acid-phosphatase and Brucellosis

Investigation of functional-metabolic activity of leukocytes by assessment of basic components of the microbicidal system in the course of chronic brucellosis with reference to the stage, severity, complications and concomitant diseases.. Time course of changes in myeloperoxidase, acid and alkaline phosphatase activity, levels of cation protein, glycogen and lipids in leukocytes were studied in seventy-one 16-70-year-old patients with exacerbation of chronic brucellosis. The diagnosis of primary-chronic, secondary-chronic brucellosis, subcompensation was made in 13, 58 and 69 patients, respectively. 14 patients had chronic infectious inflammatory diseases, 13 patients had chronic non-inflammatory diseases.. Patients with chronic brucellosis at the height of the exacerbation had suppressed activity of myeloperoxidase and lowered level of cationic protein with high activity of acid and alkaline phosphatases, elevated glycogen and lipids in leukocytes. General condition of the patients improved in parallel with multidirectional shifts in the levels of microbicidal system components with normalization at the stage of persistent remission.. Changes in the level of intracellular components of leukocytes depended on brucellosis stage, severity, complications, concomitant diseases, completeness of recovery. This is of clinico-diagnostic significance.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Blood Bactericidal Activity; Brucellosis; Chronic Disease; Female; Glycogen; Humans; Leukocytes; Lipids; Male; Middle Aged; Peroxidase

In experiments on 240 guinea-pigs metabolic changes were found to occur in peripheral blood cells in the process of the development of brucellosis infection and after immunization. The degree and character of the activity of lysosomal enzymes depended on the time of infection and immunity formation. In comparison with the vaccinal culture of Br. abortus 19, the virulent culture of Br. abortus 544 induced greater changes in the activity of esterase, acidic and alkaline phosphatase in neutrophils, in the activity of acidic phosphatase in lymphocytes, as well as in the number of lymphocytes containing this enzyme. The data on enzymatic activity are recommended for use as a differential test for evaluating the character of the infectious and vaccinal processes.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; B-Lymphocytes; Brucellosis; Esterases; Female; Guinea Pigs; Leukocyte Count; Leukocytes; Lymphocytes; Lymphocytes, Null; Male; Neutrophils; T-Lymphocytes; Vaccination

Topics: Acid Phosphatase; Animals; Autoantibodies; Brucellosis; Guinea Pigs; Hemagglutinins; Isoantibodies; Lymph Nodes; Lymphocytes; Skin Tests

Cells of Brucella melitensis strain 16 M were labeled with (32)P. When injected into normal guinea pigs, labeled, viable bacteria were taken up and inactivated in liver and spleen during the 60 min after infection. Both uptake and inactivation increased if brucellae were coated with antibrucella antibody. Neither viability nor radioactivity were lost when labeled brucellae were incubated for 60 min in vitro with normal guinea pig blood, liver homogenates, or in defined medium. Incubation for 12 h with antibrucella rabbit immunoglobulin G similarly was innocuous. Livers were removed from infected animals at various times up to 60 min after injection and were separated into subcellular fractions. The numbers of total (determined by radioactivity measurements) and viable brucellae as well as the acid phosphatase activity in the various fractions were determined. Total bacteria and acid phosphatase activity were progressively transferred from the mitochondrial plus light mitochondrial (M + L) fraction to the nuclear (N) fraction. Viability of brucellae declined more rapidly in the N fraction than in other fractions. Examination of M + L fractions by isopycnic centrifugation showed a decrease in viability of both free brucellae and those in particles. The results indicated the formation of bacteria-containing heterolysosomes which progressively increased in size and in which brucellae were inactivated. The antibrucella activity of phagocytes of guinea pig liver in vivo appeared to be greater than that of peritoneal macrophages from immune rabbits or of bovine leukocytes studied in vitro.

Topics: Acid Phosphatase; Animals; Antibodies, Bacterial; Blood; Brucella; Brucellosis; Cell Fractionation; Centrifugation, Density Gradient; Guinea Pigs; Immunoglobulin G; Liver; Macrophages; Male; Microsomes, Liver; Mitochondria, Liver; Phagocytosis; Phosphorus Radioisotopes; Spleen; Time Factors

The distribution of Brucella melitensis in various tissues and in subcellular fractions obtained from liver was investigated to evaluate the initial phases of brucellosis in the guinea pig. Fifty minutes after intravenous infection, brucellae were found principally in the blood and liver, with a substantial number recovered from spleen. Fractionation of liver established that most bacteria were found in the mitochondrial plus lysosomal (M + L) fraction; a significant number, however, sedimented in the nuclear (N) fraction. With time, there was a progressive shift of bacteria from the M + L to the N fraction, accompanied by a similar shift in acid phosphatase activities. Isopycnic centrifugation of mixtures of M + L fractions and brucellae permitted complete separation of acid phosphatase-bearing particles from bacteria. Similar experiments with fractions from infected animals showed that viable bacteria were found in both the acid phosphatase and free brucellae regions of the gradient. At 10 min postinfection, 52% of the recovered organisms were in the acid phosphatase region; at 30 min, 65%; at 60 min, 85%; and at 315 min, 79%. Detergent plus sonic treatment of an M + L fraction from the liver of an animal killed 50 min after infection caused most of the bacteria in the acid phosphatase region to shift to the region where free bacteria were found. These data suggested that brucellae sequestered in the liver were located primarily in the vacuolar apparatus of the cells which phagocytized them.

Topics: Acid Phosphatase; Animals; Brucella; Brucellosis; Centrifugation, Density Gradient; Culture Media; Cytoplasm; Guinea Pigs; Inclusion Bodies; Injections, Intravenous; Kidney; Liver; Liver Diseases; Lung; Lysosomes; Myocardium; Phagocytosis; Phosphorus Isotopes; Sepsis; Spleen; Subcellular Fractions

Topics: Acid Phosphatase; Animals; Brucellosis; Endotoxins; Histocytochemistry; Hydrocortisone; In Vitro Techniques; Liver; Mice; Mononuclear Phagocyte System; Phagocytosis; Spleen