acid-phosphatase and Bronchitis

The aim of this study was to investigate the effect of heparin on the rat bronchial mucosa changes induced by sulphur dioxide (SO2) inhalation. Sixty five rats were used in this experiment. Five of them constituted a control group, while 60 were exposed to SO2. Forty of the latter subgroup were additionally treated with low molecular weight heparin (LMWH), either during or after terminating exposure to SO2. In all animals exposed to SO2 inflammatory cells were found in broncho-alveolar lavage fluid (BALf) in numbers significantly higher from those observed in healthy controls. The rats exposed to SO2 and treated with LMWH showed intermediate cell pattern in the bronchi between healthy and SO2- exposed animals. When comparing histological picture of the bronchi, we noted extensive changes in irritated rats. These changes were either less expressed or totally absent in animals treated with heparin. The activity of enzymes: acid phosphatase (ACP), alkaline phosphatase (AP) and lactate dehydrogenase (LDH) rose in BALf, although the rise was not parallel and did not correlate with the magnitude of cellular influx or histological changes. Heparin did not influence this changes.

Topics: Acid Phosphatase; Animals; Bronchi; Bronchitis; Bronchoalveolar Lavage Fluid; Disease Models, Animal; Heparin; L-Lactate Dehydrogenase; Male; Mucous Membrane; Partial Thromboplastin Time; Rats; Sulfur Dioxide

In four series of experiments human peripheral blood neutrophils were found capable of synthetizing the active forms of such enzymes as myeloperoxidase and acid phosphatase after stimulation with opsonized zymosan, and the optimum conditions for testing the synthetizing activity of neutrophils were established. The examination of 39 practically healthy donors revealed an approximate equilibrium between the synthesis of the active forms of the enzyme and its excretion from the cell after this cell was activated. Considerable changes in the enzymatic activity of peripheral blood neutrophils were found in patients with yersiniosis, chronic herpes, chronic bronchitis and AIDS-associated complex. The possibility of a deficient enzymatic activity in some patients was suggested.

Topics: Acid Phosphatase; AIDS-Related Complex; Blood Donors; Bronchitis; Cells, Cultured; Dose-Response Relationship, Drug; Herpes Simplex; Humans; Lymphocyte Activation; Neutrophils; Peroxidase; Reference Values; Time Factors; Yersinia Infections

The dynamics of acid phosphatase and granulocytic elastase activities in bronchoalveolar washings of 40 patients with chronic catarrhal bronchitis was studied. In case of effective cure, the activities of these enzymes were increased shortly on the 10-13 days from beginning of the cure. In order to study the nature of this enzymes, the additional researches were conducted. These researches demonstrated that the macrophages were the source of the acid phosphatase, and the neutrophils--elastase. The transitory increase of the activity of both enzymes is favourable prognostical sign of disease's course.

Topics: Acid Phosphatase; Bronchitis; Bronchoalveolar Lavage Fluid; Clinical Enzyme Tests; Granulocytes; Humans; Macrophages; Neutrophils; Pancreatic Elastase; Prognosis; Research; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Bronchitis; Humans; Lymphocyte Activation; Lymphocytes

A study was made of the level, viability and functional activity of alveolar macrophages (AM) in lavage of 86 patients with chronic bronchitis (26 with nonobstructive and 60 with obstructive bronchitis). A decrease in AM viability by 11-25%, phagocytic activity 1.6-4.6-fold, the NBT-test 1.5-3.4-fold and an increase in acid phosphatase activity more than 2-fold were revealed. The degree of a decrease in AM activity depended on the presence of bronchial tree obstruction and the purulent nature of inflammation. Treatment (a total of 33 patients were examined) resulted in an increase in AM content and viability however AM functional properties did not return to normal completely, the rate of positive shifts in activity indices did not correlate with clinical manifestations. An analysis of the dependence of AM functional activity on the type of drug therapy showed that tetracycline inhibited AM functional activity, ampicillin made a favorable effect on the restoration of AM function and beclomet stimulated significantly AM activity.

Topics: Acid Phosphatase; Adolescent; Adult; Bronchitis; Cell Survival; Chronic Disease; Combined Modality Therapy; Female; Humans; Macrophages; Male; Middle Aged; Oxidation-Reduction; Phagocytosis; Pulmonary Alveoli; Therapeutic Irrigation

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Bronchitis; Cathepsin D; Endopeptidases; Humans; Lysosomes; Macrophages; Middle Aged; Neutrophils; Pulmonary Alveoli; Pulmonary Fibrosis

The aim of this investigation was to increase the accuracy of the determination of the functional activity of alveolar macrophages with phagocytic functions, isolated from bronchial washings, to reduce the time necessary for this determination and to compare the results thus obtained with the clinical signs of the disease. 46 patients with nonspecific pulmonary diseases were examined. The method of the simultaneous evaluation of the phagocytic activity of alveolar macrophages and the level of acidic phosphatase activity during phagocytosis was found to ensure greater accuracy in the determination of the functional activity of alveolar macrophages and to reduce the time necessary for the evaluation of the reaction by 1.5 hours. In patients with non-specific pulmonary diseases the phagocytic activity of alveolar macrophages decreased, which was accompanied by a lower level of acidic phosphatase consumption.

Topics: Acid Phosphatase; Adolescent; Adult; Bronchitis; Humans; Lung Diseases; Macrophages; Middle Aged; Phagocytosis; Pneumonia; Pulmonary Alveoli

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Bronchitis; Chronic Disease; Enzyme Activation; Humans; Leukocytes; Lymphocyte Activation; Middle Aged; Neutrophils; Peroxidases

Topics: Acid Phosphatase; Adult; Bronchial Neoplasms; Bronchitis; Chronic Disease; Female; Glucuronidase; Humans; Male; Ribonucleases; Sputum

The results of cytological examinations of sputum in 100 patients with chronic bronchitis in the period of exacerbration of the disease and in 70 patients with limited chronic inflammatory process in the lung clinically interpreted either as chronic pneumonia or pneumosclerosis are compared. Considerable accumulations of round-cell elements of inflammatory infiltrate with predominance of monocytes as well as predominant exfoliation of transitory bronchial epithelium and not ciliated epithelium were most frequently found in the group of patients with limited chronic inflammatory process in the lung. Fragments of atypical bronchial epithelium simulating cancer elements were found mostly in patients with chronic bronchitis showing marked an asthmatic component. In the course of exacerbation of chronic asthmatic bronchitis accompanied by positive clinical dynamics, there was an increase in the size of alveolar macrophages in the sputum and increase in the content of hydrolytic enzymes, acid phosphatase and nonspecific esterase, in them.

Topics: Acid Phosphatase; Adult; Bronchitis; Chronic Disease; Esterases; Female; Histocytochemistry; Humans; Male; Middle Aged; Pneumonia; Pulmonary Fibrosis; Sputum

Topics: Acid Phosphatase; Bronchiectasis; Bronchitis; Chronic Disease; Fluorescent Antibody Technique; gamma-Globulins; Glucosephosphate Dehydrogenase; Glycosaminoglycans; Histiocytes; Humans; Lung; Lung Abscess; Macrophages; Microscopy, Fluorescence; Plasma Cells; Pneumonia; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adolescent; Bronchitis; Child; Child, Preschool; Chronic Disease; Humans; Leukocytes; Pneumonia; Recurrence

The measurement of acid and alkaline phosphatase and cathepsin activities in bronchial aspirates obtained through bronchoscopy from a series of 75 patients suggests a procedure that may have value as a routine diagnostic examination. Using this approach, seven patients with neoplasms in the lung showed elevated levels of alkaline phosphatase and cathepsin in bronchial aspirates without elevation in acid phosphatase activity.

Topics: Acid Phosphatase; Alkaline Phosphatase; Bronchi; Bronchial Neoplasms; Bronchitis; Bronchoscopy; Cathepsins; Humans; Isoenzymes; Lung Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Bronchitis; Chronic Disease; Clinical Enzyme Tests; Humans; Neutrophils; Occupational Diseases; Pneumoconiosis

Topics: Acid Phosphatase; Adolescent; Asthma; Bronchiectasis; Bronchitis; Child; Child, Preschool; Chronic Disease; Cystic Fibrosis; Glucuronidase; Glycosaminoglycans; Hexosaminidases; Humans; Macrophages

Topics: Acid Phosphatase; Alkaline Phosphatase; Bronchi; Bronchitis; Histocytochemistry; Humans; Mucous Membrane