Sheath; Nerve Tissue Proteins; Nucleotides; Phospholipids; Phosphoric Monoester Hydrolases; Subacute Sclerosing Panencephalitis

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Animals, Newborn; Brain Neoplasms; Demyelinating Diseases; Encephalomyelitis, Autoimmune, Experimental; Esterases; Fetus; Guinea Pigs; Humans; Mice; Multiple Sclerosis; Myelin Sheath; Neuroglia; Oligodendroglioma; Phosphoric Monoester Hydrolases; Rabbits

Topics: Acid Phosphatase; Adenosine Triphosphatases; Aerosols; Animals; Brain Diseases; Corpus Callosum; Cyanides; Demyelinating Diseases; Histocytochemistry; Necrosis; Neuroglia; Oxidoreductases; Phosphoric Monoester Hydrolases; Rats; Thiamine Pyrophosphate

Topics: Acid Phosphatase; Animals; Cytoplasm; Demyelinating Diseases; Diphtheria Toxin; Female; Histocytochemistry; Lysosomes; Male; Microscopy, Electron; Myelin Sheath; NADH, NADPH Oxidoreductases; Oxidoreductases; Peripheral Nerves; Ranvier's Nodes; Rats; Schwann Cells; Sciatic Nerve; Tetrazolium Salts

Topics: Acid Phosphatase; Animals; Brain Chemistry; Cattle; Demyelinating Diseases; Encephalomyelitis, Autoimmune, Experimental; Female; Guinea Pigs; Injections, Subcutaneous; Male; Methods; Peptide Hydrolases; Proteins; Spinal Cord

Topics: Acid Phosphatase; Age Factors; Animals; Brain; Cerebral Cortex; Corpus Callosum; Demyelinating Diseases; Diffuse Cerebral Sclerosis of Schilder; Disease Models, Animal; Female; Male; Mice; Mice, Inbred Strains; Microscopy, Electron; Myelin Sheath; Neuroglia; Peripheral Nerves

Topics: Acid Phosphatase; Aged; Axons; Bronchopneumonia; Cholesterol; Demyelinating Diseases; Esterases; Humans; Male; Myelin Sheath; Peptide Hydrolases; Peripheral Nerves; Phospholipids; Polyradiculopathy; Spinal Nerve Roots; Spinal Nerves

Topics: Acid Phosphatase; Aging; Alkaline Phosphatase; Animals; Central Nervous System; Central Nervous System Diseases; Cerebrosides; Cholesterol; Demyelinating Diseases; Disease Models, Animal; Female; Genes, Recessive; Genotype; Lipids; Male; Metabolism, Inborn Errors; Mice; Mutation; Phosphoric Monoester Hydrolases; Thyroid Function Tests; Time Factors

Topics: Acid Phosphatase; Adult; Biopsy; Cytoplasmic Granules; Demyelinating Diseases; Endoplasmic Reticulum; Female; Golgi Apparatus; Histocytochemistry; Humans; Lysosomes; Male; Microscopy, Electron; Peripheral Nerves; Peripheral Nervous System Diseases; Schwann Cells

Topics: Acid Phosphatase; Animals; Cell Count; Demyelinating Diseases; Histocytochemistry; Inclusion Bodies; Lipids; Male; Microscopy, Electron; Nerve Degeneration; Neuroglia; Optic Nerve; Phagocytosis; Rats

Topics: Acid Phosphatase; Animals; Axons; Cell Membrane; Demyelinating Diseases; Female; Ganglia, Spinal; Goats; Histocytochemistry; Male; Myelin Sheath; Nerve Degeneration; Peripheral Nervous System Diseases; Plant Poisoning; Schwann Cells; Sciatic Nerve; Spinal Cord; Time Factors

Topics: Acid Phosphatase; Age Factors; Animals; Axons; Cholinesterases; Copper; Deficiency Diseases; Demyelinating Diseases; Electron Transport Complex IV; Histocytochemistry; Lysosomes; Muscles; Neuromuscular Junction; Sciatic Nerve; Sheep; Sheep Diseases; Species Specificity; Spinal Cord; Swayback

Topics: Acid Phosphatase; Animals; Brain; Demyelinating Diseases; Encephalomyelitis, Autoimmune, Experimental; Guinea Pigs; Lysosomes; Myelin Sheath; Peptide Hydrolases

Topics: Acid Phosphatase; Biopsy; Cytoplasm; Demyelinating Diseases; Diffuse Cerebral Sclerosis of Schilder; Freezing; Humans; Infant; Microscopy, Electron; Peripheral Nerves; Schwann Cells; Transferases

Topics: Acid Phosphatase; Atrophy; Brain; Cerebellum; Cerebral Cortex; Demyelinating Diseases; Diffuse Cerebral Sclerosis of Schilder; Electron Transport Complex IV; Glucuronidase; Histocytochemistry; Humans; Infant; Male; Nerve Tissue Proteins; Nitrogen; Peripheral Nervous System Diseases

Topics: Acid Phosphatase; Animals; Demyelinating Diseases; Diphtheria; Histocytochemistry; Lysosomes; Macrophages; Nerve Degeneration; Peripheral Nervous System Diseases; Poultry; Sciatic Nerve

Topics: Acid Phosphatase; Brain; Cerebrosides; Chemistry Techniques, Analytical; Demyelinating Diseases; Diffuse Cerebral Sclerosis of Schilder; Diseases in Twins; Galactosidases; Histocytochemistry; Humans; Hydro-Lyases; In Vitro Techniques; Kidney; Lipid Metabolism, Inborn Errors; Lipidoses; Liver; Microscopy, Electron; Mucopolysaccharidoses; Sulfatases