acid-phosphatase and Cicatrix

Activities of acid and alkaline phosphatases, cathepsin B, leucine aminopeptidase, N-acetyl-beta-D-hexosaminidases, beta-D-galactosidase and beta-D-glucuronidase were studied in leukocytes of two groups of patients with normal and pathological healing of wounds after surgical treatment of scar stenosis of larynx. Analysis of the enzymatic activity, calculated by means of Student's test using the data of a single estimation of the activity in leukocytes before surgical treatment, did not exhibit any distinct differences in these two groups of patients. The study of interrelationship between the activities of individual enzymes by means of principles of regression analysis enabled to show that the enzymes studied were in the positive regressive relation. Pattern of enzymatic activity in leukocytes of patients with pathological wound healing was distinctly different from that of patients with normal healing in the shape of regression and, especially, in relaxation of the interrelations between phosphatases and other enzymes. The principle of regression enabled to evaluate the prognosis of postoperative development of wound healing using the preliminary estimation of enzymatic activity in leukocytes of patients with scar stenosis of larynx.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; beta-Galactosidase; beta-N-Acetylhexosaminidases; Cathepsin D; Cicatrix; Constriction, Pathologic; Hexosaminidases; Humans; Laryngeal Diseases; Leukocytes; Lysosomes; Middle Aged; Regression Analysis

The healing of excisional wounds in rats, which were treated with zinc tape, gauze sponge or porcine skin, was studied. Wound closure was completed earlier in zinc-tape-treated wounds than in wounds treated with a gauze sponge or porcine skin. Wound contraction was more pronounced in gauze- and porcine skin-treated wounds than in zinc-tape-treated wounds. More foreign body giant cells were seen 14 days after operation in gauze-treated contra zinc tape-treated wounds, whereas hydroxyproline concentration was higher in tape-treated wounds. Histochemically the acid phosphatase activity was pronounced in macrophages, foreign body giant cells and fibroblasts. The alkaline phosphatase activity was pronounced in granulocytes and fibroblasts. Quantitatively a reduction in alkaline phosphatase activity was seen in gauze-treated wounds from 7 to 14 days. A decrease was seen in albumin concentration in gauze- as well as zinc-tape-treated animals which was most pronounced after 7 days in the gauze-treated animals.

Topics: Acid Phosphatase; Administration, Topical; Alkaline Phosphatase; Animals; Cicatrix; Histocytochemistry; Hydroxyproline; Male; Rats; Serum Albumin; Skin; Skin Transplantation; Time Factors; Wound Healing; Zinc

Biopsies from non-hypertrophic and hypertrophic scars and from normal skin have been studied histochemically for activities of nicotanamide adenine dinucleotide diaphorase, lactate dehydrogenase, acid phosphatase, beta-D glucuronidase and alkaline phosphatase. The activities of all enzymes studied except alkaline phosphatase were found to be increased in hypertrophic scars as compared with non-hypertrophic scars and normal skin.

Topics: Acid Phosphatase; Alkaline Phosphatase; Cicatrix; Glucuronidase; Humans; Hypertrophy; Keloid; L-Lactate Dehydrogenase; NADPH Dehydrogenase; Skin

Topics: Acid Phosphatase; Alkaline Phosphatase; Cicatrix; Glucuronidase; Histocytochemistry; Humans; Hypertrophy; Keloid; NAD; Skin; Wound Healing

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Blood Vessels; Cicatrix; Dogs; Fibroblasts; Histocytochemistry; Inflammation; Peritoneum; Polyglycolic Acid; Postoperative Complications; Staining and Labeling; Sutures; Time Factors; Tissue Adhesions; Vascular Surgical Procedures; Vena Cava, Inferior

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Aminopeptidases; Animals; Bile; Cell Division; Cell Movement; Cicatrix; Common Bile Duct; Epithelial Cells; Epithelium; Esterases; Female; Glucuronidase; Histocytochemistry; Male; Mucins; Rats; Succinate Dehydrogenase; Wound Healing

Topics: Acid Phosphatase; Biopsy; Cicatrix; Esterases; Histocytochemistry; Humans; Lipids; Psoriasis; Skin