acid-phosphatase and Asthma

Although corticosteroid resistant (CR) bronchial asthma is associated with impaired in vitro an din vivo glucocorticoid (GC) responsiveness in mononuclear cell function, it is not known whether this is a cell specific phenomenon or whether these patients are at risk of glucocorticoid side-effects as corticosteroid-sensitive (CS) subjects. In order to address this question we have examined the effects of GCs on biochemical indices of bone turnover in vivo.. Six CS and six CR subjects received prednisolone 40 mg orally at 09.00 daily for 5 days. At 08.30 on day 1 and day 6 a fasting blood was taken for estimation of serum osteocalcin, tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP; total and bone isoenzyme), and a urine sample taken for estimation of free deoxy-pyridinoline crosslinks. TRAP and ALP were measured by a colorimetric method and osteocalcin and deoxy-pyridinoline crosslinks by ELISA.. Serum osteocalcin (nmol/L) decreased from 1.39 +/- 0.09 (mean +/- SEM) to 1.14 +/- 0.07 (P = 0.023) and from 1.19 +/- 0.05 to 0.96 +/- 0.1 (P = 0.037) in the CS and CR groups respectively. There was no difference between baseline and post-treatment levels in either group. Serum total ALP (units/L) decreased from 178 +/- 7 to 168 +/- 4 (P = 0.02) and from 195 +/- 22 to 175 +/- 16 (P = 0.04) in the CS and CR groups, respectively. There was no difference between baseline and post-treatment levels in either group. There was no significant elevation of deoxy-pyridinoline crosslinks or TRAP and no suppression of bone ALP in either group.. We suggest that CR asthmatics may be equally at risk from the metabolic side-effects of glucocorticoids.

Topics: Acid Phosphatase; Adrenal Cortex Hormones; Alkaline Phosphatase; Asthma; Bone Regeneration; Bone Resorption; Drug Resistance; Humans; Osteocalcin; Prednisolone

Neutrophils (PMN) from 20 patients with atopic disease (atopy), the parents of 8 of the patients and 10 normal controls were studied by light-microscopic cytochemistry. The results revealed that myeloperoxidase (MPO) and acid phosphatase (ACP) activities in PMN in all cases significantly decreased and alkaline phosphatase activity was normal. Parents or parent of 7 of those patients had PMN enzyme deficiency similar to that of the patients. The results indicated that a primary combined and partial deficiency of MPO and ACP in PMN azurophilic granule existed in atopy. It is postulated that the deficiency led to reduction of PMN bactericidal power and delay of bactericidal action. Foreign bodies which were partially degraded could possess antigenic property. This is believed to be the important cytobiological mechanism of the tendency toward infections and formation of sensitive antigen in atopy.

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Asthma; Child; Child, Preschool; Dermatitis, Atopic; Female; Humans; Male; Middle Aged; Neutrophils; Peroxidase; Rhinitis, Allergic, Perennial

Previous studies have shown that inhaled corticosteroids can affect bone metabolism in adults. A study to assess the effect of inhaled beclomethasone, 300 to 800 micrograms/day for at least 6 months (mean 25 months), was therefore undertaken in children. In part 1 of the study, 18 children with asthma, aged 4 to 17 years (mean 10.1 years), were compared with an age- and sex-matched group of children with asthma not treated with corticosteroids. In part 2, eight more pairs were compared. Comparisons were also made with 61 healthy children. Bone mineral density measured by radiographic absorptiometry, and bone mineral content measured by single-photon absorptiometry and by dual-energy x-ray absorptiometry, showed no significant differences. Serum levels of calcium, magnesium, zinc, total alkaline phosphatase, bone specific alkaline phosphatase, parathyroid hormone, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D also showed no differences. The activity of tartrate-resistant acid phosphatase, a marker of bone resorption, was significantly lower in the beclomethasone group than in both the asthma control and the normal control groups, but urine calcium excretion did not differ. Patients with asthma had lower serum osteocalcin and higher serum copper levels than control subjects without asthma, but treatment with beclomethasone did not affect these values. We conclude that inhaled beclomethasone (up to 800 micrograms/day) does not reduce bone mineralization or increase bone resorption. Effects on bone formation were difficult to assess because asthma per se caused a significant reduction in osteocalcin, a sensitive marker of bone formation.

Topics: Acid Phosphatase; Administration, Inhalation; Adolescent; Asthma; Beclomethasone; Bone and Bones; Bone Density; Case-Control Studies; Child; Child, Preschool; Copper; Female; Humans; Male; Minerals; Osteocalcin; Phosphorus; Time Factors

Two groups of patients with bronchial asthma (of average severity and a severe course) and a group of healthy persons were examined. Changes in dehydrogenase activity in the time course of asthma development associated with the depletion of the adaptation mechanisms, and the compensation of these disorders as a result of glucocorticosteroid therapy were established. AP activity in neutrophils could serve as an informative sign indicative of asthma progression and showing the efficacy of hormonotherapy. Various shifts in the metabolic activity of enzymes ensuring the efficacy of phagocytosis with relation to a degree of asthmatic symptoms and therapeutic modalities were revealed.

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminophylline; Asthma; Creatine Kinase; Humans; Hydrocortisone; Lymphocytes; Neutrophils; Oxidoreductases; Peroxidase; Protein Kinases

To evaluate the role of proteolytic balance in atopic bronchial asthma, the activity of lysosomal enzymes and the concentration of their natural inhibitors were determined in bronchoalveolar lavage fluid and serum in 28 patients with asthma. The results were statistically analyzed. A significant increase of lysosomal enzyme activities as compared with healthy volunteers was detected mainly in bronchoalveolar lavage fluid. Similarly, a selective increase in the concentration of the natural inhibitors of proteolytic enzymes, especially alpha-2-macroglobulin, was noted. Moreover, the concentrations of alpha-1-antitrypsin and the activities of neutral protease, alpha-1-antitrypsin, alpha-2-macroglobulin and acid protease were correlated. These findings suggest the participation of lysosomal enzymes and their natural inhibitors in immune response during atopic bronchial asthma. The protease antiprotease balance control may be used in prognostic and therapeutic procedure in diseases of the respiratory tract.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Asthma; Blood Proteins; Enzyme Inhibitors; Female; Humans; Hydrolases; Lung; Lysosomes; Male; Peptide Hydrolases; Reference Values

A parallel study of the activity of serum cholinesterase and excretion of cyclic guanosine monophosphate made it possible to distinguish 3 types of disorders of cholinergic regulation in exacerbation of bronchial asthma: cholinergic compensation, subcompensation and decompensation. The nature of correlations of the degree of disturbance of cholinergic regulation, intensity of allergic reaction and its type was established. The role and place of the choline blocking agents in multiple modality therapy of bronchial asthma were defined.

Topics: Acid Phosphatase; Acute Disease; Adult; Aged; Asthma; Cholinesterases; Cyclic GMP; Female; Histamine; Humans; Immunoglobulin E; Male; Middle Aged; Status Asthmaticus

A total of 114 patients with infections-allergic bronchial asthma were studied, being subdivided into two groups. The first group covers 53 patients without clinical, immunologic, X-ray, enzymatic and biochemical sings of active inflammation. The second group includes 61 patients with manifested bronchial-pulmonary inflammation. Immunoglobulins of classes IgA, IgM, and IgG, complement enzymatic activity of total acid phosphatase were studied as well as sorbitoldehydrogenase and "acute phase" proteins. The values of immunoglobulin classes IgA and IgG, coplement, enzymatic activity of total acid phosphatase and sorbitodechydrogenase and "acute phase" proteins were established to be with regularly increased values with high statistical significance. Those indices could serve the differentiation and making the diagnosis of acute and exacerbated pulmonary inflammation as well as for prescribing the adequate antiinflammatory treatment of the patients with infectious-allergic bronchial asthma.

Topics: Acid Phosphatase; Acute Disease; Adolescent; Adult; Aged; Alpha-Globulins; Asthma; Clinical Enzyme Tests; Complement System Proteins; Female; Humans; Immunoglobulins; L-Iditol 2-Dehydrogenase; Male; Middle Aged; Sialic Acids

Topics: Acid Phosphatase; Allergens; Asthma; Enzyme Activation; Glucuronidase; Humans; Lysosomes; Muramidase; Neutrophils; Rhinitis, Allergic, Seasonal; Ribonucleases

Topics: Acid Phosphatase; Adult; Asthma; Female; Glucuronidase; Humans; Infections; Male; Middle Aged; Neutrophils; Phagocytosis

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Asthma; Atmospheric Pressure; Female; Glycerolphosphate Dehydrogenase; Humans; Leukocytes; Male; Middle Aged; Peroxidase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Allergens; Asthma; Desensitization, Immunologic; Humans; Leukocytes; Pollen

Topics: Acid Phosphatase; Adolescent; Adult; Antigens, Bacterial; Asthma; Bacterial Vaccines; Child; Child, Preschool; Humans; In Vitro Techniques; Infant; Infant, Newborn; Leukocytes; Middle Aged

It was found that the levels of CH50, C3, complement component. lysozyme, beta-D-glucuronidase, and acid phosphatase were increased in blood serum of patients with asthma bronchiale during a climatic treatment in the highmountain condition of Strbské Pleso (altitude 1,350 m). The increase of CH50 was monophasic, while the changes of other factors were biphasic--increasing at the beginning and falling down to the starting values at the end of the treatment. The changes observed are a part of the adaptation of organism to the high-mountain condition.

Topics: Acid Phosphatase; Adult; Asthma; Climate; Complement C3; Female; Glucuronidase; Humans; Lysosomes; Male

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Asthma; Female; Glucuronidase; Humans; Leukocytes; Lysosomes; Male; Middle Aged; Muramidase; Ribonucleases

Topics: Acid Phosphatase; Acute Disease; Adult; alpha-Macroglobulins; Asthma; Humans; Lymphocyte Activation; Lymphocytes; Male; Remission, Spontaneous

Topics: Acid Phosphatase; Adolescent; Adult; Asthma; Cromolyn Sodium; Glucuronidase; Humans; Leukocytes; Middle Aged

Topics: Acid Phosphatase; Adolescent; Adult; Antigen-Antibody Complex; Asthma; Female; Humans; Immune Complex Diseases; Immunoglobulins; Lysosomes; Male; Middle Aged

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Asthma; Female; Glycerolphosphate Dehydrogenase; Glycerophosphates; Humans; Leukocytes; Male; Middle Aged; Peroxidase; Succinate Dehydrogenase; Succinates

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Asthma; Female; Glycerolphosphate Dehydrogenase; Humans; Leukocytes; Male; Middle Aged; Seasons; Succinate Dehydrogenase

Topics: Acid Phosphatase; Asthma; Histocytochemistry; Humans; Lung Diseases; Lung Neoplasms; Neutrophils; Sarcoidosis

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Asthma; Child; Child, Preschool; Glycerolphosphate Dehydrogenase; Humans; Infant; Succinate Dehydrogenase

The phenotypic distribution and gene frequencies of haptoglobin (Hp), transferrin (Tf), group specific component (Gc), cholinesterase (Cho E2), and alpha1-antitrypsin (Pi) in plasma proteins, and phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase ((6-PGD), esterase D (Es D), phosphohexose isomerase (PHI), adenosine deaminase (ADA) and acid phosphatase (AcP) in red cells were studied in 127 atopic, asthmatic patients. The gene frequencies were compared with normal groups. The phenotypic distribution of the Pi system in atopic patients was somewhat different from the normal. No significant differences were found between the two groups in protein systems or in enzyme systems, except Pi systems. In conclusion, except for the Pi system, no definite association between polymorphic traits and atopic asthma was found in this study.

Topics: Acid Phosphatase; alpha 1-Antitrypsin; Asthma; Cholinesterases; Erythrocytes; Esterases; Gene Frequency; Glucose-6-Phosphate Isomerase; Haptoglobins; Humans; Phenotype; Phosphoglucomutase; Phosphogluconate Dehydrogenase; Polymorphism, Genetic; Transferrin

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Asthma; Child; Child, Preschool; Glycerolphosphate Dehydrogenase; Humans; Infant; Leukocytes; Succinate Dehydrogenase

Topics: Acid Phosphatase; Acute Disease; Adult; Allergens; Asthma; Bacteria; Chronic Disease; Clinical Enzyme Tests; Humans; Hypersensitivity; Leukocytes; Middle Aged; Remission, Spontaneous; Skin Tests

Topics: Acid Phosphatase; Adolescent; Asthma; Bronchiectasis; Bronchitis; Child; Child, Preschool; Chronic Disease; Cystic Fibrosis; Glucuronidase; Glycosaminoglycans; Hexosaminidases; Humans; Macrophages

Topics: Acetylcholinesterase; Acid Phosphatase; Air Conditioning; Asthma; Catalase; Cholinesterases; Climate; Erythrocytes; Female; Humans; Male; Mining; Neutrophils; Sodium Chloride

Topics: Acid Phosphatase; Adenosine Triphosphatases; Asthma; Child; Humans; Transaminases

Topics: Acid Phosphatase; Alkaline Phosphatase; Asthma; Dermatitis, Atopic; Eosinophils; Exudates and Transudates; Histocytochemistry; Humans; Injections, Intravenous; Leukocytes; Pyrogens; Skin

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Asthma; Child; Child, Preschool; Cholinesterases; Colorimetry; Enzymes; Humans; Lipase

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Asthma; Clinical Enzyme Tests; D-Alanine Transaminase; Emphysema; Fructose-Bisphosphate Aldolase; Humans; Ornithine; Oxidoreductases; Pulmonary Emphysema; Sweden