acid-phosphatase and Colitis--Ulcerative

To study the therapeutic effects and mechanism of Jiechangning (JCN) decoction on carrageenan induced experimental ulcerative colitis (UC).. After sensitizing guinea pigs with carrageenan, we established UC animal models by free drinking water containing 2% acid degraded carrageenan (ADC). JCN decoction was orally administered once a day for 2 weeks after carrageenan treatment. Salicylazosulfapyridine (SASP) and normal saline were given to the other two groups as control. The levels of colon lipid peroxide (LPO), acid phosphatase (ACP) activity and tumor necrosis factor-alpha (TNF-alpha) were measured; colitis activity score (CAS) was carried out for assessment of the degree of tissue inflammation and injury; the colonic pathological changes were examined simultaneously with hematoxylin and eosin (HE) and toluidine blue staining used to evaluate the therapeutic effects of JCN decoction and SASP.. Experimental colitis models resembling human UC were successfully induced. The levels of tissue LPO, ACP activity and the content of tissue TNF-alpha were markedly increased in the model group as compared with the normal control group (P < 0.01) and were positively correlated with CAS. JCN decoction could reverse these changes like SASP. HE staining showed that JCN decoction and SASP could reduce CAS and the degree of tissue injury, toluidine blue staining revealed that mucosa and submucosa red metachromasia pellets in JCN group and SASP group were markedly fewer than those in the model group.. JCN decoction is effective in treating experimental UC, which provides theoretical basis for its clinical application.

Topics: Acid Phosphatase; Animals; Carrageenan; Colitis, Ulcerative; Colon; Gastrointestinal Agents; Guinea Pigs; Lipid Peroxides; Male; Medicine, Chinese Traditional; Plant Preparations; Sulfasalazine; Tumor Necrosis Factor-alpha

This study was carried out to evaluate the clinical validity and usefulness of serum tartrate-resistant fluoride-sensitive acid phosphatase (TrFsACP) activity using 2,6-dichloro-4-acetylphenyl phosphate as substrate at pH 6.2 in metabolic bone diseases. The mean Z-scores of TrFsACP activity in patients on hemodialysis were higher than in healthy subjects (male: 2.04+/-1.98, n = 49, P < .05; female: 1.49+/-2.43, n = 39, P < .05) and increased with duration of hemodialysis (r = .516, P < .01). Bone alkaline phosphatase also was found to be significantly higher in hemodialysis patients (male: 0.93+/-1.49, P < .05; female: 1.66+/-2.42, P < .05) compared with normal subjects: but had lower correlation with duration of hemodialysis than TrFsACP (r = .277, P < .05). Ulcerative colitis (1.37+/-2.21, n = 15) in males showed a significantly higher Z-score of TrFsACP compared with control subjects (P < .05). The relationship of TrFsACP activity and ultrasound findings (stiffness; speed of sound [SOS]; broadband ultra sound attenuation [BUA]) in healthy women aged 30-75 years (n = 95) were inversely and significantly correlated with stiffness (r = -.465, P < .01 ), SOS (r = -.484, P < .01), and BUA (r = -.366, P < .01), but were age dependent. TrFsACP activity significantly correlated with stiffness (r = -.521, P < .05) and SOS (r = -.527, P < .05) only in the age group of 46-55 years. BUA (r = -.313, P > .05) did not correlate significantly in any subject in the present study. We conclude that serum TrFsACP activity is useful in the diagnosis and monitoring of bone turnover.

Topics: Acid Phosphatase; Adult; Aged; Arthritis, Rheumatoid; Biomarkers; Bone and Bones; Bone Remodeling; Case-Control Studies; Colitis, Ulcerative; Female; Fluorides; Humans; Male; Middle Aged; Osteogenesis Imperfecta; Osteoporosis; Predictive Value of Tests; Reference Values; Renal Dialysis; Tartrates; Ultrasonography

Inflammatory bowel diseases (IBD) as Crohn's disease (CD) and ulcerative colitis (UC) are believed to have a genetic basis. Additional factors are supposed to promote the development of IBD. However, apart from a few reports of HLA associations which await confirmation by other groups strong associations to (a) particular genetic marker(s) are still lacking. We here report on previously unobserved associations of CD to MNSS and UC to the immunoglobulin heavy chain allotype Gm 1,-2,10. We suggest that these factors play a role in a wider spectrum of genetic markers for the development of IBD.

Topics: Acid Phosphatase; Adenosine Deaminase; Adenylate Kinase; Alanine Transaminase; Blood Group Antigens; Carboxylesterase; Carboxylic Ester Hydrolases; Colitis, Ulcerative; Crohn Disease; Erythrocytes; Genetic Markers; Humans; Immunoglobulin Gm Allotypes; Phenotype; Phosphoglucomutase; Phosphogluconate Dehydrogenase; Polymorphism, Genetic

The mononuclear cell system in the human gut wall of patients with Crohn's disease (CD), ulcerative colitis (UC) and normal controls was studied, with special reference to the so called antigen presenting veiled cells. These cells have already extensively been studied in the skin and are known as Langerhans' cells in the epidermis and dermis, veiled cells in the skin lymph and interdigitating cells in lymph nodes. Recently they were also found in gut associated lymphoid tissue, i.e. Peyer's patches of the rat. Here we describe the presence of similar cells in chronic idiopathic inflammatory bowel disease (CIBD). They resemble veiled cells in moving pattern, strong Ia positivity, no or only weak acid phosphatase activity, and ultrastructure. However, many of the described cells combine these characteristics with those of phagocytic macrophages. In the gut wall of controls veiled cells were virtually absent and phagocytic macrophages were almost exclusively recognized. These findings suggest that more intensive antigen handling takes place in the gut wall of CIBD patients than in normal gut. Clear cut associations with sex, age, duration or activity of disease were not observed in this limited study, and the exact significance of the presence of such cells needs further clarification.

Topics: Acid Phosphatase; Adolescent; Adult; Colitis, Ulcerative; Colon; Crohn Disease; Female; Histocompatibility Antigens Class II; Humans; Ileum; Macrophages; Male; Microscopy, Electron; Middle Aged; Phagocytosis

Topics: Acid Phosphatase; Adult; Colitis, Ulcerative; Female; Humans; Male; Middle Aged

Topics: Acid Phosphatase; Adolescent; Adult; Cholinesterases; Colitis, Ulcerative; Female; Granulocytes; Humans; Leukocytes; Lymphocytes; Lysosomes; Male; Phagocytosis

Homogenates of gut and mesenteric lymph node tissue from patients with Crohn's disease, ulcerative colitis, and control patients were tested for cytopathic effect (CPE) production in a series of cell lines including rabbit ileal and W138 cells. A CPE was produced in W138 cells by tissue homogenates from patients with Crohn's disease, ulcerative colitis, and control patients. Evidence was obtained to suggest that this effect, although superficially similar to a viral CPE, was due to toxic protein constituents of inflamed tissue homogenate.

Topics: Acid Phosphatase; Cell Line; Colitis, Ulcerative; Crohn Disease; Cytopathogenic Effect, Viral; Humans; Intestines; Lymph Nodes; Viruses

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Biopsy; Chronic Disease; Colectomy; Colitis, Ulcerative; Dihydrolipoamide Dehydrogenase; Drug Therapy, Combination; Galactosidases; Glucosephosphate Dehydrogenase; Glucosidases; Histocytochemistry; Humans; Intestinal Mucosa; Jejunum; Prednisone; Succinates; Sulfasalazine

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Binding Sites; Biopsy; Colitis, Ulcerative; Colon; Feces; Glucuronidase; Humans; Intestinal Mucosa; Lymphocytes; Lysosomes; Middle Aged; Phenylalanine; Sulfatases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Biliary Tract Diseases; Cholecystectomy; Cholelithiasis; Chronic Disease; Colitis, Ulcerative; Diabetes Mellitus; Duodenum; Gastrectomy; Gastritis; Histocytochemistry; Humans; Intestinal Mucosa; Intestine, Small; Jejunum; Leucyl Aminopeptidase; Succinate Dehydrogenase

An electron microscopic study of Crohn's disease of the colon is presented. The positive findings that supplement those obtained by light microscopy are: a prominent nucleolus is more common in the lymphocytes of Crohn's disease than in normal lymphocytes or those found in cases of ulcerative colitis; lymphocytes are often observed close to macrophages and epithelioid cells; the epithelioid cells are metabolically active, and have vesicles which contain acid phosphatase but little visible debris; intramural bacteria were identified in six of the 11 specimens of colon with intact epithelia and minimal inflammatory changes. The possible significance of these findings for the pathogenesis of the disease is discussed.

Topics: Acid Phosphatase; Adult; Aged; Cell Nucleolus; Colitis, Ulcerative; Colon; Crohn Disease; Epithelium; Female; Humans; Lymphocytes; Macrophages; Microscopy, Electron; Middle Aged

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Biopsy; Colitis, Ulcerative; Enzymes; Esterases; Histocytochemistry; Humans; Hydrolases; Intestinal Mucosa; Oxidoreductases

Topics: Acid Phosphatase; Alkaline Phosphatase; Biopsy; Colitis, Ulcerative; Colon, Sigmoid; Esterases; Histocytochemistry; Humans; Intestinal Mucosa; Rectum

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Antibody Formation; Autoantibodies; Colitis, Ulcerative; Colon; Escherichia coli; Esterases; Fluorescent Antibody Technique; Glucosephosphate Dehydrogenase; Humans; Immunization; Intestinal Mucosa; Rabbits; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Autopsy; Biopsy; Colitis, Ulcerative; Colon; Esterases; Fluorouracil; Histocytochemistry; Humans; Intestinal Mucosa; Intestine, Small; Jejunum; Mice; NADP; Oxidoreductases