acid-phosphatase and Burns--Chemical

Topics: Acid Phosphatase; Animals; Burns, Chemical; Caustics; Cornea; Eye Burns; Lysosomes; Rabbits; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Cornea; Corneal Injuries; Epithelium; Histocytochemistry; Rabbits; Sodium Hydroxide; Wound Healing

In order to assess the extent of injury and the response of the cornea to alkali burns, NaOH in concentrations of 0.5, 0.25, 0.1, 0.05, and 0.01 N was applied to rabbit eyes and the histologic and metabolic changes studied. The activities of alkaline and acid phosphatases in homogenates and in cold microtome sections were examined on days 1, 4, and 7 after injury. At all time intervals 0.5 N and 0.25 N NaOH induced a remarkable decrease in enzyme activities. On the other hand, after 0.05 N and 0.01 N NaOH only very slight changes were observed. Using 0.1 N NaOH, both phosphatases decreased on day 4 after treatment and acid phosphatase reached normal values in a week, whereas alkaline phosphatase increased with a maximum on day 7. Its role in synthetic processes during corneal regeneration is discussed. Both histologic and metabolic patterns in the experimentally burned cornea were shown to be a function of NaOH concentration and the duration of contact. The process of re-epithelization of the cornea during healing after 0.1 N NaOH for 1 min is described.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Cornea; Corneal Injuries; Eye Burns; Histocytochemistry; Phosphoric Monoester Hydrolases; Rabbits; Regeneration; Sodium Hydroxide

Alkaline and acid phosphatases were studied in the rabbit cornea following acetone and ethanol exposure to the eye. Changes in enzyme activities were investigated in homogenates of epithelium and stroma quantitatively and in frozen cryostat sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings showed a remarkable increase in alkaline phosphatase of the epithelium beginning on day 7. This activation persisted until day 28 after instillation of both noxae with maximum activity on day 14. However, acetone was proved to be significantly more effective than ethanol. In addition, a different topochemistry of alkaline phosphatase was found in the epithelium of treated corneas, i.e., enzyme activity was observed not only superficially but in all epithelial layers of the cornea as compared to a normal one. The effect of acetone and ethanol on a regenerating corneal epithelium is discussed.

Topics: Acetone; Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Cornea; Epithelium; Ethanol; Eye Burns; Histocytochemistry; Rabbits

Topics: Acetylcysteine; Acid Phosphatase; Animals; Burns, Chemical; Corneal Injuries; Cysteine; Eye Burns; Gels; L-Lactate Dehydrogenase; Ophthalmic Solutions; Rabbits

Topics: Acid Phosphatase; Acids; Alkalies; Alkaline Phosphatase; Animals; Burns, Chemical; Eye Burns; Rabbits; Time Factors

Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Burns, Chemical; Eye; Eye Burns; Hydrochloric Acid; Rabbits; Sodium Hydroxide

1. Lymph was collected directly from the hind limbs of rabbits and cats anaesthetized with pentobarbitone.2. Injury to the hind limb was caused by injection of one of two chemicals; either dimethylsulphoxide (DMSO) which produced a mild injury, or croton oil which produced severe injury.3. After subcutaneous injection (6 x 0.2 ml) into the right hind limb there was a good correlation between the changes in concentration of intracellular enzyme systems in the lymph draining the limb and the histological appearance of the injured tissue. DMSO caused an increase in cytoplasmic enzymes only, while after croton oil, which caused cell necrosis, there was an increase in the concentration of all intracellular enzymes examined.4. There was an increased lymph flow accompanied by a fall in protein concentration after DMSO, while croton oil caused an increase in protein concentration and a variable effect on lymph flow.

Topics: Acid Phosphatase; Alanine Transaminase; Animals; Aspartate Aminotransferases; Burns, Chemical; Cathepsins; Cats; Croton Oil; Dimethyl Sulfoxide; Glucuronidase; Hindlimb; L-Lactate Dehydrogenase; Lymph; Potassium; Proteins; Rabbits; Skin