acid-phosphatase and Anemia

Topics: Acid Phosphatase; Alkaline Phosphatase; Amino Acids; Aminopeptidases; Anemia; Blood Proteins; Carbohydrate Metabolism; Catalase; Esterases; Fructose-Bisphosphate Aldolase; Glucose-6-Phosphatase; Glucuronidase; L-Lactate Dehydrogenase; Lactates; Lipid Metabolism; Malate Dehydrogenase; Neoplasms; Phosphoglucomutase; Phospholipids; Proteins; Transaminases

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Anemia; Bone Marrow Cells; Child; Cholesterol; Clinical Trials as Topic; Corneal Opacity; Female; Humans; Lipid Metabolism, Inborn Errors; Lipoproteins; Male; Methods; Middle Aged; Pedigree; Phospholipids; Proteinuria; Triglycerides; Uric Acid

We have prospectively studied the evolution of serum levels of the prostatic-specific antigen and prostatic acid phosphatase in 14 male hemodialyzed patients, receiving a cycle of nandrolone decanoate (200 mg intramuscularly, once a week, for six months) as treatment for anemia. Androgen administration did not produce significant increases in serum concentrations of both tumor markers (basal: 0.9 +/- 0.5 and 0.7 +/- 0.3 ng/ml; at six months: 1.3 +/- 1.1 and 0.8 +/- 0.7 ng/ml respectively). Only one patient had a value of prostatic-specific antigen over the normal range: 4.2 ng/mol at the sixth month period, with a rapid decrease after the withdrawal of androgens. All the remaining values of both markers were within the normal range. In another six patients undergoing a prolonged treatment with androgens (between 9 to 24 months), the serum levels of prostatic-specific antigen and prostatic acid phosphatase were within the normal range in all of them. Nandrolone decanoate administration does not induce increases in prostate tumor markers when it is used as treatment for anemia in hemodialyzed patients.

Topics: Acid Phosphatase; Aged; Aged, 80 and over; Anabolic Agents; Anemia; Dose-Response Relationship, Drug; Drug Administration Schedule; Hematocrit; Hemoglobinometry; Humans; Injections, Intramuscular; Kidney Failure, Chronic; Male; Middle Aged; Nandrolone; Nandrolone Decanoate; Prospective Studies; Prostate; Prostate-Specific Antigen; Renal Dialysis

Topics: Acid Phosphatase; Anemia; Child, Preschool; Electroencephalography; Female; Gaucher Disease; Glucosylceramidase; Hexosaminidases; Humans; Injections, Intravenous

Infectious salmon anaemia (ISA) is a disease of farmed Atlantic salmon (Salmo salar L.) in Norway that affects both erythrocytic and leucocytic cells. Both cell types are possible target cells for the aetiological ISA agent, which is probably a virus. In the present study the distribution and phenotype of leucocyte populations in the spleen and head kidney of Atlantic salmon that were developing ISA have been examined. Frozen tissues were collected from fish at various times after inoculation with ISA-infective material. Immune and enzyme histochemical techniques were used to characterise the response of leucocyte populations. Acid phosphatase positive macrophages predominantly in the red pulp of the spleen appeared to have engulfed erythrocytes at day 4 after infection. Evidence of degradation products of phagocytosed erythrocytes was present in macrophages in red pulp of the spleen at day 7 after infection, in addition to the usual site of erythrophagocytosis in melanomacrophage accumulations. Signs of erythrophagocytosis were not found in the head or body portions of the kidney. The activation of macrophages in the spleen at day 7 was suggested by decreased reactivity for the enzyme 5' nucleotidase. From day 7, clusters of immunoglobulin positive (Ig +) cells were present in the head kidney, while from day 11, the ellipsoids of the spleen showed reactivity for Ig and complement factor C3. These observations are discussed in relation to early immunoglobulin production and possible immune complex trapping. The present results suggest that the leucocyte populations in Atlantic salmon respond to ISA infection through macrophage activation and the initiation of an immune response.

Topics: Acid Phosphatase; Anemia; Animals; Carboxylesterase; Carboxylic Ester Hydrolases; Erythrocytes; Fish Diseases; Hematocrit; Histocytochemistry; Immunoglobulins; Immunohistochemistry; Kidney; Leukocytes; Macrophage Activation; Phagocytosis; Salmon; Spleen; Time Factors

Gaucher disease is the most frequent lysosomal storage disease and the most prevalent genetic disease among the Ashkenazi Jews (q approximately 0.047). The disease results from inherited defects of acid beta-glucosidase and the accumulation of the substrate, glucosylceramide, in cells of monocyte/macrophage origin. The therapeutic response to macrophage-targeted (alpha-mannosyl-terminated) alglucerase (Ceredase, at 60 to 15 IU/kg every 2 weeks) was analyzed in 33 patients (age range, 2 to 63 years; 15 splenectomized) with extensive Gaucher disease over periods of 6 to 24 months. The efficacy of several different doses and dosage reductions was evaluated. In patients with anemia (n = 30) and/or thrombocytopenia (n = 19), hemoglobin levels and platelet counts increased by 0% to 178% and 15% to 155%, respectively, within 3 to 12 months. In patients with splenomegaly (n = 17) and/or hepatomegaly (n = 28), liver and spleen volumes decreased in 6 months from 7% to 64% and 8% to 84% by 12 months, respectively. Hematologic and visceral improvements were noted at any doses between 60 and 15 IU/kg every 2 weeks. Furthermore, these positive initial therapeutic responses were persistent throughout therapy, with doses reduced by 50%. Pulmonary Gaucher disease did not improve clinically in 3 patients. Unrelated cirrhotic (n = 2), cholestatic (n = 1), or renal disease (n = 1) did not influence the rate of patient improvement. Two of five patients who developed serum antibodies against alglucerase during the first 6 to 12 months of therapy had mild antibody reactions. This study shows similar regression of clinical Gaucher disease manifestations with enzyme therapy, using doses between 30 and 60 IU/kg every 2 weeks. Therapeutic efficacy was not diminished after 50% to 75% dose reductions or in the presence of anti-enzyme antibodies.

Topics: Acid Phosphatase; Adolescent; Adult; Anemia; Bone Diseases; Child; Child, Preschool; Gaucher Disease; Glucosylceramidase; Hepatomegaly; Humans; Kidney Diseases; Liver Diseases; Lung Diseases; Middle Aged; Peptidyl-Dipeptidase A; Splenectomy; Splenomegaly; Thrombocytopenia

1. A cytoplasmic nucleotidase, which preferably hydrolyzed 5'-dUMP, was investigated in rat spleen. 2. Total activity of the nucleotidase increased about 3-fold in the spleen of anemic rat was caused by phenylhydrazine administration. This increase was repressed by the injection of methotrexate, an inhibitor of DNA synthesis. 3. Activities of heme oxygenase or acid phosphatase did not correlate to the change of the nucleotidase activity. 4. The nucleotidase catalyzed dephosphorylation of 3'(5')-dUMP, 3'(5')-dTMP and 3'-UMP more readily, in the presence of Mg2+. Its optimum pH was around 6.0-6.5. It was stimulated by the addition of deoxyinosine. 5. These catalytic properties and tissue distribution of the enzyme, abundant in the thymus, spleen and intestine, were similar to that of 5'(3')-nucleotidase in rat liver [Fritzson P. (1978) Adv. Enzym. Regul. 16, 43-61]. 6. A possible physiological significance of the nucleotidase is in reutilization of preformed pyrimidine nucleotides.

Topics: Acid Phosphatase; Anemia; Animals; Cytoplasm; Heme Oxygenase (Decyclizing); Hydrogen-Ion Concentration; Male; Nucleotidases; Phenylhydrazines; Pyrophosphatases; Rats; Rats, Inbred Strains; Spleen; Thymidine Kinase; Uridine Monophosphate

Topics: Acid Phosphatase; Alcoholism; Anemia; Bone Marrow Cells; Erythrocyte Indices; Erythropoiesis; Female; Fetal Alcohol Spectrum Disorders; Folic Acid Deficiency; Hemolysis; Humans; Megaloblasts; Mitochondrial Swelling; Naphthol AS D Esterase; Pregnancy; Pyridoxal Phosphate

The activity of hexokinase, glucose-6-phosphoric dehydrogenase, lactic dehydrogenase, succinic dehydrogenase, acid and alkaline phosphatases was determined in the rat kidney tissue with phenylhydrazine anemia and posttransfusion polycytemia. The blood supply of the cortical and medullary layers of the kidneys was studied at the same time. The purpose of this work was to ascertain possible connections between the changes in the activity of the enzymes under study with the renal erythropoietin producing function of the kidneys. The blood supply of the kidneys of rats with phenylhydrazine anemia was sharply decreased, but it was markedly elevated in case of posttransfusion polycytemia. There were no significant changes in the activity of the mentioned enzymes. These data suggest that the activity of the kidney enzymes is not a controlling factor in the renal erythropoietin production.

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia; Animals; Blood Transfusion; Glucosephosphate Dehydrogenase; Hexokinase; Kidney; Kidney Cortex; Kidney Medulla; L-Lactate Dehydrogenase; Male; Phenylhydrazines; Polycythemia; Rats; Regional Blood Flow; Succinate Dehydrogenase

On account of 2 own observations, main clinical and diagnostic features of Hairy cell leukemia (HCL) will be discussed. HCL is a rare, unusual type of chronic leukemia and is predominantly particular of middle-aged men. The occurrence of middle-sized lymphoid cells having a hairy cytoplasmic edge, and a tartrate-resistant acid PHOsphatase isoenzyme are the characteristic criteria of the HCL. The diffuse infiltration by hairy cells affecting primarily the spleen and bone marrow results in anaemia, granulocytopenia, thrombocytopenia and splenomegaly. Differential diagnosis have to be made in relation to other lymphatic leukemias, leukemic malignant lymphomas and monoclonal gammopathies as well as lymphotropic viral infections. Immunologic behaviour of hairy cells is like that of B-lymphocytes. Therefore, the term "leukemic reticuloendotheliosis" should no longer be applied.

Topics: Acid Phosphatase; Adult; Age Factors; Agranulocytosis; Anemia; B-Lymphocytes; Bone Marrow; Diagnosis, Differential; Humans; Leukemia; Leukemia, Myeloid; Lymphatic Diseases; Male; Middle Aged; Sex Factors; Spleen; Splenomegaly; Thrombocytopenia

Topics: Acid Phosphatase; Anemia; Histocytochemistry; Humans

Topics: Acid Phosphatase; Anemia; Animals; Binding Sites; Biological Transport, Active; Blood Proteins; Calcium; Cattle; Cyanides; Female; Hydrazones; Iron; Iron Radioisotopes; Kinetics; Lanthanum; Male; Mitochondria; Organ Specificity; Oxygen Consumption; Protein Binding; Rabbits; Receptors, Drug; Reticulocytes; Serum Albumin, Bovine; Succinate Dehydrogenase; Time Factors

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Anemia; Animals; Bacterial Infections; Blood Cell Count; Cerebrovascular Disorders; Female; Gastroenteritis; Histocytochemistry; Humans; Male; Middle Aged; Myocardial Infarction; Neoplasms; Neutrophils; Rabbits; Virus Diseases

Topics: Acid Phosphatase; Anemia; Animals; Erythrocytes; Insect Vectors; Insecta; Iron-Dextran Complex; Lizards; Periodic Acid; Pinocytosis

Topics: ABO Blood-Group System; Acid Phosphatase; Anemia; Body Weight; Electrophoresis; Erythroblastosis, Fetal; Erythrocytes; Female; Germany, East; Humans; Hyperbilirubinemia; Infant; Infant, Newborn; Infant, Premature; Nitrophenols; Phosphates; Pneumonia; Pregnancy; Starch; Umbilical Cord

Topics: Acid Phosphatase; Adult; Anemia; Cholesterol; Corneal Opacity; Electrophoresis; Esters; Female; Humans; Lysophosphatidylcholines; Metabolism, Inborn Errors; Phospholipids; Plasma; Proteinuria; Transferases; Triglycerides

Topics: Acid Phosphatase; Aldehyde-Lyases; Alkaline Phosphatase; Anemia; Animals; Aspartate Aminotransferases; Carbon Tetrachloride; Erythrocyte Count; Erythrocytes, Abnormal; Erythropoiesis; Glucosephosphate Dehydrogenase; Hepatitis; Humans; L-Lactate Dehydrogenase; Oxidoreductases; Phenylhydrazines; Rabbits; Reticulocytes

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia; Animals; Bone and Bones; Cartilage, Articular; Chickens; Male; Microscopy; Nutrition Disorders; Osteoblasts; Vitamin A

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia; Antineoplastic Agents; Child; Child, Preschool; Hematologic Diseases; Humans; Infant; Leukemia; Purpura

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia; Bone Marrow Examination; Collagen Diseases; Esterases; Histiocytosis, Langerhans-Cell; Histocytochemistry; Iron; Microscopy, Electron; Mononuclear Phagocyte System

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Blood Cell Count; Blood Platelets; Bone Marrow Examination; Clinical Enzyme Tests; Diagnosis, Differential; Gaucher Disease; Geriatrics; Humans; Liver Cirrhosis; Pathology; Phosphates; Splenomegaly

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Azo Compounds; Blood Cells; Bone Marrow Cells; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Mycosis Fungoides; Neoplasms; Polycythemia Vera; Sarcoma

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Blood; Humans; Purpura; Purpura, Thrombocytopenic; Thrombocytopenia

Topics: Acid Phosphatase; Anemia; Biochemical Phenomena; Erythrocytes; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Anemia; Anemia, Megaloblastic; Phosphoric Monoester Hydrolases