acid-phosphatase and Ascites

Topics: Acid Phosphatase; Ascites; Chyle; Drainage; Humans; Hypertension, Portal; Kidney Failure, Chronic; Lymph; Pancreatitis; Shock, Hemorrhagic; Sympathetic Nervous System; Thoracic Duct

Ascites sarcoma 180 (S180A) is a transplantable tumor maintained in ddY mice. In the tumor-bearing mice, the plasma Ca, Pi and acid phosphatase levels increased and the plasma alkaline phosphatase levels decreased. The elevation of plasma Pi levels is unusual in humoral hypercalcemia of malignancy (HHM). To characterize the pathogenesis of HHM in the animals, the biological activities in the serum-free conditioned media (CM) of S180A cell cultures were examined. The S180A CM stimulated bone resorption dose dependently and showed TGF-like, IL-1-like and mitogenic activity. Unlike parathyroid hormone (PTH), the factor(s) failed to stimulate cAMP production by either UMR 106-01 cells or neonatal mouse calvaria at concentrations that stimulate bone resorption. Also, the factor(s) stimulated proliferation of UMR 106-01 cells concomitant with a slight increase in intracellular calcium levels. These results indicate that S180A cells produce a factor(s) responsible for bone resorption which is apparently different from PTH-like activity.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Ascites; Bone Resorption; Calcium; Culture Media; Cyclic AMP; Hypercalcemia; Interleukin-1; Mice; Mitogens; Neoplasm Transplantation; Osteoclasts; Parathyroid Hormone; Phosphorus; Sarcoma 180; Transforming Growth Factors; Tumor Cells, Cultured

A carcinoma invasion system (Krebs-2 and Ehrlich tetraploid ascites tumors invading mouse peritoneum) was studied by high-voltage electron microscope (HVEM) stereoscopy, conventional (medium voltage) electron microscopy (MVEM), and cytochemistry. Tumor cells entered areas of peritoneum (mainly parietal) only where mesothelial cells were damaged and where there was inflammation of the underlying stroma. The initial invasion was different from that of most other invading carcinomas in that there was minimal breakdown of basal lamina and collagen. Neither tumor cells, inflammatory leukocytes nor peritoneal fibroblasts showed significant secondary lysosome production or release of intracellular or extracellular acid phosphatase. Morphological and cytochemical criteria suggest that in some invading carcinomas, as with non-tumor migrating cells such as leukocytes, widespread proteolysis due to diffusion of proteases is not a prerequisite for invasion of stromal connective tissue.

Topics: Acid Phosphatase; Animals; Ascites; Basement Membrane; Carcinoma; Collagen; Extracellular Matrix; Inflammation; Lysosomes; Mice; Peptide Hydrolases; Peritoneal Neoplasms

Electrophoresis and ion-exchange column chromatography were used to separate the wide varieties of acid phosphatases with different biological and clinical significance. Band 0 was very strong in ascitic cells with many autophagic vacuoles, indicating a role in autophagic function. Band 1 was a membrane-bound acid phosphatase, seen mainly in the microsomal fraction. Band 3 was the major lysosomal acid phosphatase of all nonprostatic tissues. Bands 2 and 4 were antigenically identical to each other, and were observed in unusually high amounts in the prostate. The different electrophoretic mobility between bands 2 and 4 was due to their carbohydrate content. Band 5 was a characteristic enzyme of the osteoclast. The tartrate-sensitive enzymes included bands 0 through 4. Only band 5 was tartrate resistant. The tartrate-resistant acid phosphatase of erythrocytes was not detected by the electrophoresis method. Clinical applications were seen for both bands 2 and 5. Band 2 was a secretory enzyme, normally secreted into the seminal plasma. Band 2 was absorbed into the blood circulation in some prostatic cancer patients. A small amount of bands 2 and 4 was observed in nonprostatic tissues. The diagnostic value of band 2 resulted from its extremely high concentration in the prostate. Band 5 was not observed in the normal prostate. A high concentration of band 5 was observed in hairy cells, Gaucher cells, and osteoclasts. The serum level of band 5b was an indicator of osteoclastic activity in the bone. Elevation of band 5b in serum was observed in normal children during physiological bone growth, in Gaucher's disease, and in malignancies metastasized to bone.

Topics: Acid Phosphatase; Animals; Ascites; Bone Neoplasms; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Female; Gaucher Disease; Humans; Leukemia L1210; Male; Mice; Osteoclasts; Phagocytes; Prostate; Prostatic Neoplasms; Tartrates

Appreciable numbers of glycogenosomes were found in ascites hepatoma cells. They were lined by a single or sometimes double membranous structure exhibiting weakly positive acid phosphatase activity. Some were closely related to the Golgi apparatus, and a few showed wrapping of glycogen particles. The glycogen extracted from ascites hepatoma AH 13 cells was of two major types: a normal adult liver type, and a muscle type. Only the latter type gave the Cotton effect on measurement of the ORD and CD spectra. Small particulate, muscle type glycogen was observed in the glycogenosomes. Autophagy of normal adult liver type glycogen was never found. The engulfment of muscle type glycogen was accompanied by high acid and neutral alpha-glucosidase activity levels.

Topics: Acid Phosphatase; alpha-Glucosidases; Animals; Ascites; Glycogen; Golgi Apparatus; Liver Neoplasms, Experimental; Male; Microscopy, Electron; Rats

In our attempts at establishing a cancer cell line from various ascites of cancer bearing patients, a cell line was successfully established from the ascites of a 63-year-old female with primary ovarian tumor (embryonal carcinoma). Histological findings of the peritoneum, due to metastasis, appeared to be cystadenocarcinoma, revealing the differentiation to non-epithelial cells which formed coarse networks and fibers, and morphologic changes of tissue cultures also reflected such histologic findings. At present the subculture has reached the 95th population doubling level, and cultured cells have assumed the morphology of mesothelial cells or fibroblasts with about 50 chromosomes. As a human malignant cell line, it is useful for the study of human malignant tumor cell.

Topics: Acid Phosphatase; Alkaline Phosphatase; Ascites; Autopsy; Basophils; Cell Line; Cell Transformation, Neoplastic; Chromosomes; Culture Techniques; Cytoplasm; Female; Humans; Middle Aged; Neoplasm Metastasis; Ovarian Neoplasms; Peritoneal Neoplasms; Phagocytosis; Teratoma

Topics: Acid Phosphatase; Adenocarcinoma; Ascites; Biopsy; Humans; Male; Middle Aged; Neoplasm Metastasis; Peritoneal Neoplasms; Prostatic Neoplasms; Radiography; Urinary Catheterization

Topics: Acid Phosphatase; Animals; Ascites; Cathepsins; Cell Membrane; Endoplasmic Reticulum; Epithelium; Esterases; Histiocytes; Histocytochemistry; Liver; Liver Neoplasms; Lymphocytes; Lysosomes; Mice; Microscopy, Electron; Mitochondria, Liver; Organoids; Plasma Cells; Ribosomes; Sarcoma, Experimental

Topics: Acid Phosphatase; Animals; Ascites; Carcinoma, Hepatocellular; Cell Line; Cytoplasm; Glycogen; Histocytochemistry; Liver; Liver Neoplasms; Lysosomes; Microscopy, Electron; Neoplasms, Experimental; Rats

Topics: Acid Phosphatase; Animals; Ascites; Glucosephosphate Dehydrogenase; Histocytochemistry; Lysosomes; Mice; Mitochondria; Neoplasm Transplantation; Neoplasms, Experimental; Organoids; Succinate Dehydrogenase; Transplantation, Homologous

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Ascites; Creatine; Humans; Jaundice; Liver Cirrhosis; Male

Topics: Acid Phosphatase; Adenosine Triphosphate; Alkaline Phosphatase; Alkylating Agents; Animals; Antineoplastic Agents; Ascites; Benzoquinones; Biochemical Phenomena; Biochemistry; Carbohydrate Metabolism; Carcinoma, Ehrlich Tumor; Cholinesterases; Hexokinase; Metabolism; Mice; NAD; Pharmacology; Quinones; Research

Topics: Acid Phosphatase; Animals; Ascites; Deoxyribonucleases; DNA; Histocytochemistry; Lysosomes; Mitomycin; Mitomycins; Neoplasms; Pathology; Pharmacology; Rats; Research; Sarcoma, Yoshida