acid-phosphatase and Anemia--Aplastic

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Antibody-Dependent Cell Cytotoxicity; DNA Nucleotidylexotransferase; Female; Hematologic Diseases; Histocytochemistry; Humans; Infectious Mononucleosis; Leukemia, Hairy Cell; Leukemia, Lymphoid; Lymphoma; Male; Middle Aged; Phenotype; Skin Neoplasms; T-Lymphocytes

Latency of Epstein-Barr virus infection may be generated by surviving immortalized B cells or by continuous re-infection. EBV-positive B-cell tumors have been found following bone-marrow transplantation (BMT) and were of donor type in the few cases investigated. We established a B-cell line from the bone marrow of a patient in complete remission following allogeneic BMT for aplastic anemia 18 months post-grafting. Differences in sex and isoenzymes allowed an exact determination of chimerism in our case. While the patient showed persistent complete chimerism of all cell lineages, cells grown in culture were of recipient type. They expressed B-cell markers, showed a monoclonal rearrangement of the immunoglobulin genes and carried EBV-associated antigens. As direct preparations of cells from the patient did not contain detectable recipient-type cells, it appeared likely that small numbers of EBV-transformed B cells of the recipient survived for long periods in this patient. For the development of secondary B-cell neoplasms in vivo, additional patho-physiological steps like severe graft versus host disease or T-cell suppression are obviously required because the patient was still free of lymphoma 3 years post-grafting.

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Antigens, Viral; B-Lymphocytes; Bone Marrow Transplantation; Capsid; Chimera; Epstein-Barr Virus Nuclear Antigens; Female; Herpesvirus 4, Human; Humans; Karyotyping; Phenotype; Tumor Virus Infections

We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-beta-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all controls (mean 9.4% of erythroblasts). The percentage of positive erythroblasts in pathological conditions varied greatly from absence to over 70%. Acid phosphatase and N-acetyl-beta-glucosaminidase were positive in pernicious anaemia and in acute erythremic myelosis. Nonspecific esterase was only detected in a case of erythremic myelosis. This case also showed a faint metachromasia with the dye azure A.

Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaline Phosphatase; Anemia, Aplastic; Erythroblasts; Esterases; Humans; Leukemia, Erythroblastic, Acute

Quantitative cytochemistry of components of blood neutrophil azurophilic granules (myeloperoxidase, chloroacetate esterase, beta-glucuronidase, and acid phosphatase) and specific granules (lactoferrin) has been performed by scanning and integrating microdensitometry in 13 patients with a myelodysplastic syndrome and 11 patients with chronic granulocytic leukaemia. Both patient groups showed a reduction of enzyme activity in azurophilic granules, and also of lactoferrin, consistent with abnormal development of neutrophil granules. These cytochemical changes in blood neutrophils are similar to those found in acute myeloid leukaemia, are consistent with a leukaemic maturation defect, and may be of diagnostic value.

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Carboxylic Ester Hydrolases; Cytoplasmic Granules; Densitometry; Glucuronidase; Histocytochemistry; Humans; Hydrolases; Lactoferrin; Lactoglobulins; Leukemia, Myeloid; Leukocyte Count; Neutrophils; Peroxidase; Peroxidases

Topics: Acid Phosphatase; Adolescent; Adult; Anemia, Aplastic; Female; Granulocytes; Histocytochemistry; Humans; Male; Middle Aged; Peroxidases

We have previously reported on the ultrastructure of platelets in preleukemia and myelomonocytic leukemia. We referred to an unusual and distinct anomaly of the platelet granules found in 15 of 16 patients. In the present communication we wish to describe and illustrate the light microscopic appearance of giant anomalous granules. Close scrutiny of the platelet morphology and a search for the aforementioned platelet granulopathy are important in the evaluation of patients with myeloproliferative diseases. In this paper we describe and illustrate in more detail the ultrastructure and ultrastructural histochemistry of the abnormal granules. In those patients with the platelet granulopathy, we have conducted in vitro platelet aggregation studies and carried out an electron microscopic evaluation of the aggregates. At least some of the giant granules remained morphologically intact in advanced stages of the aggregation phenomenon, and thus they are probably composed of elements that were not released during aggregation.

Topics: Acid Phosphatase; Anemia, Aplastic; Blood Platelets; Cell Membrane; Cytoplasmic Granules; Humans; Leukemia, Myeloid; Peroxidases; Platelet Aggregation; Precancerous Conditions; Vacuoles

Topics: Acid Phosphatase; Adult; Anemia, Aplastic; Blood Cell Count; Bone Marrow; Cyclophosphamide; Cytoplasm; Female; Hepatomegaly; Histocytochemistry; Humans; Leukemia; Liver; Liver Function Tests; Lymph Nodes; Lymphatic Diseases; Male; Microscopy, Electron; Middle Aged; Prednisone; Reticulin; Ribosomes; Spleen; Splenectomy; Splenomegaly

Topics: Acid Phosphatase; Alkaline Phosphatase; Anemia, Aplastic; Bone Marrow; Disease; Hemochromatosis; Histocytochemistry; Humans; Hypersplenism; Leukocyte Count; Lymphatic Diseases; Methods; Mononuclear Phagocyte System; Multiple Myeloma; Neutrophils; Staining and Labeling

Topics: Acid Phosphatase; Anemia, Aplastic; Blood Platelets; Humans; Leukemia, Myeloid; Megakaryocytes; Purpura, Thrombocytopenic; Spherocytosis, Hereditary; Thalassemia

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Blood Cell Count; Blood Platelets; Bone Marrow Examination; Clinical Enzyme Tests; Diagnosis, Differential; Gaucher Disease; Geriatrics; Humans; Liver Cirrhosis; Pathology; Phosphates; Splenomegaly

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Azo Compounds; Blood Cells; Bone Marrow Cells; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Mycosis Fungoides; Neoplasms; Polycythemia Vera; Sarcoma

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Blood; Humans; Purpura; Purpura, Thrombocytopenic; Thrombocytopenia