acid-phosphatase and Biliary-Tract-Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; alpha-Amylases; Biliary Tract Diseases; Clinical Enzyme Tests; Enzymes; Female; gamma-Glutamyltransferase; Humans; Isoenzymes; L-Lactate Dehydrogenase; Lipase; Liver Diseases; Metabolism, Inborn Errors; Myocardial Infarction; Nucleotidases; Pancreas; Transaminases; Uterine Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Aspartate Aminotransferases; Biliary Tract Diseases; Cardiovascular Diseases; Clinical Enzyme Tests; Creatine Kinase; Esterases; Glucosephosphate Dehydrogenase; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Muscular Diseases; Neoplasms; Skin Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; Aspartate Aminotransferases; Biliary Tract Diseases; Cardiovascular Diseases; Clinical Enzyme Tests; Creatine Kinase; Esterases; Glucosephosphate Dehydrogenase; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Muscular Diseases; Neoplasms; Skin Diseases

Some factors that influence the values of respiratory activities of liver mitochondria isolated from surgical biopsy specimens have been studied. By sedimentating of mitochondria at a lower centrifugal force (5,500 g) than usually used for rat liver mitochondria, and washing the mitochondrial pellet twice, the contamination with lysosomes and microsomes was lowered. At 37 degrees C, and in the presence of hexokinase and glucose, the oxygen uptake was greater than at 25 degrees C and in their absence. The respiratory control was good and the respiratory activities were rather stable during the first 3-4 h after isolation. The respiratory activities of mitochondria isolated from patients with duodenal or gastric ulcers, biliary diseases, and subjects with no digestive diseases (all having normal liver) were compared. Differences in oxygen uptake and acceptor control index values with some substrates were noted. The conditions for selection of controls in studies on subcellular fractions of human liver include: absence of any hepatic antecedents; no clinical evidence of liver involvement; no abnormality in routine liver function tests; a histologic aspect free of pathological conditions, and a normal aspect of the tissue during the homogenization and the fractionation procedure (absence of steatosis or fibrosis). These data provide a basis for the standardization of methods in establishing the reference values of mitochondrial activities for the modifications in a variety of diseases.

Topics: Acid Phosphatase; Biliary Tract Diseases; Glucose; Glucose-6-Phosphatase; Hexokinase; Humans; Isocitrates; Liver; Lysosomes; Malates; Microsomes, Liver; Mitochondria, Liver; Oxygen Consumption; Pyruvates; Succinates; Temperature; Ulcer

Topics: Acid Phosphatase; Biliary Tract Diseases; Clinical Enzyme Tests; Histidine Ammonia-Lyase; Humans; L-Lactate Dehydrogenase; Liver Diseases; Urokinase-Type Plasminogen Activator

Topics: Acid Phosphatase; Adult; Aged; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Biliary Tract Diseases; Estradiol Dehydrogenases; Female; Humans; Liver; Male; Middle Aged; Neuroleptanalgesia; Pancreatic Neoplasms; Peptic Ulcer; Transketolase

Topics: Acid Phosphatase; Alkaline Phosphatase; Amylases; Biliary Tract Diseases; Bone Diseases; Clinical Enzyme Tests; Creatine Kinase; Enzymes; Humans; Isoenzymes; Liver Diseases; Male; Muscular Diseases; Myocardial Infarction; Nervous System Diseases; Organ Specificity; Oxidoreductases; Pancreatic Diseases; Prostatic Diseases; Pulmonary Embolism; Transaminases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Biliary Tract Diseases; Cholecystectomy; Cholelithiasis; Chronic Disease; Colitis, Ulcerative; Diabetes Mellitus; Duodenum; Gastrectomy; Gastritis; Histocytochemistry; Humans; Intestinal Mucosa; Intestine, Small; Jejunum; Leucyl Aminopeptidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Bile Ducts; Biliary Tract Diseases; Bone and Bones; Carcinoma, Hepatocellular; Chemical and Drug Induced Liver Injury; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis; Intestines; Isoenzymes; Kidney; Ligation; Liver; Liver Diseases; Liver Neoplasms; Male; Neoplasms, Experimental; Rats

Topics: Acid Phosphatase; Biliary Tract Diseases; Blood Platelets; Bone and Bones; Breast Neoplasms; Erythrocytes; False Positive Reactions; Female; Formaldehyde; Humans; Hydrogen-Ion Concentration; Kidney; Kidney Diseases; Liver; Liver Diseases; Male; Neoplasms; Phenolphthaleins; Phosphates; Prostate; Prostatic Diseases; Prostatic Neoplasms; Tartrates

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Bile Ducts, Intrahepatic; Biliary Tract Diseases; Bilirubin; Common Bile Duct; Histocytochemistry; Liver; Male; Nucleotidases; Rats; Thiocyanates

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Bile; Bile Ducts, Intrahepatic; Biliary Tract Diseases; Histocytochemistry; Jaundice; Liver; Liver Glycogen; Male; Nucleotidases; Rats; Thiocyanates

Topics: 5'-Nucleotidase; Acid Phosphatase; Alkaline Phosphatase; Biliary Tract; Biliary Tract Diseases; Bone Diseases; Disease; DNA; Leucyl Aminopeptidase; Liver Diseases; Pancreas; Pancreatic Diseases; Peptide Hydrolases; Phosphoric Monoester Hydrolases; Protein Tyrosine Phosphatases; RNA; Trees