acid-phosphatase and Appendicitis

The activities of leucine amino peptidase (LAP) and acid phosphatase (Acid-P), conceivable markers of acute appendicitis, were determined in the portal blood of rabbits with acute appendicitis. An experimental model of acute appendicitis was established using No.-O silk ties to block the base of the appendix. The clinical and histopathological picture of acute appendicitis was seen after 12 hr in all the rabbits in the model group (9/9) and in none of the control group. Catheterization of the superior mesenteric vein was performed in rabbits with acute appendicitis, and portal blood samples were taken at 0, 6, and 12 hr for assay of LAP and Acid-P activities. No statistically significant difference between the experimental group and the control group, in the activities of LAP and Acid-P, was found at any time interval. The experimental model of acute appendicitis in the rabbit which is described here is simple and carries a high rate of success. This is probably the first report of using continuous catheterization and repeated sampling of portal blood, for the measurement of enzyme activities, in an experimental model of acute appendicitis. It was concluded that serum LAP and Acid-P activities cannot be used as markers for acute appendicitis.

Topics: Acid Phosphatase; Acute Disease; Animals; Appendicitis; Disease Models, Animal; Female; Leucyl Aminopeptidase; Male; Portal Vein; Rabbits

Topics: Acid Phosphatase; Adolescent; Appendicitis; Chemotaxis, Leukocyte; Child; Child, Preschool; Humans; Infant; Neutrophils; Peroxidase; Phagocytosis

The appendectomy specimens submitted to surgical pathology exhibit a wide variety of morphological appearances. We have evaluated the activities of acid phosphatase, alpha-naphthyl esterase, and leucineaminopeptidase in 100 human appendixes divided into six age groups in order to ascertain whether any histochemical pattern was related to the age group rather than to pathological condition. Our findings support that the submucosal fibrosis is unrelated to the patient's age, as well as appendiceal obliteration. It is noteworthy that the mucosa of the appendix may retain its lymphoid constituents at any age and that young subjects may already show appearances of involution up to lumen obliteration.

Topics: Acid Phosphatase; Adolescent; Adult; Age Factors; Aged; Appendicitis; Appendix; Carboxylic Ester Hydrolases; Child; Female; Histocytochemistry; Humans; Leucyl Aminopeptidase; Male; Middle Aged; Naphthol AS D Esterase

We have histochemically evaluated the LAP, AP and ANE activities of the human appendix in 100 surgical specimens. The mucosa has been particularly investigated for its different aspects during inflammatory conditions. Two main facts have been ascertained: the subepithelial location of macrophages, and the AP and ANE activities of two groups of cells in the germinal centers. Subepithelial macrophages show conspicuous AP activity that correlates with the degree of inflammatory response and of exposure to luminal antigens. In the germinal centers, AP and ANE activities disclose two further groups of positive cells, probably the same cells reacting to both technics. One group is rooted to the core of the germinal center; the other to the intermediate area between the germinal center and the follicular mantle.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Appendectomy; Appendicitis; Appendix; Child; Enzyme Activation; Epithelium; Female; Humans; Leucyl Aminopeptidase; Lymphoid Tissue; Macrophages; Male; Middle Aged; Naphthol AS D Esterase

To discriminate initial stages of an inflammation from its destructive forms, the authors studied the leucocyte lysosome activity at different stages of inflammatory processes in abdominal organs. 90 patients and 30 practically healthy individuals were examined. The indices of acid and base phosphotase activity were found to rise together with the progress of an inflammatory process. A nomogram, permitting to determine with a rather high precision the stage and direction of the process, was made up on the grounds of the established reguliarity after the mathematical procession of the obtained figure data.

Topics: Abdomen, Acute; Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Appendicitis; Cellulitis; Enzyme Activation; Female; Gangrene; Humans; Leukocyte Count; Leukocytes; Lysosomes; Male; Middle Aged

Topics: Acid Phosphatase; Adrenocorticotropic Hormone; Aging; Alkaline Phosphatase; Animals; Anura; Appendicitis; Autonomic Nervous System; Cell Differentiation; Cortisone; Digestive System; Dogs; Electron Transport Complex IV; Esterases; Estrone; Fishes; Guinea Pigs; Histocytochemistry; Humans; Methods; Methyltestosterone; Myenteric Plexus; Neurons; Nucleic Acids; Polysaccharides; Protein Biosynthesis; Pyloric Stenosis; Radiation Injuries, Experimental; Rats; Sex Factors; Stomach Ulcer; Succinate Dehydrogenase