acid-phosphatase and Carcinoma-256--Walker

We have studied the effect of 3-amino-1-hydroxypropylidene-1,1-bisphosphonate (APD) on the morphology of rat bone and the metastatic behavior of Walker 256 (W256) cancer cells in the rat skeleton. Male Fischer rats (150-175 g) received s.c. injections for 7 days with APD (0.5 mg/kg body weight/day) (+ APD; n = 20) or with vehicle (-APD; n = 20). Subsequently, 10 + PD and 10 -APD rats received i.m. injections with W256 cells (+ W256), and the remaining rats received injections of vehicle (-W256). All rats were killed 14 days later. Trabecular bone volume was increased by 46 +/- 3% by APD treatment alone and was decreased by 56 +/- 7% (SEM) by W256 tumor burden alone. After 14 days of tumor burden, + APD/+ W256 rats had 3-fold more trabecular bone than did -APD/+W256 rats. Despite this bone-sparing effect, APD treatment of +W256 rats was associated with a 2.6-fold increase in skeletal tumor burden, while metastatic tumor burden in the liver, lungs, and kidneys was unaffected. The increased skeletal tumor burden in + APD/+ W256 rats was accompanied by an increase in the growth rate of W256 cells located in bone. Independent of APD treatment, W256 cells located adjacent to trabecular bone surfaces had greater growth rates than did W256 cells in the marrow, located > 50 microns from trabecular bone. In summary, the APD-induced increase in trabecular bone volume in rats is associated with a selective increase in skeletal tumor burden and an increased growth rate of W256 cells in the skeleton.

Topics: Acid Phosphatase; Animals; Bone and Bones; Bone Neoplasms; Carcinoma 256, Walker; Cell Division; Diphosphonates; Femoral Neoplasms; Male; Pamidronate; Pilot Projects; Rats; Rats, Inbred F344; Thymidine

An increase in the number of hepatocellular lysosomes is detectable with the electron microscope in rats bearing a carcinogen-induced sarcoma but not in rats bearing a transplanted Walker tumour. Biochemical studies show an increase in lysosomal enzymes in the liver of both groups of rats, but the increase in the rats bearing a carcinogen-induced sarcoma was greater than that in rats bearing a Walker tumour. This difference is explained by the fact that only gross increases in lysosomal population are likely to be detected in the small samples examined with the electron microscope, but by biochemical methods whereby many grams of tissue are analysed, finer changes may be detected. The collective data to date indicate that an increase in hepatocellular lysosomes and/or lysosomal enzymes occurs in rats and man bearing a variety of tumours and to this there is, as yet, no known exception.

Topics: Acid Phosphatase; Animals; Carcinoma 256, Walker; Cathepsins; Galactosidases; Glucuronidase; Liver; Lysosomes; Rats; Sarcoma, Experimental

Topics: Acid Phosphatase; Animals; Carcinoma 256, Walker; Cell Nucleus; Cyclophosphamide; Deoxyribonucleases; Glucuronidase; Golgi Apparatus; Inclusion Bodies; Injections, Intraperitoneal; Lysosomes; Male; Microscopy, Electron; Neoplasm Transplantation; Radiation Effects; Rats; Vitamin A

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Carcinoma 256, Walker; Cytoplasm; Injections, Subcutaneous; Male; Microsomes; Mitochondria; NAD; NADP; Neoplasm Transplantation; Rats; Succinate Dehydrogenase; Time Factors; Transplantation, Homologous

Topics: Acid Phosphatase; Animals; Carcinoma 256, Walker; Cathepsins; Female; Galactosidases; Hydrolases; Leukocytes; Neoplasms, Experimental; Rats; Sarcoma, Experimental; Sulfatases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Carcinoma 256, Walker; Cricetinae; Electron Transport Complex II; Esterases; Histological Techniques; Liver Neoplasms; Metabolism; Mice; Neoplasm Transplantation; Oncogenic Viruses; Oxidoreductases; Phosphoric Monoester Hydrolases; Rats; Research; Sarcoma; Sarcoma, Experimental; Skin Neoplasms; Succinate Dehydrogenase