acid-phosphatase and Cholecystitis

Functional condition of basic components of the microbicidal system (cation protein myeloperoxidase, acid phosphatase) and spontaneous NB-test of neutrophylic leukocytes' activity values were studied with the aid of cytochemical methods in 175 patients: with acute cholecystitis (133) and exacerbation of chronic one (42). As a result of performed examinations various changes of intraleukocytic components and activity of spontaneous HB-test, of neutrophils were established, which depended on phase, severity of the disease and morphological changes and manifestations of pathological process. The determined changes of intracellular components and activity of spontaneous HB-test values of leukocytes in patients with acute and chronic cholecystitis demonstrate the tension of nonspecific reactivity of the organism.

Topics: Acid Phosphatase; Cholecystitis; Follow-Up Studies; Humans; Intracellular Fluid; Leukocytes; Neutrophils; Peroxidase; Prognosis; Severity of Illness Index

Topics: Acid Phosphatase; Adult; Aged; Aged, 80 and over; Blood Bactericidal Activity; Cations; Cholecystitis; Humans; Middle Aged; Neutrophils; Nitroblue Tetrazolium; Peroxidase

Esterases active at neutral and acid pH have been demonstrated in human gallbladder epithelium. Reaction to a wide variety of inhibitors and activators suggests that they probably represent the same enzyme with two active sites while a close correlation with acid phosphatase, a known lysosomal enzyme, suggests a lysosomal localization. Esterase activity in the gallbladder mucosa in significantly decreased in chronic cholecystitis. Polyacrylamide gel electrophoresis suggests that cholesterol stones may be associated with a specific type of isoenzyme pattern. Esterase positive cells are present in the inflammatory infiltrate in the lamina propria of the gallbladder in most cases of chronic cholecystitis. Naphthol AS-D chloracetate esterase staining indicates that mast cells form a large part of the infiltrate. Bile acids modulate the enzyme activity in vitro. Although the exact substrate specificity is not known, several possibilities are discussed, implicating esterases in the pathogenesis of chronic cholecystitis.

Topics: Acid Phosphatase; Carboxylesterase; Carboxylic Ester Hydrolases; Cholecystitis; Electrophoresis, Polyacrylamide Gel; Epithelium; Female; Gallbladder; Humans; Hydrogen-Ion Concentration; Isoenzymes

The lysosomal enzymes beta-glucuronidase and acid phosphatase were studied in 112 patients with cholecystitis. Acid phosphatase activity was generally lower in patients with cholesterol stones compared with cases with pigment stones. beta-glucuronidase activity was higher in acalculous cholecystitis than in any other group, a fact compatible with the concept that in lithiasis the enzyme is secreted into the bile and therefore may participate in nidus formation. Histochemistry at light microscopical level clearly demonstrates the lysosomal distribution of these enzymes and their presence in the macrophages infiltrating lamina propria in cholesterolosis. Electron histochemistry in 45 patients showed acid phosphatase activity in lysosomes and some in mucous droplets. Thiamine pyrophosphatase activity, a marker for the Golgi system, showed a close association with these mucous droplets. The secretion of mucus will be accompanied by a secretion of acid phosphatase, and by implication other acid hydrolases, into the bile.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Cholecystitis; Epithelium; Female; Gallbladder; Glucuronidase; Histocytochemistry; Humans; Lysosomes; Male; Microscopy, Electron; Middle Aged; Thiamine Pyrophosphatase

Under study was the effect of hyperbaric oxygenation in 46 patients on the course of bile tract diseases, complicated with hepatic insufficiency. Hyperbaric oxygenation was found to render a positive effect on the course of hepatic insufficiency.

Topics: Acid Phosphatase; Acute Disease; Aged; Alkaline Phosphatase; Cholecystitis; Cholestasis; Chronic Disease; Electron Transport Complex IV; Female; Glycogen; Humans; Hyperbaric Oxygenation; Liver; Male; Middle Aged; Oxygen Consumption; Succinate Dehydrogenase

Human gallbladder epithelium was homogenized with a view to maintaining the integrity of subcellular components. In such homogenates, N-acetyl-beta-glucosaminidase, beta-glucuronidase, beta-galactosidase, beta-glucosidase, beta-fucosidase, beta-xylosidase, and acid phosphatase were demonstrated together with phospholipase activity. All the enzymes exhibited structure-linked latency. After discarding cellular debris from the homogenate, remaining subcellular organelles were analytically separated by density gradient centrifugation. After 100,000 g for 1 hour, particles containing acid glycosidases were recovered at a sucrose density of 1.18-1.19, whereas the mitochondrial marker enzyme succinate-reductase accumulated at a density of 1.16. The bulk of sedimentable phospholipase activity was recovered with particles sedimenting at 1.18-1.19. The results are interpreted as indicating that phosphalipase is present in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, particularly phospholipase A, from the gallbladder epithelium is discussed as mediation of an inflammatory reaction in the gallbladder, i.e. cholecystitis.

Topics: Acetylglucosaminidase; Acid Phosphatase; Acute Disease; alpha-L-Fucosidase; Cell Fractionation; Cholecystitis; Epithelial Cells; Epithelium; Galactosidases; Gallbladder; Glucosidases; Glucuronidase; Glycoside Hydrolases; Humans; Lysophosphatidylcholines; Lysosomes; Phospholipases

Aseptic inflammation in the gallbladder wall was studied in vitro using rabbit gallbladders. Wall permeability changes, measured as changes in the transmural passage of radioactively labelled hippuran and biligraphin as well as enzyme (acid phosphatase) release from wall to lumen were registered as parameters permitting quantitative assessment of an inflammatory process. Lysolecithin, a surface-active substance of possible significance in the pathogenesis of acute cholecystitis was chosen as inflammation-inducing agent. When 6-20 mg/ml lysolecithin was instilled into the gallbladders, dose-dependent increases in permeability and enzyme release occurred. No effects as compared with controls were seen when 2 mg/ml was instilled. Lysolecithin-treated gallbladders showed morphological signs of inflammation as opposed to untreated. The data reported are consistent with earlier observations that lysolecithin may cause inflammatory lesions to the gallbladder wall. The experimental model used may prove useful in examining the effects of aggressive and protective agents upon the gallbladder mucous membrane.

Topics: Acid Phosphatase; Animals; Capillary Permeability; Cholecystitis; Depression, Chemical; Dose-Response Relationship, Drug; Enzyme Induction; Gallbladder; Iodipamide; Iodohippuric Acid; Lysophosphatidylcholines; Male; Rabbits; Stimulation, Chemical

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Cell Biology; Cholecystitis; Cholelithiasis; Electrons; Esterases; Gaucher Disease; Genetics, Medical; Histocytochemistry; Humans; Jaundice; Leukocytes; Microscopy; Microscopy, Electron; Splenectomy; Sulfatases