acid-phosphatase and Classical-Swine-Fever

The activities of proteinases active at neutral pH and of acid phosphatase increases after freezing and defrosting and hypotonic shock in porcine leukocytes in vitro infected with the classical hog cholera virus in comparison with intact cells. The enzymes in the cells from animals both immune and nonimmune to hog cholera were activated upon infection with both virulent and vaccine strains of the virus. No proteinase activity was detected in the culture medium in which infected porcine leukocytes were cultured. Possible pathological reactions of a cell to viral infection are discussed.

Topics: Acid Phosphatase; Animals; Classical Swine Fever; Endopeptidases; Enzyme Activation; Freezing; Hydrogen-Ion Concentration; Leukocytes; Osmolar Concentration; Swine

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Classical Swine Fever; Leukocytes; Male; Peroxidases; Swine; Vaccination

Nonvaccinated pigs were infected with a pathogenic virus of swine fever in order to follow up the changes with some enzymes in their blood serum. It was found that there is a dependable rise in the values of the blood serum GOT (2.6.1. 1.) - From about 38 to approximately 108 mU per cu. cm; GPT (2. 6; 1.2.;-From about 12 up to 66 mU/cu. cm; LAP (3. 4. 1. 1)-from about 13 to 27 mU/cu. cm; and ALD (4. 1; 2. 7.) - from about 6 up to 19 mU/cu. cm. These changes were noticed as early as the 48th hour of infection, prior to the manifestation of the clinical symptoms of swine fever. They remained stable up to the end of the infection process. The activity of LDH (1. 1. 2. 7.) and amylase (3. 2. 1.1 .) was said to rise (not dependable statistically), and that of cholinesterase (3. 1 1. 8) - to drop gradually with the development of the infection process. The two phosphomonoesterases (3. 1. 3. 1 and 3. 1. 3. 2.) showed no essential changes. The changes taking place in the activity of these enzymes did not depend on the extent of the hemorrhagic diathesis characteristic of swine fever. So, this test, which has been unknown so far, is referred to as a characteristic paraclinical symptom of swine fever.

Topics: Acid Phosphatase; Acute Disease; Alanine Transaminase; Alkaline Phosphatase; Amylases; Animals; Aspartate Aminotransferases; Bilirubin; Cholinesterases; Classical Swine Fever; Fructose-Bisphosphate Aldolase; L-Lactate Dehydrogenase; Swine