acid-phosphatase and Cholestasis

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aged; Alkaline Phosphatase; Animals; Bile Ducts; Bile Ducts, Intrahepatic; Bilirubin; Cholestasis; Cholesterol; Female; Histocytochemistry; Humans; Infant; Liver; Nucleotidases; Rats

Acid phosphatase, alkaline phosphatase and 5'-nucleotidase activities were analyzed cytophotometrically in cryostat sections of rat liver up to 8 weeks after ligation and transsection of the common bile duct. Ligation resulted in cholestasis and induced alterations in both localization and activity of the enzyme investigated. The cellular distribution but not the activity of acid phosphatase changed in liver parenchyma. In control liver, the final reaction product was localized as discrete granules in the bile canalicular region of hepatocytes. The final reaction product was precipitated more diffusely within the cytoplasm after induction of cholestasis, most probably due to increased fragility of lysosomal membranes. In control liver, alkaline phosphatase activity was low and localized in the bile canalicular plasma membranes only. The total parenchymal activity increased threefold after the induction of cholestasis and is considered to be a compensatory mechanism in order to enhance the excretion of bile salts from hepatocytes. 5'-Nucleotidase was present at the bile canalicular and sinusoidal surfaces of plasma membranes of hepatocytes in control liver; total activity in pericentral areas was significantly higher than in periportal areas. Induction of cholestasis resulted in higher total activity and redistribution of the activity over all three surfaces of the plasma membranes, whereas heterogeneity over the different zones of the acinus disappeared. The appearance of the enzyme at lateral plasma membranes is suggested to be related to the formation of new sites for bile salt transport out of the hepatocytes. With respect to all three enzymes studied, alterations of liver parenchymal cells due to a disturbed bile transport were already established during the first week of cholestasis.

Topics: 5'-Nucleotidase; Acid Phosphatase; Alkaline Phosphatase; Animals; Cholestasis; Common Bile Duct; Cytophotometry; Ligation; Liver; Male; Rats; Rats, Inbred Strains; Time Factors

Deposits of bile in the cytoplasm of hepatocytes from six patients with cholestasis were studied by electron microscopy and ultrastructural cytochemistry. The deposits were identified in vacuoles of varying size and complexity. The demonstration of acid phosphatase activity in these vacuoles suggests that lysosomes participate in the elimination of bile deposits retained in hepatocytes.

Topics: Acid Phosphatase; Bile Pigments; Biopsy; Cholestasis; Humans; Liver; Lysosomes; Microscopy, Electron

In the cholestatic condition of many different aetiologies a common pathologic picture is found. It is characterized by the presence of pigmented "plugs" and cytoplasmatic "masses" in the centrolobular region. Dubin has tried to interpret this on purely hydrostatic ground but this is not a very likely explanation. We tried to comprove this hypothesis studying the interference of a previous, or concomitant, centrolobular alteration in the distribution, morphology and intensity of cholestatic lesions: terminal choledochus ligatures were performed in animals intoxicated with carbon tetrachloride, a substance that damages predominantly the centrolobular region. In 6 dogs, terminal choledochus ligature was associated with carbon tetrachloride intoxication. A histological examination of the liver showed that the degree of centrolobular cholestasis was in the inverse ration of hepatocytic damage intensity. This fact does not conform with Dubin's hypothesis.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Cholestasis; Dogs; Liver; Male

Under study was the effect of hyperbaric oxygenation in 46 patients on the course of bile tract diseases, complicated with hepatic insufficiency. Hyperbaric oxygenation was found to render a positive effect on the course of hepatic insufficiency.

Topics: Acid Phosphatase; Acute Disease; Aged; Alkaline Phosphatase; Cholecystitis; Cholestasis; Chronic Disease; Electron Transport Complex IV; Female; Glycogen; Humans; Hyperbaric Oxygenation; Liver; Male; Middle Aged; Oxygen Consumption; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bile Duct Neoplasms; Biopsy; Cholestasis; Glucose-6-Phosphatase; Histocytochemistry; Humans; Hydroxybutyrate Dehydrogenase; Liver

Topics: Acid Phosphatase; Cholestasis; Clinical Enzyme Tests; Humans; Methods

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Biopsy; Cholestasis; Dogs; Esterases; gamma-Glutamyltransferase; Glucose-6-Phosphatase; Glucuronidase; Histocytochemistry; Humans; Kupffer Cells; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Liver; Liver Cirrhosis, Biliary; Microscopy, Electron; Monoamine Oxidase; Oxidoreductases; Phosphoric Monoester Hydrolases; Rats; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Bile Ducts; Bilirubin; Cholestasis; Cholesterol; Dogs; Hepatic Veins; Liver; Liver Cirrhosis, Biliary; Lymphatic Diseases; Lymphatic System; Nucleotidases; Vena Cava, Superior

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Cholestasis; Cholinesterases; Clinical Enzyme Tests; Diagnosis, Differential; Ethionine; Fatty Liver; Fructose-Bisphosphate Aldolase; Fructosephosphates; Necrosis; Phenobarbital; Proadifen; Rats; Thioacetamide

Topics: Acid Phosphatase; Acute Disease; Adenosine Triphosphatases; Adult; Aged; Alcoholism; Alkaline Phosphatase; Biopsy; Chemical and Drug Induced Liver Injury; Cholestasis; Chronic Disease; Enzymes; Fatty Liver; Female; Hepatitis A; Histocytochemistry; Humans; Hydroxybutyrate Dehydrogenase; Liver; Liver Cirrhosis; Liver Diseases; Male; Middle Aged

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Bile Acids and Salts; Bilirubin; Biopsy; Cholestasis; Cytoplasm; Cytoplasmic Granules; Cytosol; Glucuronidase; Inclusion Bodies; Kidney; Kidney Tubules; Ligation; Lysosomes; Male; Pigments, Biological; Rats; Rats, Inbred Strains; Time Factors

Topics: Acid Phosphatase; Acyltransferases; Adenosine Triphosphatases; Adult; Aged; Alkaline Phosphatase; Biopsy; Cholestasis; Common Bile Duct; Esterases; Female; Glucose-6-Phosphatase; Glucuronidase; Glutamates; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Liver; Male; Middle Aged; Monoamine Oxidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Animals; Bile Ducts; Cholestasis; Cytoplasm; Endoplasmic Reticulum; Extracellular Space; Golgi Apparatus; Histocytochemistry; Ligation; Liver; Lysosomes; Male; Microtubules; Rats; Rats, Inbred Strains

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Bile Ducts, Intrahepatic; Cholestasis; Cytosol; Histocytochemistry; Kidney Tubules; Ligation; Lysosomes; Male; Microscopy, Electron; Pinocytosis; Rats; Rats, Inbred Strains; Time Factors

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Antilymphocyte Serum; Azathioprine; Bilirubin; Biopsy; Chemical and Drug Induced Liver Injury; Cholestasis; Dogs; Esterases; Female; Histocytochemistry; Liver; Male; Staining and Labeling; Succinate Dehydrogenase; Transaminases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Biopsy; Cell Membrane; Cholestasis; Cytoplasm; Dogs; Humans; Liver; Lysosomes; Microscopy, Electron; Phosphoric Monoester Hydrolases; Rats

Topics: Acid Phosphatase; Animals; Bilirubin; Cholestasis; Glucuronidase; Glutamates; Kidney; Lysosomes; Male; Mitochondria; Nephrosis; Rats; Succinates; Time Factors

Topics: Acid Phosphatase; Acyltransferases; Adenosine Triphosphatases; Alanine Transaminase; Alkaline Phosphatase; Animals; Bilirubin; Biopsy; Cholestasis; Dogs; Esterases; Hemoglobins; Histocytochemistry; Liver; Monoamine Oxidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alanine Transaminase; Aspartate Aminotransferases; Bilirubin; Blood Proteins; Cholestasis; Cholesterol; Creatinine; Diagnosis, Differential; Female; Humans; Iron; Jaundice; Jaundice, Chronic Idiopathic; Postpartum Period; Pregnancy; Pregnancy Complications; Uric Acid; Urinary Tract Infections

Topics: Acid Phosphatase; Acyltransferases; Adenosine Triphosphatases; Bile Acids and Salts; Bilirubin; Binding Sites; Biopsy; Carbon Isotopes; Centrifugation, Density Gradient; Cholestasis; Chromatography, Thin Layer; Dinitrophenols; Fatty Acids; Humans; In Vitro Techniques; Liver; Lysosomes; Microsomes; Mitochondria, Liver; Oligomycins; Oxygen; Taurine

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Bile Ducts, Intrahepatic; Cholestasis; Electron Transport Complex IV; Fatty Liver; Female; Glucose-6-Phosphatase; Histocytochemistry; Hydroxybutyrate Dehydrogenase; L-Lactate Dehydrogenase; Male; Monoamine Oxidase; Oxidoreductases; Rabbits

Topics: Acid Phosphatase; Alkaline Phosphatase; Bile; Cell Nucleus; Cholestasis; Cytomegalovirus Infections; Giant Cells; Hepatitis; Humans; Infant; Infant, Newborn; Jaundice; Liver; Liver Diseases; Liver Regeneration; Pathology