acid-phosphatase and Colitis

The aim of this study was to investigate the cellular and molecular expression of tartrate resistant acid phosphatase (TRAP) as a marker of activated macrophages in macrophage dependent dextran sulphate sodium (DSS)-induced colitis in rats. In normal colon, TRAP+/CX(3)CR(1)+ macrophages were located in the upper part of the lamina propria. In the early stage (day 1-3) of acute colitis prior to histopathological changes, induction of the cytokines TNFalpha, IL-12 and IFN gamma occurred concomitant with increased mRNA and enzyme activity of TRAP along with a slight increase of TRAP immunolabelling in macrophages of the upper lamina propria, suggesting induction of TRAP in resident macrophages. Among these cytokines, TNFalpha up-regulated TRAP expression in the RAW 264.7 monocyte/macrophage cell line. In a later phase (day 7) with fulminant colitis, a massive infiltration of macrophages including recruited TRAP+/CCR2+ cells was observed also in the lower part of the lamina propria as well as in the submuscular layer. Additionally, differentiated cellular expression of pro- and mature TRAP also suggest that mucosal macrophages in the lower part of lamina propria bordering the sub-mucosa provide a source of replenishment of macrophages situated in the upper lamina propria. In conclusion, induction of TRAP provides an early sign of macrophage responsiveness in DSS induced colitis.

Topics: Acid Phosphatase; Animals; Biomarkers; Colitis; Colon; Cytokines; Dextran Sulfate; Isoenzymes; Macrophage Activation; Rats; RNA, Messenger; Tartrate-Resistant Acid Phosphatase; Transcriptional Activation

Eicosanoids and platelet-activating factor (PAF) production increases in experimental colitis. Both eicosanoids and PAF seem to arise from similar membrane phospholipids. To support both these suggestions we have investigated whether a fat-free diet, which should alter production of eicosanoids and PAF, affects experimental colitis. Essential fatty acid deficient (EFAD) rats were obtained by putting 4-week-old animals on a fat-free diet for 3 months. Experimental colitis was induced by a single intracolonic administration of 2 ml of 4% acetic acid. One to seven days later the animals were sacrificed and the colon removed to assess macroscopically and histologically intestinal damage. Eicosanoids and PAF levels were also measured in the mucosa scrapings by specific radioimmunoassay. The injury to the colon was more evident in control rats compared with EFAD rats. Besides colonic tissue of control rats showed a highly significant increase of PGE2, LTB4 and PAF, compared with levels in EFAD rats. Our results indicate that fat-free diet reduces tissue damage, and at the same time PGE2, LTB4 and PAF colonic content.

Topics: Acetates; Acetic Acid; Acid Phosphatase; Animals; Colitis; Dietary Fats; Dinoprostone; Fatty Acids; Leukotriene B4; Male; Platelet Activating Factor; Rats; Rats, Wistar

The purpose of the studies was to determine the activity of enzymes in the serum and pig organs with colibacteriosis in the form of oedema and stomach-intestines disease playing a diagnostic role in the determination of the organ damage degree. Studies were carried out on 29 pigs. The activity of LAP, GPT, GOT, AM, FDPA, Lp, AP and AcP was determined in the serum liver, empty intestines and kidneys of the diseased and control pigs. It resulted from the experiments that in the serum of all the diseased animals the activity of FDPA and GOT was considerably increased and that of AP decreased. So, activity determination does not allow to differentiate both forms of colibacteriosis. AM activity increases only in the serum of oedema pigs. In colibacteriosis of pigs the determination of FDPA activity is the most sensitive enzymatic test. In both diseases the activity of this enzyme increases considerable in the serum and all the studied organs. The activity of the studied enzymes shows that in both forms of collibacteriosis of pigs liver, intestines and kidneys are damaged.

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; alpha-Amylases; Animals; Colitis; Colon; Escherichia coli Infections; Fructose-Bisphosphate Aldolase; Kidney; Leucyl Aminopeptidase; Lipase; Liver; Swine; Swine Diseases

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Colitis; Esterases; Female; Humans; Intestinal Diseases; Isoenzymes; L-Lactate Dehydrogenase; Malate Dehydrogenase; Male; Middle Aged

Topics: Acid Phosphatase; Adenocarcinoma; Aged; Anorexia Nervosa; Barium Sulfate; Biopsy, Needle; Blood Urea Nitrogen; Castration; Colitis; Diarrhea; Heart Failure; Humans; Intestinal Mucosa; Lumbar Vertebrae; Male; Prostatic Neoplasms; Radiotherapy Dosage; Radiotherapy, High-Energy; Spinal Diseases; Uric Acid

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Blood Glucose; Cholesterol; Chronic Disease; Colitis; Female; Humans; Liver; Male; Mineral Waters; Prothrombin Time; Therapeutic Irrigation