acid-phosphatase and Cholelithiasis

Gallstone patients have a reduced cellular lysosome content in the gallbladder mucosa cells compared with gallstone-free subjects. The purpose of the study was to further evaluate the possible role of lysosomes in the pathogenesis of cholesterol gallstone formation in humans.. Lysosomal enzyme activities were assayed in gallbladder mucosa and for comparison in liver specimens of 19 gallstone-free subjects and 24 gallstone patients undergoing cholecystectomy.. Gallstone patients had 25-50% lower activities of the lysosomal proteases cathepsin B, D and L in their gallbladder mucosa compared with gallstone-free subjects. The activity of acid phosphatase also tended to be decreased in gallstone patients. The liver lysosomal enzyme activities were not significantly different between the two groups.. The results show that gallstone patients have diminished lysosomal enzyme activities in the gallbladder mucosa, a finding which may be related to decreased intracellular degradation of proteins and/or mucin in the mucosal cells. This may lead to a higher concentration of mucin in gallbladder bile and thus an increased risk of precipitation of cholesterol crystals and gallstone formation.

Topics: Acid Phosphatase; Bile; Biopsy; Cathepsins; Cholelithiasis; Female; Gallbladder; Humans; Liver; Lysosomes; Male; Middle Aged; Mucins; Mucous Membrane

The histological and histochemical changes of liver from gallstones treated with ACDC are confronted with the same livers before treatment. There is few modification in histological patterns but the frequency of sinusoidal congestion increase. Numerous histoenzymological "injuries" are corrected by this treatment.

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Chenodeoxycholic Acid; Cholelithiasis; Female; Histocytochemistry; Humans; Leucyl Aminopeptidase; Liver; Male; Middle Aged; Oxidoreductases; Transaminases

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Animals; Antibody Formation; Antigens; Cholelithiasis; Female; Glucuronidase; Hexosaminidases; Histocytochemistry; Humans; Lupus Erythematosus, Systemic; Lymph Nodes; Lymphocytes; Male; Peroxidases; Plasma Cells; Rabbits; Rats; Sarcoidosis; Stomach Neoplasms; Stomach Ulcer; Tuberculosis

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Biliary Tract Diseases; Cholecystectomy; Cholelithiasis; Chronic Disease; Colitis, Ulcerative; Diabetes Mellitus; Duodenum; Gastrectomy; Gastritis; Histocytochemistry; Humans; Intestinal Mucosa; Intestine, Small; Jejunum; Leucyl Aminopeptidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Cholelithiasis; Female; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Liver; Male

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Cholecystectomy; Cholelithiasis; Gallbladder; Gallstones; Humans; Microscopy, Electron; Organ Culture Techniques; Oxidoreductases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Cell Biology; Cholecystitis; Cholelithiasis; Electrons; Esterases; Gaucher Disease; Genetics, Medical; Histocytochemistry; Humans; Jaundice; Leukocytes; Microscopy; Microscopy, Electron; Splenectomy; Sulfatases