acid-phosphatase and Cholera

As a result of the enteral and combined subcutaneous-enteral immunization of adult rabbits with cholerogen toxoid and the specific fraction of chemical cholera vaccine in tablets immunological transformation occurred in the organism and a "protective" barrier consisting of antibody-containing cells was formed in the intestine on days 3-5. The combined method of immunization with cholera vaccine proved to be essentially as effective as enteral immunization in 2 administrations.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Administration, Oral; Animals; Antibodies, Bacterial; Antibody-Producing Cells; Appendix; Cholera; Cholera Vaccines; Glucosephosphate Dehydrogenase; Injections, Subcutaneous; Lymph Nodes; Peyer's Patches; Plasma Cells; Rabbits; Spleen; Tablets; Vaccination

The cholerogenic effect produced in suckling rabbits by intraintestinal administration of a cholerogen was shown to be accompanied by the edema of villi, formation of lympho-histiocytic infiltrates in the stroma and bases of crypts, hemorrhages in the stroma of the villi and loss of individual enterocytes mostly on tops of the villi. In the large intestine, vacuolation and pyknosis of the epithelial nuclei and edema of the submucosa were observed. The intensity of the cholerogenic effect correlated with the degree of the decline of the activity of succinate dehydrogenase, adenosine triphosphatase, alkaline and acid phosphatases in the intestinal tract. Intraintestinal administration of heat-inactivated cholerogen induced no cholerogenic effect and the enzymatic activity in the intestinal tract declined focally and insignificantly.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adenosine Triphosphate; Alkaline Phosphatase; Animals; Cholera; Enterotoxins; Ileum; Rabbits; Succinate Dehydrogenase; Succinates; Vibrio cholerae

Topics: Acid Phosphatase; Agglutinins; Alkaline Phosphatase; Animals; Antibody Formation; Cholera; Cholera Vaccines; Depression, Chemical; DNA; Doxycycline; Histocytochemistry; Immunity; Liver; Lymph Nodes; Neutralization Tests; Plasma Cells; Rabbits; RNA; Spleen; Succinate Dehydrogenase; Vaccination